“…The most cost-efficient and effective method of inoculating plants with virus-based vectors is agroinfection (Grimsley et al, 1986), but its efficiency varies among plants (Zhang et al, 2016). In turf grass, the silencing efficiency of the RTBV-VIGS system in Cynodon dactylon was such that 65.8%- 72.5% of the agroinfected plants developed symptoms typical for phytoene desaturase gene silencing, while the silencing efficiency in Zoysia japonica was much lower, with only 52.7%–55% of agroinfected plants developing the phenotype (Zhang et al, 2016). The ability of the TRV vector to directly infect woody plant species has been tested, and TRV-mediated VIGS was effective in Vernicia fordii , weak in Populus tomentosa Carr., and ineffective in Camellia oleifera (Jiang et al, 2014).…”