Efficient transformation and regeneration of carnation cultivars usingAgrobacterium

Firoozabady, E.; Moy, York; Tucker, William T.; Robinson, K. E. P.; Gutterson, Neal

doi:10.1007/bf02277428

Cited by 27 publications

(17 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The binary vectors (Figure 1) pALS1301, pNPT0402, pPO7022b, pPO7123 and pPO7127 were introduced into A. tumefaciens disarmed strain C58pmp90 (Koncz and Schell, 1986). Also, EHA101 (Hood et al, 1984) carrying plasmid pWTT2084 (Firoozabady et al, 1995) was used in some experiments.…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

“…The vector pALS1301 contains the Smas promoter (Ni et al, 1995) driving the GUS gene (Jefferson et al, 1987) and ubiquitin 1 promoter (Christiensen et al, 1992) driving the surB gene (Firoozabady et al, 1995), which confers CS resistance in the plant cells. The GUS gene contains a potato intron (Vancanneyt et al, 1990) to abrogate its expression in the bacteria and to verify its expression in the plant cells.…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

“…Recombinant DNA procedures were done essentially as described (Firoozabady et al, 1995). The binary vectors (Figure 1) pALS1301, pNPT0402, pPO7022b, pPO7123 and pPO7127 were introduced into A. tumefaciens disarmed strain C58pmp90 (Koncz and Schell, 1986).…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

See 2 more Smart Citations

Transformation and regeneration of pineapple

Firoozabady

Heckert

Gutterson

2005

Plant Cell Tiss Organ Cult

Self Cite

View full text Add to dashboard Cite

We have developed efficient methods for plant regeneration, via both organogenesis and embryogenesis, of Smooth Cayenne pineapple, Ananas comosus (L.) Merr. A range of different types of embryogenic tissues has been developed with varying properties in terms of growth rate and state of development (Firoozabady and Moy, 2004). Two of the embryogenic systems, namely friable embryogenic cell clusters (ECCs) and chunky non-dispersible embryogenic tissues (ETs) have been used for transformation of pineapple. The tissues were cocultivated for 2-3 days with Agrobacterium tumefaciens disarmed strain C58 carrying a binary vector containing either surB gene conferring resistance to chlorsulfuron or the nptII gene conferring resistance to geneticin (G418). After cocultivation and a recovery period, tissues were selected on media containing chlorsulfuron or G418. On average, about 50 or 120 independent transgenic lines were obtained from each gram of ECCs or ETs, respectively, inoculated with Agrobacterium. Transformed embryogenic tissues were transferred to maturation media to form somatic embryos, which subsequently produced transgenic pineapple plants. Transformation has been confirmed by GUS assay, polymerase chain reaction, and by Southern hybridization. Thousands of plants from independently transformed lines were transferred to the greenhouse and to the field to evaluate clonal fidelity and somaclonal variation.

show abstract

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

See 1 more Smart Citation

Transformation and regeneration of pineapple

Firoozabady

Heckert

Gutterson

2005

Plant Cell Tiss Organ Cult

Self Cite

View full text Add to dashboard Cite

show abstract

“…Riviera Rose, Riviera White, and Riviera Midnight Blue, respectively. Such high frequencies have not been reported in other ornamental plants- Kishimoto et al (2002) obtained 3.2% for begonia, Firoozabady et al (1995) obtained 7.1% for carnation, and Ledger et al (1997) obtained 3.3% for lisianthus. The high transformation frequency of L. erinus makes this system substantially more useful for molecular studies than other flowering plants.…”

Section: Transformation With the Direct Shoot Regeneration Systemmentioning

confidence: 54%

High-frequency transformation of Lobelia erinus L. by Agrobacterium -mediated gene transfer

2004

View full text Add to dashboard Cite

A highly efficient transformation procedure was developed for Lobelia erinus. Leaf or cotyledon discs were inoculated with Agrobacterium tumefaciens strain EHA105 harboring the binary vector plasmid pIG121Hm, which contains a beta-glucuronidase gene with an intron as a reporter gene and both the neomycin phosphotransferase II and hygromycin phosphotransferase genes as selectable markers. The hygromycin-resistant calli produced on the selection medium were transferred to MS medium supplemented with 0.5 mg/l benzyladenine and 0.2 mg/l indole-3-acetic acid for regeneration of adventitious shoots. Transgenic plants were obtained as a result of the high regeneration rate of the transformed calli, which was as high as 83%. In contrast, no transgenic plant was obtained by the procedure of direct shoot formation following inoculation with A. tumefaciens. Transgenic plants flowered 3-4 months after transformation. Integration of the transgenes was detected using PCR and Southern blot analysis, which revealed that one to several copies were integrated into the genomes of the host plants. The transformation frequency at the stage of whole plants was very high--45% per inoculated disc.

show abstract

“…This procedure was named the conventional method. Briefly, leaf explants, pulled off from shoot clusters of the shoot culture, were mixed with Agrobacterium tumefaciens EHA 101 cells in Minimal A medium (Lech and Brent 1992) supplemented with 100 lM acetosyringone for 15 min, and cocultivated in BD medium (Firoozabady et al 1995), which contained 6-benzylaminopurine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) as plant hormones, for a week at 24°C in the dark. The concentrations of BA and 2,4-D were 0.49 lM and 0.57 lM, respectively, in the BD medium and those of 3-indoleacetic acid and thidiazuron in the IT medium for re-differentiation were 0.44 lM and 0.45 lM, respectively.…”

Section: Transformationmentioning

confidence: 99%

Transformation of carnation with genes related to ethylene production and perception: towards generation of potted carnations with a longer display time

Kinouchi

Endo

Yamashita

et al. 2006

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Potted carnation (Dianthus caryophyllus L. cv. Lillipot) plants were transformed with cDNAs for carnation 1-aminocyclopropane-1-carboxylate (ACC) synthase (DC-ACS1, s/aACS transgenes) or ACC oxidase (DC-ACO1, s/aACO transgenes) in sense or antisense orientation or mutated carnation ethylene receptor cDNA (DC-ERS2¢) by Agrobacterium-mediated gene transfer. The presence of acetosyringone at 100 lM in media for shoot culture prior to leaf explant preparation and preculture of Agrobacterium in addition to the conventional method of addition to media for infection and coculture, and the use of water instead of nutrient media for infection and coculture increased the transformation efficiency to 4.0% compared to the 0.1% obtained by the conventional method. PCR analysis as well as Southern blot analysis confirmed the integration of the ethylene-related transgenes. Leaflet segments of cultured shoots of some lines transformed with s/aACO transgenes had less activity to convert ACC to ethylene than that of the non-transformed control plant, indicating that the integrated s/aACO transgenes reduced the expression of endogenous ACC oxidase gene (DC-ACO1) in the cultured shoots.

show abstract

Efficient transformation and regeneration of carnation cultivars usingAgrobacterium

Cited by 27 publications

References 36 publications

Transformation and regeneration of pineapple

Transformation and regeneration of pineapple

High-frequency transformation of Lobelia erinus L. by Agrobacterium -mediated gene transfer

Transformation of carnation with genes related to ethylene production and perception: towards generation of potted carnations with a longer display time

Contact Info

Product

Resources

About