Egg yolk enriched with polyunsaturated fatty acids (PUFAs) improves the shelf life of ram semen in liquid storage

Zarei, Morteza; Rostami, Behnam; Masoumi, Reza; Sharafi, Mohsen; Shahir, Mohammad Hossein; Stear, M. J.; Catt, Sally

doi:10.1016/j.smallrumres.2018.05.002

Cited by 16 publications

(11 citation statements)

References 50 publications

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“…Both ingredients significantly affect sperm motility and membrane integrity. Egg yolk protects spermatozoa against cold shock, and it is widely used to preserve the semen of various animal species [ 44 , 45 , 46 , 47 ]. Cold shock causes considerable damage to plasma membranes, mitochondria, and acrosomes [ 45 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Different Extenders on the Quality Characteristics of European Red Deer Epididymal Sperm Stored at 5 °C

Dziekońska

Neuman

Burdal

et al. 2022

Animals

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The aim of this study was to evaluate the effect of different extenders on the quality of European red deer epididymal sperm stored at 5 °C. Epididymal spermatozoa were collected post mortem from 10 stags and diluted with three extenders (Bovidyl®, BoviFree®, and BioXcell®) and stored at 5 °C. Sperm motility (TMOT), motility parameters (system CASA), plasma membrane integrity (SYBR-14+/PI−), acrosomal membrane integrity (FITC-PNA−/PI−), mitochondrial activity (JC-1/PI), viability, and apoptotic-like changes (YOPRO/PI) were evaluated. The analyses were conducted on the first and successive days of storage (D1–D7). The applied extender, storage time, and the interactions between these factors significantly (p < 0.001) affected most of the analyzed parameters whose values were highest in sperm samples stored in Bovidyl®, regardless of storage time. In Bovidyl®, BoviFree®, and BioXcell® extenders, TMOT values were estimated at 83%, 63%, and 59%, respectively, on D3. The extenders significantly influenced DNA integrity on D7. The percentage of dead sperm increased from D3. The quality of stored sperm cells was significantly influenced by the extenders’ biochemical composition. BoviFree® and BioXcell® contain glycerol which could contribute to deteriorating the quality of spermatozoa stored at 5 °C. Sperm cells stored in the egg yolk-based extender (Bovidyl®) were characterized by the highest viability and functionality.

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Section: Discussionmentioning

confidence: 99%

The Effect of Different Extenders on the Quality Characteristics of European Red Deer Epididymal Sperm Stored at 5 °C

Dziekońska

Neuman

Burdal

et al. 2022

Animals

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“…The recent studies stated that antioxidants, such as taurine, added to semen extenders appeared to increase the frozen-thawed sperm features. Also, consider as sulfonic amino acid, which irritated the sperm plasma membrane, constrain lipid peroxidation and therefore protect spermatozoa (Balao da Silva et al, 2010;Zarei et al, 2018). Results in the 2 nd experiment in the present study declared that, the addition of taurine as an antioxidant at levels of 25 or 50 mM to extender before freezing caused different effects during freezing stages.…”

Section: Discussionmentioning

confidence: 53%

Influence of Dried Egg Yolk with or without Taurine in Extender on Sperm Characteristic of Cryopreserved Buffalo Semen

Sakr

Abdallah

El-Hamady

et al. 2021

Journal of Animal and Poultry Production

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The objective of this study is to determine the influence of dried egg yolk (DEY) powder on freezability of semen buffalo as an alternative of fresh egg yolk (FEY) with or without taurine. Semen was collected from five buffalo bulls with total motility (≥70% mass motility), pooled, extended (1: 10) with different tris extenders containing 20% FEY versus 5, 10, 15 and 20% DEY (1st experiment). Tris-EY extender contained 15% DEY was supplemented with taurine (0, 25, 50, 75 and 100 mM) in 2nd experiment. Results showed that increases in progressive motility at concentration 10 , 15% than 20% in DEY and FEY respectively, after dilution, equilibration, and thawing, Also, acrosome integrity percentage in post-equilibrated and thawed semen was improved (P <0.05) by 5, 10, and 15% DEY as compared with FEY, being the highest with 15% DEY. Adding 25 or 50-Mm taurine improved progressive motility after dilution, and acrosome integrity after equilibration, and membrane integrity percentage after all stages of cryopreservation. the powdered egg yolk at concentration of 10 or 15% may be used, as an alternative of fresh egg yolk in the diluents of buffalo sperm freezing. Addition of taurine at a level of 25 and 50 mM has possible protective effects as an antioxidant to improve semen quality of buffalo semen extended with 15% dried egg yolk.

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“…Adding n-3 linolenic FA can raise PUFA levels in the sperm membrane ( 69 ), improving membrane fluidity and sperm motility. Adding PUFAs to EY can extend shelf life of preserved ram semen ( 70 ). PUFAs increase the fluidity and cryo-tolerance of sperm membrane ( 71 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of adding mixed chicken and quail egg yolks to the cryodiluent on the quality of ram semen before and after cryopreservation

et al. 2022

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The effects of adding mixed chicken and Japanese quail egg yolks (EYs) to the cryodiluent on the quality of ram semen before freezing and post-thawing were evaluated. Additionally, the composition of chicken and quail egg EYs and their mixture were analyzed for results explanation. The semen was collected from rams (n = 5) and extended with cryodiluent containing the EY of chicken, quail or their mixture (1:1). The extended semen was chilled slowly to 5 °C within 2 h and equilibrated for 2 h, before frozen on the liquid nitrogen vapor and cryopreserved at −196 °C. The straws were evaluated before freezing and post-thawing for sperm motility, vitality and abnormality besides plasma-membrane and DNA integrities. The moisture, ash, protein, and fatty acid (FA) contents of chicken EY, quail EY and their mixture were analyzed. Sperm vitality, plasma membrane integrity and DNA integrity before freezing were significantly (P < 0.05) higher in quail EY than chicken EY and mixed EYs cryodiluent. The chicken EY extender significantly improved the vitality, plasma membrane and DNA integrities of post-thawed ram semen in comparison with quail EY or mixed EYs extenders. While, the post-thawing sperm abnormalities was lower (P ≤ 0.05) in quail EY than chicken EY and mixed EYs cryodiluent. The post-thawing sperm motion kinetics parameters were higher in quail EY than chicken EY and mixed EYs cryodiluent. The highest percentages of moisture, ash, saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) were detected in quail EY had. While, the highest percentages of fat, protein and polyunsaturated fatty acids (PUFAs) were detected in chicken EY. In conclusion, using of chicken EY can improve total motility, vitality, plasma membrane integrity and DNA integrity of cryopreserved ram semen. While, using of quail EY can improve sperm abnormalities and kinetic motion parameters of cryopreserved ram semen. Mixing chicken and quail EYs added no value for post-thawing ram semen parameters.

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Egg yolk enriched with polyunsaturated fatty acids (PUFAs) improves the shelf life of ram semen in liquid storage

Cited by 16 publications

References 50 publications

The Effect of Different Extenders on the Quality Characteristics of European Red Deer Epididymal Sperm Stored at 5 °C

The Effect of Different Extenders on the Quality Characteristics of European Red Deer Epididymal Sperm Stored at 5 °C

Influence of Dried Egg Yolk with or without Taurine in Extender on Sperm Characteristic of Cryopreserved Buffalo Semen

Effects of adding mixed chicken and quail egg yolks to the cryodiluent on the quality of ram semen before and after cryopreservation

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