Egg Shell Quality III: Calcium and phosphorus requirements of commercial Leghorns

Roland, D. A.

doi:10.1079/wps19860012

Cited by 46 publications

(44 citation statements)

References 54 publications

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“…The average weight of eggs from kampung chicken, Cihateup duck, and commercial laying hens were 38.11±3.52, 58.93±3.27, and 59.86±0.15 g, respectively. The weight of eggs from commercial laying hens was considerably normal according to Roland et al (1984) and Tugiyanti & Iriyanti (2012). Despite weight of eggs of kampung chicken was significantly lighter (P<0.01) to that of eggs of commercial laying hens, the value was close to the range of average weight of egg sof kampung chicken, which is ranging from 39 to 48 g (Sulandari et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks

Wulandari

Fardiaz²,

Budiman³

et al. 2015

Med Pet

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Egg white lysozyme (EWL) has considerably a wide functional protein exhibiting antibacterial activity mainly against Gram-positive bacteria. The EWL is widely applied in food industry and is considerably safe. Despite its high potency, EWL of Indonesian poultry has never been studied and exploited. This study was aimed to purify EWL from two Indonesian poultry: kampung chicken and Cihateup duck, and compared to egg of commercial laying hens. The eggs in this study were obtained from field laboratory of Faculty of Animal Science, Bogor Agricultural University (IPB) and classified in AA quality based on the interior quality. First attempt to purify the EWL was performed by using ethanol precipitation yielding purified EWL which was still contaminated by other proteins, hence designated as partially purified EWL. Final concentrations of partially purified EWL of kampung chicken, commercial laying hens, and Cihateup duck were about 5800, 5400, and 5500 μg/mL, respectively. To confirm whether the use of ethanol in the purification affecting EWL antibacterial activities, the activities were examined against Staphylococcus aureus. It demonstrated that the partially purified EWL exhibited ability to inhibit S. aureus at 6 and 26 h suggesting that the method was feasible as it did not interfere EWL antibacterial activities. Yet, based on SDS-Page, purity was the issue in ethanol precipitation method. Further attempt using ion exchange chromatography at pH 10 successfully purified lysozyme as indicated by a single band corresponding to lysozyme size (~14 kD) free from bands of other proteins. Altogether, a single step of ion exchange chromatography is sufficient and promising to isolate EWL from Indonesian poultry for various industrial purposes.Key words: Indonesian poultry, lysozyme, egg, kampung chicken, Cihateup duck ABSTRAK Lisozim putih telur memiliki fungsi yang luas, salah satunya adalah memiliki aktivitas sebagai antibakteri terutama terhadap bakteri Gram positif. Lisozim dari putih telur �uga banyak diapli-. Lisozim dari putih telur �uga banyak diaplikasikan di industri pangan dan dinyatakan aman untuk pangan. Sampai saat ini lisozim dari putih telur yang berasal dari unggas lokal (Indonesia) belum dipela�ari dan dikembangkan. Tu�uan penelitian ini adalah melakukan purifikasi putih telur dari telur unggas lokal, yaitu ayam kampung dan itik Cihateup dibandingkan dengan telur ayam ras. Sampel telur yang didapatkan dari Laboratorium Lapang Fakultas Peternakan diklasifikasikan pada grade AA berdasarkan kualitas interior. Tahap pertama purifikasi lisozim putih telur dengan presipitasi etanol menghasilkan protein lain selain lisozim (purifikasi parsial). Konsentrasi lisozim putih telur hasil purifikasi parsial telur ayam kampung, ayam ras dan itik Cihateup masing-masing adalah 5800, 5400, dan 5500 ug/mL. Untuk melihat efek penggunaan etanol dalam proses purifikasi terhadap aktivitast antibakteri lisozim, kemampuan daya hambat lisozim terhadap Staphylococcus aureus telah dianalisis. Lisozim putih telur hasil pu...

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Section: Resultsmentioning

confidence: 99%

Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks

Wulandari

Fardiaz²,

Budiman³

et al. 2015

Med Pet

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“…Contudo, há referências de melhoria da qualidade da casca dos ovos, quando se empregam baixos níveis de fósforo (HOLDER, 1981). ROLAND (1986) estabelece comentários sobre as possíveis hipóteses envolvidas com a melhoria da qualidade da casca dos ovos, quando há redução dos níveis de fósforo, mas ampla revisão envolvendo vários estudos com este elemento foi realizada por ROLAND (1989).…”

