Egg quality criteria in Pacific red snapper (Lutjanus peru)

Abstract

The aim of this study was to evaluate the biochemical parameters used as possible determinants of egg quality in Pacific red snapper (Lutjanus peru). Fertilized eggs of eight spawns were obtained by hormonal induction. Egg quality criteria, including abnormal cleavage (AC), hatching percentage (HR) and survival percentage at first feeding (SR) were recorded. Samples were taken during embryonic development and from yolk‐sac larvae. Proteins, energetic metabolite concentrations and metabolic and digestive enzyme activities were determined using colorimetric methods. Pearson's correlation, and simple and multiple regression models were performed using the biochemical parameters as the independent variables and AC, HR and SR as the dependant variables. Glucose‐6‐phosphatase activity (AC r = 0.87; HR r = −0.65; SR r = −0.67) and fructose concentration (AC r = −0.64; HR r = 0.54; SR r = 0.64) were the only biochemical parameters to be strongly correlated with the three egg quality criteria. The use of multiple regression models increased the regression coefficient of the three quality criteria. Fructose, glucose and glucose‐6‐phosphatase were involved in all multiple regression models. The models proposed in this study may be used to explain egg quality for Pacific red snapper and their use as predictors of egg quality is discussed.

Organogenesis of the digestive system in Pacific red snapper (Lutjanus peru ) larvae

“…Final maturation was hormonally induced with LHRH‐a at 25 mg kg −1 of fish, and gametes were collected by manual stripping, following the method reported by Moguel‐Hernández et al . (). Eggs were placed in a 120 L cylinder‐conical incubation tank filled with filtered (1 μm) and chlorine‐sterilized seawater, with constant flow‐through (1 L min −1 ) and gentle bottom aeration at 26°C.…”

“…In recent years, there has been an increasing interest from both the private and public sectors in developing a sustainable culture programme for this species as a viable economic alternative in the region. Research on this species initially focused on broodstock maturation and spawning in captivity (Dumas, Rosales‐Velázquez, Contreras‐Olguín, Hernández‐Ceballos & Silverberg ; Pelcastre‐Campos ), and some aspects of egg quality have also been reported recently (Moguel‐Hernández, Peña, Nolasco‐Soria, Dumas & Hinojosa‐Baltazar ). However, larviculture trials of Pacific red snapper have been characterized by very low survival at first feeding, with most trials leading to total mortality during the first week of development.…”

Evaluacion de la primera madurez sexual del huachinango del Pacifico (Lutjanus peru) nacido en cautiverio

“…Además, existen estudios con esta misma especie donde se reportan porcentajes de eclosión de hasta 95% a partir de desoves obtenidos de manera natural en peces silvestres (Estrada-Godínez et al, 2015); aunque cuando se trata de desoves obtenidos por inducción hormonal los porcentajes de eclosión larval disminuyen significativamente a 88% (Pintos-Terán et al, 2003) y hasta 70% (Moguel-Hernández et al, 2013). El máximo porcentaje de eclosión observado en este estudio alcanzó a 85%, a pesar de ser desoves obtenidos de manera natural, atribuible a las condiciones de cautiverio o bien por tratarse de la primera temporada reproductiva de los individuos (Carrillo et al, 2009) o por un deficiente aporte nutricional en la dieta, que en muchos casos, se ha observado que influye fuertemente en la calidad de los desoves (Izquierdo et al, 2001).…”

“…Sin embargo, al igual que muchas otras especies con potencial de cultivo, presenta disfunciones reproductivas que impiden que se realice con éxito su reproducción en cautiverio. Una alternativa para la obtención de desoves, ha sido la inducción hormonal para lograr la maduración final de los ovocitos y la espermiación Pintos-Terán et al, 2003;Moguel-Hernández et al, 2013), aunque con estos métodos se ha reportado una pobre calidad en los desoves, y bajas tasas de supervivencia en las etapas tempranas de desarrollo larvario (Phelps, 2003;Mylonas et al, 2010), no se ha reportado, la obtención de desoves de manera natural en cautiverio.…”

Timing of Puberty and Reproductive Performance in Wild-Reared and Hatchery-Reared Pacific Red Snapper Lutjanus peru

“…The effect of increasing water temperatures on egg and larvae quality remains unknown. However, methods to increase survival during larval metamorphosis in Pacific red snapper are being studied (Zavala-Leal et al, 2013;Moguel-Hernández et al, 2015).…”