EGCG attenuates the neurotoxicity of methylglyoxal via regulating MAPK and the downstream signaling pathways and inhibiting advanced glycation end products formation

Yin, He; Yang, Zhijun; Pi, Jingwen; Cai, Tiange; Xia, Ying; Cao, Xiangyu; Liu, Jianli

doi:10.1016/j.foodchem.2022.132358

Cited by 24 publications

(12 citation statements)

References 36 publications

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“…We then indirectly verified our findings in LPS-stimulated macrophages in vitro to examine the suppression effects of EGCG on the LPS-like responses in COVID-19. Finally, our study is slightly different from the abovementioned literature (61)(62)(63)(64)(65)(66)(67) in terms of molecular signaling pathways. Based on the results of the bioinformatics analysis, we focused on the most credible MAPK (ERK1/2-JNK-P38) signaling pathway.…”

Section: Discussioncontrasting

confidence: 58%

“…Through the LPS-stimulated macrophage model, we attempted to demonstrate the possibility of EGCG molecules indirectly inhibiting COVID-19 inflammation. EGCG has been reported to alleviate acute lung injury, regulate the polarization of macrophages to M2 (61), and inhibit secretion of inflammatory factors, and its protective mechanism may be related to the inhibition of the MAPK and NF-κB signaling pathway (62)(63)(64). In addition, EGCG derivatives have anti-inflammatory activity in LPS-stimulated mouse macrophages (65).…”

Section: Discussionmentioning

confidence: 99%

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Section: Discussioncontrasting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

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“…In the presence of nerve growth factor (NGF), PC12 cells differentiate into sympathetic ganglion neurons and exhibit some features of mature dopaminergic neurons but lose the ability to reproduce (Sergi et al, 2021). In absence of NGF, PC12 cells maintain mitotic activity in culture, allowing for assessments of the effects of bioactive compounds on cell growth (He et al, 2022). The present studies explored the effects of lutein on cell survival and apoptosis in response to MGO‐induced oxidative stress using a PC12 cells model.…”

Section: Discussionmentioning

confidence: 99%

Lutein attenuated methylglyoxal‐induced oxidative damage and apoptosis in PC12 cells via the PI3K /Akt signaling pathway

Chen

Zhang

Liu

et al. 2022

Journal of Food Biochemistry

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Methylglyoxal (MGO), a cytotoxic byproduct of glycolysis, causes neuro oxidative damage and apoptosis, and plays key roles in diabetic encephalopathy (DE). The goal of this research was to evaluate the roles of lutein attenuated MGO‐induced damage in PC12 cells as well as the underlying mechanisms. The findings of this study showed that lutein has a significant impact on reducing the generation of reactive oxygen species (ROS) and oxidative stress in MGO‐induced PC12 cells, which may be attributed to the increased antioxidant enzymes activity and the decreased MDA levels. Moreover, treatment with lutein also alleviated cell apoptosis and mitochondrial damage. Real‐time PCR and western blot analysis showed that lutein enhanced the Bcl‐2:Bax ratio, inhibited the expression of caspase‐3 and caspase‐9, and increased the protein level of phosphorylated Akt. The network pharmacology and molecular docking prediction results suggested that the phosphoinositide 3‐kinase/protein kinase B (PI3K/Akt) signaling pathway was a potential mechanism of lutein in DE treatment. Furthermore, LY294002, a specific PI3K inhibitor, partially abolished the protective effect of lutein. These results presented that lutein attenuated oxidative damage and apoptosis triggered by MGO in PC12 cells via the PI3K/Akt signaling pathway. Practical applications Lutein is a common carotenoid dispersed in fruits and vegetables. This article confirmed a protective effect of lutein on oxidative damage and apoptosis in PC12 cells after MGO damage. These results indicated that lutein could potentially be developed as a nutraceutical or functional food in the prevention of diabetic‐related neurodegenerative diseases.

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“…EGCG has been reported to alleviate acute lung injury, regulate the polarization of macrophages to M2 ( 61 ), and inhibit secretion of inflammatory factors, and its protective mechanism may be related to the inhibition of the MAPK and NF-κB signaling pathway ( 62 – 64 ). In addition, EGCG derivatives have anti-inflammatory activity in LPS-stimulated mouse macrophages ( 65 ).…”

Section: Discussionmentioning

confidence: 99%

Tea Ingredients Have Anti-coronavirus Disease 2019 (COVID-19) Targets Based on Bioinformatics Analyses and Pharmacological Effects on LPS-Stimulated Macrophages

et al. 2022

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Coronavirus disease 2019 (COVID-19) is a contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that caused millions of deaths and lacks treatment. Although several studies have focused on the major component of green tea, epigallocatechin 3-gallate (EGCG), which is efficient in preventing COVID-19, systemic analyses of the anti-COVID-19 potential of green tea remain insufficient. Here, we co-analyzed the target genes of tea ingredients and COVID-19 signature genes and found that epigallocatechin 3-acetalbehyde was capable of reversing the major molecular processes of COVID-19 (MAPK and NF-κB activation). These findings were further supported by Western blotting (WB), immunofluorescence, and quantitative polymerase chain reaction (qPCR) in LPS-stimulated macrophages. Moreover, using molecular docking analysis, we identified three tea ingredients ((-)-catechin gallate, D-(+)-cellobiose, and EGCG) that may interact with the vital SARS-CoV-2 protein, 5R84, compared with the qualified 5R84 ligand WGS. Thus, our results indicated that tea ingredients have the potential to treat COVID-19 by suppressing the COVID-19 signature genes and interacting with the vital SARS-CoV-2 protein.

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EGCG attenuates the neurotoxicity of methylglyoxal via regulating MAPK and the downstream signaling pathways and inhibiting advanced glycation end products formation

Cited by 24 publications

References 36 publications

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Lutein attenuated methylglyoxal‐induced oxidative damage and apoptosis in PC12 cells via the PI3K /Akt signaling pathway

Tea Ingredients Have Anti-coronavirus Disease 2019 (COVID-19) Targets Based on Bioinformatics Analyses and Pharmacological Effects on LPS-Stimulated Macrophages

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