Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9

Du, Hongyang; Zeng, Xuanrui; Zhao, Meng; Cui, Xiaopei; Wang, Qing; Yang, Hui; Cheng, Hao; Yu, Deyue

doi:10.1016/j.jbiotec.2015.11.005

Cited by 216 publications

(114 citation statements)

References 48 publications

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“…This technology has already been optimized in three model legume crops, soybean (Sun et al, 2015;Li et al, 2015), Lotus japonicas and Medicago truncatula (Meng et al, 2017). The efficiency of genome editing using various endogenous and exogenous RNA polymerase III promoters has been compared (Sun et al, 2015;Du et al, 2016) and in soybean the Cas9-single guide RNA together with donor DNA fragments was successfully transformed into soybean using the particle bombardment method (Li et al, 2015). Medicago and Lotus, having smaller diploid genomes, are the model legume crops for conducting research in several areas including symbiotic nitrogen fixation.…”

Section: Genome Editingmentioning

confidence: 99%

Accelerating genetic gains in legumes for the development of prosperous smallholder agriculture: integrating genomics, phenotyping, systems modelling and agronomy

Varshney

Thudi

Pandey

et al. 2018

Journal of Experimental Botany

100

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Grain legumes form an important component of the human diet, provide feed for livestock, and replenish soil fertility through biological nitrogen fixation. Globally, the demand for food legumes is increasing as they complement cereals in protein requirements and possess a high percentage of digestible protein. Climate change has enhanced the frequency and intensity of drought stress, posing serious production constraints, especially in rainfed regions where most legumes are produced. Genetic improvement of legumes, like other crops, is mostly based on pedigree and performance-based selection over the past half century. To achieve faster genetic gains in legumes in rainfed conditions, this review proposes the integration of modern genomics approaches, high throughput phenomics, and simulation modelling in support of crop improvement that leads to improved varieties that perform with appropriate agronomy. Selection intensity, generation interval, and improved operational efficiencies in breeding are expected to further enhance the genetic gain in experimental plots. Improved seed access to farmers, combined with appropriate agronomic packages in farmers' fields, will deliver higher genetic gains. Enhanced genetic gains, including not only productivity but also nutritional and market traits, will increase the profitability of farming and the availability of affordable nutritious food especially in developing countries.

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Section: Genome Editingmentioning

confidence: 99%

Accelerating genetic gains in legumes for the development of prosperous smallholder agriculture: integrating genomics, phenotyping, systems modelling and agronomy

Varshney

Thudi

Pandey

et al. 2018

Journal of Experimental Botany

100

View full text Add to dashboard Cite

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“…The Golden Gate method (Addgene) has been used for assembling multiple DNA fragments in an ordered fashion via custom repeat arrays in about three days and has enabled the targeting of genomic sites in rice [31]. We applied the FastTALE™ TALEN assembly kit (Sidansai Biotechnology) for Marchantia, which has been employed to generate an efficient targeted mutagenesis in soybean [32]. This kit offers the advantage that TALEN repeat arrays can be constructed by a one-step assembly and ligation procedure in only half a day.…”

Section: Resultsmentioning

confidence: 99%

TALEN-mediated genome-editing approaches in the liverwort Marchantia polymorpha yield high efficiencies for targeted mutagenesis

et al. 2017

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BackgroundThe liverwort Marchantia polymorpha occupies a crucial position in land plant evolution and provides the opportunity to investigate adaptations to a terrestrial plant life style. Marchantia reverse genetic analyses have thus far been conducted by employing a homologous recombination approach, which yields an efficiency of around 3%. Availability of the characterized and suitable endogenous MpEF1α promoter prompted us to establish the TALEN gene targeting technique for Marchantia.ResultsHere, two different TALEN techniques, using custom and self-assembled TALEN constructs, were applied and compared. The MpNOP1 gene was selected as a candidate gene, as the respective knockout mutant has been shown to lack air chamber formation, representing an easily traceable phenotype. We demonstrate that both TALEN approaches are successful in Marchantia yielding high gene targeting efficiencies of over 20%. Investigation of selected G1 up to G4 generations proved the stability of the knockout mutants. In 392 analyzed T1 plants, no additional phenotypes were observed and only one chimeric knockout plant was detected after an extended cultivation period. Interestingly, two out of the 24 sequenced mutants harbored indels causing in-frame mutations and revealed novel Mpnop1-related phenotypes. This demonstrates the potential to detect crucial amino acids and motives of targeted proteins, which is of special interest for essential genes where full knockouts are lethal. The FastTALE™ TALEN assembly kit enables the rapid assembly and ligation of the TALEN arms within half a day. For transformations, custom and assembled constructs were subcloned into Marchantia binary vectors possessing the MpEF1α promoter.ConclusionConsidering time, costs and practicability, the assembly TALEN approach represents a rapid and highly efficient gene targeting system to generate Marchantia knockout mutants, which can be further adapted for future advanced genome-editing applications.

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“…Additionally, the application of CRISPR/Cas systems in maize (Z. mays) seems to be mainly studied in the U.S.A. Efficient systems for genome editing in soybean have also been reported for example [61]. Other crops that were studied include vegetables and industrial plants:…”

Section: Geographic Distribution Of Studied Articlesmentioning

confidence: 99%

Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture

Ricroch

Clairand

Harwood

2017

Emerging Topics in Life Sciences

193

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Initially discovered in bacteria and archaea, CRISPR-Cas9 is an adaptive immune system found in prokaryotes. In 2012, scientists found a way to use it as a genome editing tool. In 2013, its application in plants was successfully achieved. This breakthrough has opened up many new opportunities for researchers, including the opportunity to gain a better understanding of plant biological systems more quickly. The present study reviews agricultural applications related to the use of CRISPR systems in plants from 52 peerreviewed articles published since 2014. Based on this literature review, the main use of CRISPR systems is to achieve improved yield performance, biofortification, biotic and abiotic stress tolerance, with rice (Oryza sativa) being the most studied crop.

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Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9

Cited by 216 publications

References 48 publications

Accelerating genetic gains in legumes for the development of prosperous smallholder agriculture: integrating genomics, phenotyping, systems modelling and agronomy

Accelerating genetic gains in legumes for the development of prosperous smallholder agriculture: integrating genomics, phenotyping, systems modelling and agronomy

TALEN-mediated genome-editing approaches in the liverwort Marchantia polymorpha yield high efficiencies for targeted mutagenesis

Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture

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