Section: Introductionunclassified

Efeito de diferentes níveis de manganês e fósforo sobre o desempenho e a qualidade da casca dos ovos de poedeiras comerciais

et al. 1999

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RESUMO -O objetivo desta pesquisa foi avaliar o efeito de diferentes níveis de manganês e fósforo (P) sobre o desempenho e a qualidade da casca dos ovos e de determinados constituintes sangüíneos, em 96 galinhas Hy-Line W36, com 60 semanas de idade, distribuídas em 24 lotes com quatro aves cada. O delineamento usado foi o inteiramente ao acaso em arranjo fatorial (3x2), níveis de manganês (70, 140 e 210 ppm) e níveis de fósforo total (0,35 e 0,55%), totalizando seis tratamentos com quatro repetições cada. As rações experimentais foram isoprotéicas (16,0% de PB), isocalóricas (2800 kcal de EM/kg), isocálcicas (4,0% de Ca) e isosódicas (0,16% de Na). Os níveis de manganês não alteraram a produção e a qualidade da casca dos ovos. No entanto, quando as galinhas receberam as rações contendo 0,35% de P total, foi observado decréscimo na produção, no peso e na massa de ovos, sem alterações nas características de qualidade da casca dos ovos. Os níveis suplementares de manganês não foram efetivos em melhorar a qualidade da casca dos ovos de poedeiras na fase final de produção. O nível de 0,35% de P total não foi adequado para as aves expressarem as características normais de produção.Palavras-chave: fósforo, manganês, nutrição, poedeiras, qualidade da casca dos ovos Effect of Different Levels of Manganese and Phosphorus on the Performance and Eggshell Quality of Laying HensABSTRACT -The objective of this experiment was to evaluate the different levels of manganese and phosphorus (P) on the performance and eggshell quality and on determined blood constituents of 96 laying hens Hy-Line W36, with 60 weeks of age, distributed in 24 lots with four hens each. A completely randomized experimental design was used in a factorial arrangement (3 x 2), manganese levels (70, 140 and 210 ppm) and total phosphorus levels (.35 and .55%), totalizing six treatments with four replicates each. The experimental diets were isoproteic (16.0% CP), isocaloric (2800 kcal ME/kg), isocalcium (4.0% Ca) and isosodium (.16% Na). The manganese levels did not alter the production and eggshell quality. However, when hens were fed diets containing .35% of total P, there were observed decreases in the egg production, egg weight and egg mass, without alterations in eggshell quality characteristics. The supplemental manganese levels were not effective to improve eggshell quality at the final phase of production. The total phosphorus level of .35% was inadequate for the laying hens to express the normal production characteristics

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“…300-400 mg/day) by the hens enables satisfactory laying rate. However, basing on the results of many experiments, Roland (1986) considers that the intake of P by hens should be higher, reaching about 700 mg/hen/day at the peak of laying and between 500 and 600 mg later on. This assumption seems to confirm the results obtained by Keshavarz (1986) who found that, depending on P intake from the diet (540 -880 mg), the utilization of P ranged from 21.1 to 30.5%.…”

Section: Phosphorus Balancementioning

confidence: 99%

Effect of dietary phosphorus level and supplemental phytase on performance of Hisex Brown laying hens and egg shell quality

Kaminska

Skraba²,

Koreleski³

1996

J. Anim. Feed Sci.

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The experiment was carried out on Hisex Brown hens aged 26-48 weeks divided into 12 groups of 18 birds kept in single cages. Layers were fed wheat-maize-soyabean meal diets containing 0.50,0.55 and 0.60% total phosphorus (TP), 0.25, 0.30 and 0.35 % available phosphorus (AP) supplemented with 0,150, 300 or 450 units of microbial phytase (FTU) per kg. Hens were fed diet containing 0.50% TP supplemented with 300 FTU showed the highest laying rate (93%) and good egg shell quality.However, the thickest egg shells and the highest breaking strenght were found when diets containing 0.30-0.35 % AP were supplemented with 150 FTU. Hens fed diet with 0.25% AP + 300 FTU excreted 100 mg/day/hen less P than those fed diet with 0.35 % AP (0.60% TP), not supplemented with phytase. As the optimal levels in the diet are considered 0.25 % AP + 300 FTU or 0.30 % AP + 150 FTU.

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Egg Shell Quality III: Calcium and phosphorus requirements of commercial Leghorns

Cited by 46 publications

References 54 publications

Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks

Purification of Egg White Lysozyme from Indonesian Kampung Chicken and Ducks

Efeito de diferentes níveis de manganês e fósforo sobre o desempenho e a qualidade da casca dos ovos de poedeiras comerciais

Effect of dietary phosphorus level and supplemental phytase on performance of Hisex Brown laying hens and egg shell quality

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