EGFR protein overexpression correlates with chromosome 7 polysomy and poor prognostic parameters in clear cell renal cell carcinoma

Đorđević, Gordana; Matušan-Ilijaš, Koviljka; Hadžisejdić, Ita; Maričić, Anton; Grahovac, Blaženka; Jonjić, Nives

doi:10.1186/1423-0127-19-40

Cited by 35 publications

(22 citation statements)

References 27 publications

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“…40 Overexpression of EGFR in RCC is correlated with cell proliferation, high tumor stage, high nuclear grade, tumor recurrence, and overall survival. 41 PD-L1 and EGFR expression in RCC. 8 Therefore, the present study showed that claspin might be a promising molecule for treating RCC.…”

Section: Discussionmentioning

confidence: 98%

“…Epidermal growth factor receptor plays a significant role in promoting tumor progression, and its associated signaling pathways are well established targets in cancer therapy . Overexpression of EGFR in RCC is correlated with cell proliferation, high tumor stage, high nuclear grade, tumor recurrence, and overall survival . Thus, EGFR was considered a key factor in the prognosis of RCC and a potential target for the treatment of RCC.…”

Section: Discussionmentioning

confidence: 99%

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Claspin overexpression is associated with high‐grade histology and poor prognosis in renal cell carcinoma

et al. 2020

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Renal cell carcinoma (RCC) is one of the most common human cancers. We previously reported that claspin is a key regulator in the progression of gastric cancer, and it likely plays an important role in cancer stem cells of gastric cancer. However, the significance of claspin in RCC has not been examined. First, we analyzed the expression and distribution of claspin in 95 RCC cases by immunohistochemistry. In the nonneoplastic kidney, the staining of claspin was either weak or absent, whereas RCC tissue showed nuclear staining. In total, claspin expression was detected in 45 (47%) of 95 RCC cases. The claspin staining appeared relatively stronger in high nuclear grade RCC than in low nuclear grade RCC. Claspin‐positive RCC cases were associated with higher T grade, tumor stage, nuclear grade, vein invasion, and poorer prognosis. CLSPN siRNA treatment decreased RCC cell proliferation. The levels of phosphorylated Erk and Akt were lower in CLSPN siRNA‐transfected RCC cells than in control cells. In addition, claspin was coexpressed with CD44, epidermal growth factor receptor, p53, and programmed death ligand‐1. These results suggest that claspin plays an important role in tumor progression in RCC and might be a prognostic marker and novel therapeutic target molecule.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Claspin overexpression is associated with high‐grade histology and poor prognosis in renal cell carcinoma

et al. 2020

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“…And in model systems of autocrine stimulation of EGFR, or EGFR overexpression and mutation, consequent transformation has been observed [26, 27]. EGFR may function through activating a network of signaling pathways, involving Ras, phosphoinositide 3-kinase and the signal transducer, activator of transcription family [28-30], and so forth in ccRCC [31, 32]. …”

Section: Discussionmentioning

confidence: 99%

LINC00037 Inhibits Proliferation of Renal Cell Carcinoma Cells in an Epidermal Growth Factor Receptor-Dependent Way

Gong

et al. 2018

Cell Physiol Biochem

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Background/Aims: LINC00037 has previously been reported to be up-regulated in clear cell renal cell carcinoma (ccRCC), however, the underlying mechanism remained unknown. In this study, we designed to investigate the functional role of LINC00037 in ccRCC Methods: LINC00037 knockdown and re-expressing 786-O and A498 cells were established. CCK8 assay and EdU assay were performed to evaluate the proliferation rates of ccRCC cells. Flow cytometry assay was performed to detect the cell apoptosis and cell cycle. Subcutaneous injection xenotransplantation mouse model was used to observe the role of LINC00037 in tumor growth in vivo. Mass spectrometry (MS) was performed to find the interacting partner of LINC00037 and RNA immunoprecipitation (RIP) was carried out to validate their interaction. Results: We found that knockdown of LINC00037 resulted in inhibited cell proliferation with activated apoptosis and cell cycle arrest in vitro. Over-expression of LINC00037 in LINC00037 knockdown cells restored and enhanced cell proliferation. In vivo mouse model indicated reduced tumor progression by LINC00037 depletion and promoted tumor progression by LINC00037 overexpression. LINC00037 could bind to epidermal growth factor receptor (EGFR) and increase the protein level of EGFR. Conclusion: LINC00037 could inhibit proliferation of ccRCC in an epidermal growth factor receptor-dependent way.

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“…Liang et al reported the inhibitory effect of silibinin on EGFR signal-induced renal cell carcinoma progression via suppression of the EGFR/MMP-9 signaling pathway [4]. The effect of EGR on renal cell carcinoma was also reported by others [5, 6]. EGFR activation is required to induce the renal fibrotic genes [7, 8].…”

Section: Introductionmentioning

confidence: 91%

Sex difference in EGFR pathways in mouse kidney-potential impact on the immune system

Liu

Jiao

et al. 2016

BMC Genet

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BackgroundEpidermal growth factor receptor (Egfr) has been the target of several drugs for cancers. The potential gender differences in genes in the Egfr axis have been suggested in humans and in animal models. Female and male mice from the same recombinant inbred (RI) strain have the same genomic components except the sex difference. A population of different RI mouse strains allows to conduct precise analysis of molecular pathways and regulation of Egfr between female and male mice.MethodsThe whole genome expression profiles of 70 genetically diverse RI strains of mice were used to compare three major molecular aspects of Egfr gene: the relative expression levels, gene network and expression quantitative trait loci (eQTL) that regulate the expression of Egfr between female and male mice.ResultsOur data showed that there is a significant sex difference in the expression levels in kidney. A considerable number of genes in the gene network of Egfr are sex differentially expressed. The expression levels of Egfr in mice are statistical significant different between C57BL/6 J (B6) and DBA/2 J (D2) genotypes in male while no difference in female mice. The eQTLs that regulate the expression levels of Egfr between female and male mice are also different. Furthermore, the differential expression levels of Egfr showed significantly different correlations with two known biological traits between male and female mice.ConclusionOverall there is a substantial sex difference in the Egfr pathways in mice. These data may have significant impact on drug target design, development, formulation, and dosage determinant for women and men in clinical trials.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-016-0449-3) contains supplementary material, which is available to authorized users.

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EGFR protein overexpression correlates with chromosome 7 polysomy and poor prognostic parameters in clear cell renal cell carcinoma

Cited by 35 publications

References 27 publications

Claspin overexpression is associated with high‐grade histology and poor prognosis in renal cell carcinoma

Claspin overexpression is associated with high‐grade histology and poor prognosis in renal cell carcinoma

LINC00037 Inhibits Proliferation of Renal Cell Carcinoma Cells in an Epidermal Growth Factor Receptor-Dependent Way

Sex difference in EGFR pathways in mouse kidney-potential impact on the immune system

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