LINC00037 Inhibits Proliferation of Renal Cell Carcinoma Cells in an Epidermal Growth Factor Receptor-Dependent Way

Abstract

Background/Aims: LINC00037 has previously been reported to be up-regulated in clear cell renal cell carcinoma (ccRCC), however, the underlying mechanism remained unknown. In this study, we designed to investigate the functional role of LINC00037 in ccRCC Methods: LINC00037 knockdown and re-expressing 786-O and A498 cells were established. CCK8 assay and EdU assay were performed to evaluate the proliferation rates of ccRCC cells. Flow cytometry assay was performed to detect the cell apoptosis and cell cycle. Subcutaneous injection xenotransplantation mouse model was used to observe the role of LINC00037 in tumor growth in vivo. Mass spectrometry (MS) was performed to find the interacting partner of LINC00037 and RNA immunoprecipitation (RIP) was carried out to validate their interaction. Results: We found that knockdown of LINC00037 resulted in inhibited cell proliferation with activated apoptosis and cell cycle arrest in vitro. Over-expression of LINC00037 in LINC00037 knockdown cells restored and enhanced cell proliferation. In vivo mouse model indicated reduced tumor progression by LINC00037 depletion and promoted tumor progression by LINC00037 overexpression. LINC00037 could bind to epidermal growth factor receptor (EGFR) and increase the protein level of EGFR. Conclusion: LINC00037 could inhibit proliferation of ccRCC in an epidermal growth factor receptor-dependent way.

Proteogenomics analysis of non-coding region encoded peptides in normal tissues and five cancer types

“…For examples, the peptides encoded by noncoding gene DGCR5 showed significantly higher expression level in CCRCC compared to adjacent normal tissues ( Figure 3c). This contradicts the finding at RNA level where DGCR5 is ubiquitously expressed in normal tissues 11 . In Uterine Corpus Endometrial Carcinoma (UCEC), peptides detected from 5' UTR or non-canonical reading frame of protein coding genes, TSGA10, NPLOC4, MKKS and MUC1 were more abundant in tumors compared to normal tissues (Figure 3d).…”

“…For example, the non-coding RNA genes DGCR5 (DiGeorge Syndrome Critical Region Gene 5, located on chromosome 22q11), also known as LINC00037, were detected with ten unique peptides in clear cell renal cell carcinoma (CCRCC). Previous study by Gong et al reported DGCR5 is ubiquitously expressed in normal tissues, with much higher expression in CCRCC compared to normal kidney tissues 11 . Our analysis showed that DGCR5 probably encodes a functional protein product in CCRCC.…”

Expression of POT1-AS1 in GC Tissue, Its Effect on Biological Behavior of Gastric Cancer, and Its Significance on Prognosis of Gastric Cancer

“…ncRNA can be classified according to its length because of its different length. When ncRNA <200 nt is called shortchain ncRNA, ncRNA >200 nt, it is called long-chain ncRNA [25]. Neither long-chain ncRNA nor short-chain ncRNA can encode protein due to lack of ability to encode protein [26].…”

lncRNA ZEB1-AS1 promotes migration and metastasis of bladder cancer cells by post-transcriptional activation of ZEB1

“…A total of 25 µ g proteins in each lane were loaded for SDS-PAGE (10% gel) at 4°C. The concentrating voltage was 80 V and separating voltage was 110 V. The binding proteins were also identified by mass spectrometry to identify the potential binding proteins (H. Wayen Biotechnology, Shanghai, China) as previously described (21).…”