Efficient virus-induced gene silencing in Cynodon dactylon and Zoysia japonica using rice tungro bacilliform virus vectors

Biotechnology & Biotechnological Equipment

Liang

2017

Bermudagrass is widely distributed as a warm-season turf and forage grass in the temperate and tropical zones around the world. Its strong vegetative reproduction and ability to withstand drought make it an ideal species as a most used warm-season forage grass and turfgrass. Genetic transformation is an important tool for the study of gene function and for germplasm improvement in bermudagrass. This paper attempts to present a recent review on genetic resources, plant regeneration and genetic transformation in bermudagrass. We first review the various genetic resources and collection of bermudagrass. Then the explants, basal medium and the effect of different cultivars and plant growth regulators on plant regeneration in bermudagrass are also summarized. Last, we outline the main areas of progress in genetic transformation with either the biolistic or Agrobacterium-mediated method in bermudagrass, and discuss various factors that influence Agrobacterium-mediated transformation. However, the question that still remains is why there have been no genetic modification reports on bermudagrass for 10 years.

Section: Prospectsmentioning

confidence: 99%

Section: Prospectsmentioning

confidence: 99%

Genetic resources and genetic transformation in bermudagrass – a review

Huang

Biotechnology & Biotechnological Equipment

Liang

2017

“…The most cost-efficient and effective method of inoculating plants with virus-based vectors is agroinfection (Grimsley et al, 1986), but its efficiency varies among plants (Zhang et al, 2016). In turf grass, the silencing efficiency of the RTBV-VIGS system in Cynodon dactylon was such that 65.8%- 72.5% of the agroinfected plants developed symptoms typical for phytoene desaturase gene silencing, while the silencing efficiency in Zoysia japonica was much lower, with only 52.7%–55% of agroinfected plants developing the phenotype (Zhang et al, 2016). The ability of the TRV vector to directly infect woody plant species has been tested, and TRV-mediated VIGS was effective in Vernicia fordii , weak in Populus tomentosa Carr., and ineffective in Camellia oleifera (Jiang et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using tobacco rattle virus

et al. 2019

PeerJ

Background Hibiscus hamabo Sieb. et Zucc. is a semi-mangrove plant used for the ecological restoration of saline-alkali land, coastal afforestation and urban landscaping. The genetic transformation H. hamabo is currently inefficient and laborious, restricting gene functional studies on this species. In plants, virus-induced gene silencing provides a pathway to rapidly and effectively create targeted gene knockouts for gene functional studies. Methods In this study, we tested the efficiency of a tobacco rattle virus vector in silencing the cloroplastos alterados 1 (CLA1) gene through agroinfiltration. Results The leaves of H. hamabo showed white streaks typical of CLA1 gene silencing three weeks after agroinfiltration. In agroinfiltrated H. hamabo plants, the CLA1 expression levels in leaves with white streaks were all significantly lower than those in leaves from mock-infected and control plants. Conclusions The system presented here can efficiently silence genes in H. hamabo and may be a powerful tool for large-scale reverse-genetic analyses of gene functions in H. hamabo.

“…The most cost-efficient and effective method of inoculating plants with virus-based vectors is agroinfection (Grimsley et al 1986), but its efficiency varies among plants (Zhang et al 2016). In turf grass, the silencing efficiency of the RTBV-VIGS system in Cynodon dactylonis such that 65.8-72.5% of the agroinfected plants developed symptoms typical for phytoene desaturase gene silencing, while the silencing efficiency in Zoysia japonica is much weaker, with only 52.7%-55% of agroinfected plants developing the phenotype (Zhang et al 2016). The ability of the TRV vector to directly infect woody plant species has been tested, and TRV-mediated VIGS was effective in Vernicia fordii, weak in Populus tomentosa Carr., and ineffective in Camellia oleifera (Jiang et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using Tobacco rattle virus

et al. 2019

Preprint

Background. Hibiscus hamabo Sieb. et Zucc. is a semi-mangrove plant used for the ecological restoration of saline-alkali land, coastal afforestation and urban landscaping. Because the genetic transformation of H. hamabo is difficult, molecular breeding and gene functional studies have been severely restricted. In plants, virus-induced gene silencing provides a pathway to rapidly and effectively create targeted gene knockouts for gene functional studies. Methods. In this study, we tested the efficiency of a Tobacco rattle virus vector in silencing the cloroplastos alterados 1 (CLA1) gene through agroinfiltration. Results. The leaves of H. hamabo showed white streaks typical of CLA1 gene silencing three weeks after agroinfiltration. In agroinfiltrated H. hamabo plants, the CLA1 expression levels in leaves with white streaks were all significantly lower than in those of mock-infected and control plants. Conclusions. Thus, this virus-induced gene silencing system was efficient in H. hamabo and may be a powerful tool for large-scale reverse-genetic analyses of gene functions in H. hamabo.