Eggshell membrane ameliorates hepatic fibrogenesis in human C3A cells and rats through changes in PPARγ-Endothelin 1 signaling

Abstract

Our previous nutrigenomic findings indicate that eggshell membrane (ESM) may prevent liver fibrosis. Here we investigated the effects and mechanisms underlying ESM intervention against liver injury by using DNA microarray analysis and comparative proteomics. In vitro hydrolyzed ESM attenuated the TGFβ1-induced procollagen production of human hepatocyte C3A cells and inhibited the expression of Endothelin 1 (EDN1) and its two receptors, and extracellular matrix components. In vivo male Wistar rats were allocated into a normal control group, a CCl4 group (hypodermic injection of 50% CCl4 2×/wk) and an ESM group (20 g ESM/kg diet with CCl4 injection) for 7 wks. Dietary ESM ameliorated the elevated activity of ALT/AST, oxidative stress and collagen accumulation in liver, accompanied by the down-regulated expression of Edn1 signaling and notable profibrogenic genes and growth factors as well as peroxisome proliferator-activated receptor gamma (PPARγ). Concomitantly, the decreased expressions of Galectin-1 and Desmin protein in the ESM group indicated the deactivation of hepatic stellate cells (HSCs). Through a multifaceted integrated omics approach, we have demonstrated that ESM can exert an antifibrotic effect by suppressing oxidative stress and promoting collagen degradation by inhibiting HSCs' transformation, potentially via a novel modulation of the PPARγ-Endothelin 1 interaction signaling pathway.

The Possible Protective Role of Powder Cuttlefish Bone, Crabshell and Eggshell on Osteoporotic Rats

“…(Jiaa et al, 2013). These outcomes were in accordance with Jia et al, (2014) who found that dietary ESM displayed potential impacts on hepatic damage and fibrosis induced by CCl4 in rats.…”

“…Kim and Yoon, (2008) proposed that supplementation with chitosan oligosaccharide organization was compelling in the avoidance and change of the lipid level, lowering plasma cholesterol and chemical exercises in serum of hypercholesterolemic rats. Jiaa et al,(2013) and Jia et al, (2014) who indicating that ESM is a sheltered and common side-effect of egg preparing which has intense defensive biochemical capacities against liver damage and fibrosis. ESM may have a cholesterol cutting down effect that can cover liver harm, and at last liver fibrosis.…”

Eggshell membrane powder ameliorates intestinal inflammation by facilitating the restitution of epithelial injury and alleviating microbial dysbiosis

“…The hepatic proteome analysis was conducted as described 8 in Supplemental Methods . A cut-off for p -values < 1.0 and confidence thresholds >1.25 were included for the identification of unique proteins using the IPA classification system.…”

“…We previously obtained nutrigenomic information from in vivo and in vitro investigations of ESM, and we observed biochemical functions of ESM that counter liver injury and fibrosis. These protective effects of ESM appear to be based on the regulation of proteins and genes that are related to the activation of hepatic stellate cells and fibrogenesis, via a potential novel modulation of the PPARγ-endothelin 1 interaction signaling pathway 8 .…”

Systematic Chemical Analysis Approach Reveals Superior Antioxidant Capacity via the Synergistic Effect of Flavonoid Compounds in Red Vegetative Tissues

“…At the end of the 5-week experimental period, after 12 h of starvation, all rats were anesthetized with intraperitoneal chloral hydrate (300–350 mg/kg) at the same time of the day to avoid circadian metabolic fluctuations. Blood samples were obtained from the carotid artery and centrifuged immediately at 800 × g for 15 min at −4°C to separate the serum samples (Jia et al, 2014 ). All study procedures performed on the animals were approved by the Animal Care and Use Ethics Committees of China Agricultural University, and all rats received humane care in accordance with the guidelines.…”