Efficient virus-induced gene silencing in <i>Hibiscus hamabo</i> Sieb. et Zucc. using tobacco rattle virus

Wang, Zhiquan; Xu, Xiaoyang; Ni, Lei; Guo, Jinbo; Gu, Chao

doi:10.7717/peerj.7505

Cited by 10 publications

(5 citation statements)

References 27 publications

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“…H. hamabo is a semi-mangrove plant that can survive in tidal saline soil and under immersion [ 7 , 8 ]. How it adapts to such stressful environmental conditions is an intriguing question.…”

Section: Introductionmentioning

confidence: 99%

The genome of Hibiscus hamabo reveals its adaptation to saline and waterlogged habitat

Wang

Xue

et al. 2022

Horticulture Research

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Hibiscus hamabo is a semi-mangrove species with strong tolerance to salt and waterlogging stress. However, the molecular basis and mechanisms that underlie this strong adaptability to harsh environments remain poorly understood. Here, we assembled a high-quality, chromosome-level genome of this semi-mangrove plant and analyzed its transcriptome under different stress treatments to reveal regulatory responses and mechanisms. Our analyses suggested that H. hamabo has undergone two recent successive polyploidy events, a whole-genome duplication followed by a whole-genome triplication, resulting in an unusually large gene number (107,309 genes). Comparison of the H. hamabo genome with that of its close relative Hibiscus cannabinus, which has not experienced a recent WGT, indicated that genes associated with high stress resistance have been preferentially preserved in the H. hamabo genome, suggesting an underlying association between polyploidy and stronger stress resistance. Transcriptomic data indicated that genes in the roots and leaves responded differently to stress. In roots, genes that regulate ion channels involved in biosynthetic and metabolic processes responded quickly to adjust the ion concentration and provide metabolic products to protect root cells, whereas no such rapid response was observed from genes in leaves. Using co-expression networks, potential stress resistance genes were identified for use in future functional investigations. The genome sequence, along with several transcriptome datasets, provide insights into genome evolution and the mechanism of salt and waterlogging tolerance in H. hamabo, suggesting the importance of polyploidization for environmental adaptation.

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“…H. hamabo is a semi-mangrove plant that can survive in tidal saline soil and under immersion [ 7 , 8 ]. How it adapts to such stressful environmental conditions is an intriguing question.…”

Section: Introductionmentioning

confidence: 99%

The genome of Hibiscus hamabo reveals its adaptation to saline and waterlogged habitat

Wang

Xue

et al. 2022

Horticulture Research

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“…Hibiscus hamabo Siebold & Zuccarini (Malvaceae) is naturally distributed in coastal sands near sea level in China, Japan and Korea and is cultivated in India and the Pacific islands [15]. As a semi-mangrove plant, H. hamabo has high tolerance to tidal saline soil, immersion and arid environments and is considered to be one of the best choices in increasing the vegetation in higher salinity landscapes [16,17]. Although there are several studies on the physiological responses of H. hamabo to salinity and drought [15,16], the molecular mechanisms underlying its stress tolerance remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals Regulatory Framework for Salt and Drought Tolerance in Hibiscus hamabo Siebold & Zuccarini

Wang

Hua

et al. 2021

Forests

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Hibiscus hamabo Siebold & Zuccarini (H. hamabo) is tolerant to salt and drought conditions, but the molecular mechanisms that underlie this stress tolerance remain unclear. In this study, the transcriptome of H. hamabo roots was investigated under NaCl or PEG treatment. A total of 20,513 and 27,516 significantly changed known genes at 6 h and 24 h, respectively, were detected between the salt or drought treatments and the control libraries. Among these, there were 3845 and 7430 overlapping genes under the two stresses at 6 h and 24 h, respectively. Based on the analysis of enriched KEGG pathways and clustering of expression patterns, the DEGs that were continuously up- or down-regulated under both salt and drought treatments were mainly enriched in MAPK signaling pathway, transcription factors, transporters and other pathways. The transcriptome expression profiles of H. hamabo provide a genetic resource for identifying common regulatory factors involved in responses to different abiotic stresses. In addition, the identified factors may be useful to developing genetic breeding strategies for the Malvaceae.

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“…Changed genes in mulberry or Arabidopsis both cause abnormal growth in plants. Furthermore, due to the albino phenotype and high conservation in plant species [ 46 , 47 ], CLA1 has currently been identified as a visual marker gene for VIGS in various plants, such as Arabidopsis [ 48 ], cotton [ 49 ], and Hibiscus hamabo [ 46 ]. Our study indicated that MaCLA1 can also be used as a marker for application in gene functional studies in mulberry.…”

Section: Discussionmentioning

confidence: 99%

Screening and Verification of Photosynthesis and Chloroplast-Related Genes in Mulberry by Comparative RNA-Seq and Virus-Induced Gene Silencing

Cui

et al. 2022

IJMS

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Photosynthesis is one of the most important factors in mulberry growth and production. To study the photosynthetic regulatory network of mulberry we sequenced the transcriptomes of two high-yielding (E1 and E2) and one low-yielding (H32) mulberry genotypes at two-time points (10:00 and 12:00). Re-annotation of the mulberry genome based on the transcriptome sequencing data identified 22,664 high-quality protein-coding genes with a BUSCO-assessed completeness of 93.4%. A total of 6587 differentially expressed genes (DEGs) were obtained in the transcriptome analysis. Functional annotation and enrichment revealed 142 out of 6587 genes involved in the photosynthetic pathway and chloroplast development. Moreover, 3 out of 142 genes were further examined using the VIGS technique; the leaves of MaCLA1- and MaTHIC-silenced plants were markedly yellowed or even white, and the leaves of MaPKP2-silenced plants showed a wrinkled appearance. The expression levels of the ensiled plants were reduced, and the levels of chlorophyll b and total chlorophyll were lower than those of the control plants. Co-expression analysis showed that MaCLA1 was co-expressed with CHUP1 and YSL3; MaTHIC was co-expressed with MaHSP70, MaFLN1, and MaEMB2794; MaPKP2 was mainly co-expressed with GH9B7, GH3.1, and EDA9. Protein interaction network prediction revealed that MaCLA1 was associated with RPE, TRA2, GPS1, and DXR proteins; MaTHIC was associated with TH1, PUR5, BIO2, and THI1; MaPKP2 was associated with ENOC, LOS2, and PGI1. This study offers a useful resource for further investigation of the molecular mechanisms involved in mulberry photosynthesis and preliminary insight into the regulatory network of photosynthesis.

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Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using tobacco rattle virus

Cited by 10 publications

References 27 publications

The genome of Hibiscus hamabo reveals its adaptation to saline and waterlogged habitat

The genome of Hibiscus hamabo reveals its adaptation to saline and waterlogged habitat

Transcriptome Analysis Reveals Regulatory Framework for Salt and Drought Tolerance in Hibiscus hamabo Siebold & Zuccarini

Screening and Verification of Photosynthesis and Chloroplast-Related Genes in Mulberry by Comparative RNA-Seq and Virus-Induced Gene Silencing

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