EGFR inhibition by (-)-epigallocatechin-3-gallate and IIF treatments reduces breast cancer cell invasion

Farabegoli, Fulvia; Govoni, Marzia; Spisni, Enzo; Papi, Alessio

doi:10.1042/bsr20170168

Cited by 25 publications

(24 citation statements)

References 45 publications

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“…The study suggests that MFC-7 cells are susceptible to EGCG in the context of suppression of the migration process. This is also the basis for confirming the correctness of the results obtained in this work [15]. Another research study proved that polyphenol extract from Phyllanthus emblica (PEEP) tan grass leaves obtained by extraction with 70% acetone, inhibited proliferation of HeLa cells by 39% at a dose of PEEP 150 mg/mL [16].…”

Section: Resultssupporting

confidence: 85%

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

Zakłos‐Szyda

Pawlik

2019

Acta Innovations

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In recent years, research of antitumor activity of natural compounds isolated from plant material has increased. Polyphenols have gained significant attention due to their proapoptotic abilities and their involvement in migration and inhibition of metastasis processes. The anticancer effects of polyphenolic extracts of Viburnum opulus fruit against human breast (MCF-7) and cervical (HeLa) cancer cell lines have been confirmed in this study. It was demonstrated that the tested preparations (methanol – M and acetone – A from pomace, juice – J and juice after extraction to the solid phase SPE – PF) show cytotoxic activity and regulate the migration process of cancer cells. The degree of inhibition of cell migration was measured at two times - 24 h and 48 h after addition of the tested preparations. The highest toxicity towards both cell lines was demonstrated by the polyphenol fraction obtained after juice purification SPE (IC50 values at concentration of 63,541 and 19,380 μg/mL for HeLa and MCF cell lines, respectively). At the same time, the same preparation inhibited cell migration the most (nearly 70% compared to controls at both times at the concertation of 15 and 30 μg/mL). All preparations showed the antioxidant ability, but the Viburnum opulus juice (200 and 350 g/mL) and the preparation after its purification (15 and 30 μg/mL) have larger ability to inhibit the intracellular oxidative stress (30-40%) than preparation obtained from pomace (nearly by 20% at concentration of 20 and 50 μg/mL of M and A). Despite the antioxidative capacity of the preparations, they simultaneously decreased cellular mitochondrial potential. The results obtained indicate the high potential of components of Viburnum opulus polyphenolic compounds can be used in the production of innovative dietary supplements or pharmacological preparations for people with an increased risk or inclination towards developing breast or cervical cancer.

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Section: Resultssupporting

confidence: 85%

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

Zakłos‐Szyda

Pawlik

2019

Acta Innovations

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“…Among these two gelatinases, MMP2 and MMP9, are often upregulated and strongly associated with breast cancer (42). Reduction of MMP2 and MMP9 levels was revealed to suppress growth and invasion of MCF-7 breast cancer cells (43)(44)(45)(46). We determined that at a non-toxic dose (75 µM), terrein significantly reduced the number of invading cells in both cell lines, but to a lesser degree in MCF-7 cells.…”

Section: Discussionmentioning

confidence: 90%

Terrein inhibits migration of human breast cancer cells via inhibition of the Rho and Rac signaling pathways

et al. 2018

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Breast cancer is the most common cancer in women worldwide. Progression and aggressiveness of breast cancer is usually associated with its migration and invasion abilities. Recently, natural products with potential anticancer activity have become attractive candidates for alternative treatment of cancer. A fungal metabolite, terrein, isolated from the Aspergillus terreus has been revealed to exhibit selective anticancer activity; although this molecule has a variety of biological activities. The inhibitory effect on cell proliferation in hepatoma, keratinocytes, and lung cancer cells was due to cell cycle arrest without induction of apoptosis. In contrast, its effects on cervical and breast cancer cells were mediated through activation of the apoptotic process. However, the effect of terrein on cell migration and invasion has not been explored. In the present study we analyzed the molecular effects of terrein on cell adhesion, cell migration, and cell invasion using two breast cancer cell lines, MCF-7 and MDA-MB-231, which exhibit different levels of invasiveness. Terrein induced apoptosis in both breast cancer cell lines in a dose-dependent manner. In addition, at a non-toxic concentration terrein exhibited a weak inhibition of cell adhesion, using either fibronectin or type IV collagen as substrates. Notably, terrein significantly inhibited both the migration and invasion abilities of MDA-MB-231 cells at the same non-toxic concentration. A marked decrease in MMP-2 and MMP-9 transcripts, as evaluated by real-time PCR, confirmed the anti-invasion effect of terrein at the transcriptional level. Western blot analyses revealed that terrein treatment suppressed RhoB expression and reduced Rac1 phosphorylation, leading to Rho GTPase inhibition. In addition, terrein-treated MCF-7 and MDA-MB-231 cells both displayed a scattered pattern of migration, suggesting that the suppression of RhoB and Rac1 disturbed the collective migration processes of breast cancer cells.

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“…Amplification or uncontrolled activation of EGFR signaling has been implicated in the invasion and metastasis of many cancers. In breast cancer cells, inhibition of EGFR signaling reduces breast cancer cell invasion [ 24 ]. CCR1 is involved in the invasion and metastasis of several cancer types [ 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

C-C motif chemokine receptor 1 (CCR1) is a target of the EGF-AKT-mTOR-STAT3 signaling axis in breast cancer cells

Shin

Lee

et al. 2017

Oncotarget

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The CC motif chemokine receptor 1 (CCR1) has been implicated in tumor invasion and metastasis in numerous cancers. However, the detailed mechanism of CCR1 upregulation in metastatic tumor cells is poorly understood. The aim of this study was to clarify the regulatory mechanism underlying transcriptional activation of the CCR1 gene in response to epidermal growth factor (EGF) stimulation in breast cancer cells. CCR1 was highly expressed in human breast invasive ductal carcinoma (IDC) compared to adjacent normal tissues. Upon EGF stimulation, CCR1 expression was upregulated at the transcriptional level. Promoter analysis showed that signal transducer and activator of transcription 3 (STAT3) is necessary for EGF-induced CCR1 promoter activation, and STAT3 silencing abrogated EGF-induced CCR1 transcription. Pharmacological inhibition and short hairpin RNA-mediated knockdown experiments showed that AKT-dependent mammalian target of rapamycin (mTOR) activation was involved in the phosphorylation of serine-727 of STAT3, which in turn stimulated the transcription of the CCR1 gene. In conclusion, the AKT-mTOR-STAT3 signaling axis contributes to EGF-induced CCR1 expression, which promotes invasion and metastasis in breast cancer cells. We propose that the AKT-mTOR-STAT3 axis is a potential therapeutic target for blocking the invasion and metastasis of breast cancers.

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EGFR inhibition by (-)-epigallocatechin-3-gallate and IIF treatments reduces breast cancer cell invasion

Cited by 25 publications

References 45 publications

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

Terrein inhibits migration of human breast cancer cells via inhibition of the Rho and Rac signaling pathways

C-C motif chemokine receptor 1 (CCR1) is a target of the EGF-AKT-mTOR-STAT3 signaling axis in breast cancer cells

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