Efficient Somatic Embryogenesis and Plantlet Regeneration From Protoplast Culture of Crocus Sativus L.

Karamian, Roya; Ranjbar, Massoud

doi:10.17660/actahortic.2010.850.17

Cited by 11 publications

(11 citation statements)

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“…While, the corm explants cultured on MS medium supplemented with 1 mg L -1 NAA plus 1 mg L -1 BAP or Kin and 4 mg L -1 2,4-D plus 0.5 mg L -1 Kin exhibited lowest percentage of callus induction and callus yield in without sonication condition (Figures 2 and 3). In the study of Vahedi et al (2015) callus formation from saffron was successfully achieved by using a combination of 2.4-D and Kinetin hormones, which agreed with studies reported by Chaloushi et al (2007), Karamian & Ranjbar (2010) and Dalila et al (2013). They observed the highest frequency of callus induction (44%) on MS medium supplemented with 2.4-D (2 mg L -1 ) and Kinetin (0.5 mg L -1 ), culture from apical meristem.…”

supporting

confidence: 87%

In Vitro Indirect Somatic Embryogenesis and Secondary Metabolites Production in the Saffron: Emphasis on Ultrasound and Plant Growth Regulators

Firoozi¹,

Zare²,

Sofalian³

et al. 2019

Tarım Bilimleri Dergisi

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In the present study, the effects of ultrasound and plant growth regulators on in vitro callogenesis and secondary metabolites production in saffron calli were investigated. Accordingly, the saffron corms surface sterilized, sonicated and cultured on different concentrations of plant growth regulators (0.5, 1, 2 and 4 mg L-1 2,4-D or NAA in combination with 0.5 and 1 mg L-1 Kin or BAP). The percentage of callus induction, callus yield (fresh weight) and embryogenic callus formation were recorded and secondary metabolites of calli were measured by UV/VIS spectrophotometer three months after culture. The results indicated that sonication of the saffron corm explants significantly increased the in vitro callus induction and growth. So, the highest callus induction (100%) and yield (4.68 g) was achieved with sonicated explants cultured on MS medium supplemented with 2 mg L-1 1-naphthaleneacetic acid (NAA) and 0.5 mg L-1 kinetin (Kin). Somatic embryogenesis was significantly influenced by plant growth regulator regimes and the MS medium supplemented with 0.5 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) plus 0.5 mg L-1 6-benzylaminopurine (BAP) and 0.5 mg L-1 NAA plus 0.5 mg L-1 Kin exhibited the highest percentage (75 and 72, respectively) of somatic embryogenesis. Secondary metabolite content of the callus cells was significantly different among the plant growth regulator regimes and the highest production of picrocrocin and safranal were occurred on the medium containing 0.5 mg L-1 2,4-D plus 0.5 mg L-1 BAP and 1 mg L-1 NAA plus 1 mg L-1 BAP.

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supporting

confidence: 87%

In Vitro Indirect Somatic Embryogenesis and Secondary Metabolites Production in the Saffron: Emphasis on Ultrasound and Plant Growth Regulators

Firoozi¹,

Zare²,

Sofalian³

et al. 2019

Tarım Bilimleri Dergisi

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“…This is consistent with a previous study [54] , where germinated embryos were transferred to half-strength MS medium supplemented with BAP and NAA. Regeneration via somatic embryogenesis has been derived from corm-derived callus cultures [18] , [32] , [55] .…”

Section: Discussionmentioning

confidence: 99%

Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species

Verma

Das

Cingoz

et al. 2016

Biotechnology Reports

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HighlightsCrocus is a medicinally important plant and it is costliest spice of the world.An efficient microprapogation protocol of five Turkish Crocus species was developed.Crocus species: C. specious ssp. Specious, C. oliveri spp. Oliveri, C. pestalozzae, C. abantensis, and C. paschei..Different combinations and concentrations of auxins and cytokinins were used.Plant regeneration was developed via somatic embryogenesis.

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“…Protoplasts are the living material of a plant or bacterial cell, which include the protoplasm and plasma membrane after the cell wall is removed. Due to removal of the cell wall, plant protoplasts have been widely used for genetic transformation, cell fusion [5] and somatic mutation for generating unique and novel plants [3] , [6] , [7] , [8] . Protoplasts obtained through enzymatic cell wall digestion are highly vacuolated and fragile and are easy to rupture during the following experimental steps, such as washing, isolation, and transfection, making it difficult for long-term culture and monitoring of biological processes.…”

Section: Additional Informationmentioning

confidence: 99%

A simple and effective method to encapsulate tobacco mesophyll protoplasts to maintain cell viability

Lei¹,

Qiao²,

Hu³

et al. 2015

MethodsX

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Efficient Somatic Embryogenesis and Plantlet Regeneration From Protoplast Culture of Crocus Sativus L.

Cited by 11 publications

References 0 publications

In Vitro Indirect Somatic Embryogenesis and Secondary Metabolites Production in the Saffron: Emphasis on Ultrasound and Plant Growth Regulators

In Vitro Indirect Somatic Embryogenesis and Secondary Metabolites Production in the Saffron: Emphasis on Ultrasound and Plant Growth Regulators

Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species

A simple and effective method to encapsulate tobacco mesophyll protoplasts to maintain cell viability

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