EGFR fluorescence in situ hybridisation assay: guidelines for application to non-small-cell lung cancer

Varella‐Garcia, Marileila; Diebold, Joachim; Eberhard, David A.; Geenen, K; Hirschmann, Astrid; Kockx, Mark; Nagelmeier, Iris; Rüschoff, J.; Schmitt, Martina; Arbogast, Stefanie; Cappuzzo, Federico

doi:10.1136/jcp.2009.066548

Cited by 108 publications

(95 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Positive FISH cases showed high polysomy or amplification and the same procedure was followed to HER-2 probe (HER-2/Neu (17q12)/SE17; Kreatech diagnostics; Amsterdam). Positive and negative FISH cases were according to Varella-Garcia et al 21 The microscopic analyses were done in a Nikon Eclipse 80i of brilliant field and epifluorescent microscope (LUCIA cytogenetics software). Images were captured and registered with a digital camera (Nikon DXM 1220F), in monochromatic images/layers posterior joint in one single image.…”

Section: Fluorescent In Situ Hybridization ---Fishmentioning

confidence: 99%

Lung adenocarcinoma: Sustained subtyping with immunohistochemistry and EGFR, HER2 and KRAS mutational status

Sousa

Rodrigues

Silva

et al. 2015

Revista Portuguesa de Pneumologia (English Edition)

View full text Add to dashboard Cite

Pulmonary adenocarcinomas are still in the process of achieving morphological, immunohistochemical and genetic standardization. The ATS/ERS/IASLC proposed classification for lung adenocarcinomas supports the value of the identification of histological patterns, specifically in biopsies.Thirty pulmonary adenocarcinomas were subjected to immunohistochemical study (CK7, CK5, 6, 18, CK20, TTF1, CD56, HER2, EGFR and Ki-67), FISH and PCR followed by sequencing and fragment analysis for EGFR, HER2 and KRAS.Solid pattern showed lower TTF1 and higher Ki-67 expression. TTF1 expression was higher in non-mucinous lepidic and micropapillary patterns when compared to acinar and solid and acinar, solid and mucinous respectively. Higher Ki67 expression was present in lepidic and solid patterns compared to mucinous. EGFR membranous staining had increasing expression from non-mucinous lepidic/BA pattern to solid pattern and micropapillary until acinar pattern. EGFR mutations, mainly in exon 19, were more frequent in females, together with non-smoking status, while KRAS exon 2 mutations were statistically more frequent in males, especially in solid pattern. FISH EGFR copy was correlated gross, with mutations. HER2 copy number was raised in female tumours without mutations, in all cases. Although EGFR and KRAS mutations are generally considered mutually exclusive, in rare cases they can coexist as it happened in one of this series, and was represented in acinar pattern with rates of 42.9% and 17.9%, respectively. EGFR mutations were more frequent in lepidic/BA and acinar patterns. Some cases showed different EGFR mutations.The differences identified between the adenocarcinoma patterns reinforce the need to carefully identify the patterns present, with implications in diagnosis and in pathogenic understanding. EGFR and KRAS mutational status can be determined in biopsies representing bronchial

show abstract

Section: Fluorescent In Situ Hybridization ---Fishmentioning

confidence: 99%

Lung adenocarcinoma: Sustained subtyping with immunohistochemistry and EGFR, HER2 and KRAS mutational status

Sousa

Rodrigues

Silva

et al. 2015

Revista Portuguesa de Pneumologia (English Edition)

View full text Add to dashboard Cite

show abstract

“…Analysis of GCN alterations in MST1R and MET were performed and cut-off points were applied as previously described for MET, EGFR and IGF1R genes. 45,47,80 Interpretation of HER2/CEP17 FISH was performed as previously described for GEC. 7,73 To our knowledge, the cut off points for MET FISH-positivity has not yet been standardized; FISH studies of MST1R have not yet being reported.…”

Section: Methodsmentioning

confidence: 99%

RON(MST1R)is a novel prognostic marker and therapeutic target for gastroesophageal adenocarcinoma

Catenacci

Cervantes

Yala

et al. 2011

Cancer Biology & Therapy

107

View full text Add to dashboard Cite

“…The criteria for the EGFR FISH enumeration were adapted from Varella-Garcia et al 27 (Z15/100), the sample was considered to be positive. These parameters are consistent with methods used in studies examining both EGFR and ALK in lung adenocarcinoma.…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

“…These parameters are consistent with methods used in studies examining both EGFR and ALK in lung adenocarcinoma. 13,[27][28][29] …”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

EGFR alterations and EML4-ALK rearrangement in primary adenocarcinoma of the urinary bladder

et al. 2014

View full text Add to dashboard Cite

The identification of mutations in epidermal growth factor receptor (EGFR) and translocations involving anaplastic lymphoma kinase (ALK) in lung adenocarcinoma has drastically changed understanding of the disease and led to the development of targeted therapies. Adenocarcinoma of the urinary bladder is rare and poorly understood at the molecular level. We undertook this study to determine whether EGFR mutations, increases in EGFR copy number, or ALK translocations are present in these tumors. Twenty-eight cases of primary bladder adenocarcinoma were analyzed. For EGFR mutational analysis, PCR-amplified products were analyzed on the Q24 Pyrosequencer with Qiagen EGFR Pyro Kits. All cases were analyzed via fluorescence in situ hybridization (FISH) using Vysis ALK Break Apart FISH Probes for detection of ALK chromosomal translocation and Vysis Dual Color Probes to assess for increased gene copy number of EGFR. None of the 28 cases examined showed mutational events in EGFR or ALK rearrangements. EGFR polysomy was seen in 10 out of 28 (36%) cases. No correlation with EGFR polysomy was seen in the tumors with respect to age, histologic subtypes, pathologic stage, or lymph node metastasis. In summary, EGFR mutations and ALK rearrangements do not appear to be involved in the development of primary adenocarcinoma of the urinary bladder. A subgroup of cases (36%), however, demonstrated increased gene copy number of EGFR by FISH.

show abstract

EGFR fluorescence in situ hybridisation assay: guidelines for application to non-small-cell lung cancer

Cited by 108 publications

References 30 publications

Lung adenocarcinoma: Sustained subtyping with immunohistochemistry and EGFR, HER2 and KRAS mutational status

Lung adenocarcinoma: Sustained subtyping with immunohistochemistry and EGFR, HER2 and KRAS mutational status

RON(MST1R)is a novel prognostic marker and therapeutic target for gastroesophageal adenocarcinoma

EGFR alterations and EML4-ALK rearrangement in primary adenocarcinoma of the urinary bladder

Contact Info

Product

Resources

About