Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species

Verma, Sandeep Kumar; Das, Ashok Kumar; Cingoz, Gunce Sahin; Uslu, Emel; Gürel, Ekrem

doi:10.1016/j.btre.2016.03.006

Cited by 65 publications

(47 citation statements)

References 46 publications

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“…As it turned out from our investigations, but also from the works of other authors (Ali & Iqbal, 2012;Bosila et al, 2003;Burbulis et al, 2007;Fatima et al, 2009;Polanco et al, 1988;Verma et al, 2016), the qualities of the callus obtained in in vitro culture (texture, colour, speed of cell proliferation, content in certain substances, etc. ), its subsequent evolution, biomass production, etc., depend on many factors, such as: the species, the genotype of the plant, the type of explant, its age, the composition of the nutrient medium, the growth regulators present in the culture medium (the type, concentration and ratio between them, in vitro cultivation conditions (light/dark, photoperiod, light quality and intensity, temperature) etc.…”

Section: Ocimum Basilicum -Primary Internode Callussupporting

confidence: 78%

A Journey into of the Universe of in vitro Cultures of Plants. Callogenesis

Ghiorghiţă¹

2019

ENRR

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After presenting a brief history of the discovery of this system of cultivation of plant tissues and cells, of the evolution in time of knowledge in this field, of the pathways of in vitro morphogenesis in plants, the paper focuses on in vitro callogenesis in plants. There are presented the types of callus that can be generated via in vitro culture (illustrated with aspects from the author's experience), the influence of factors related to the biological nature of the explants, the composition of the nutrient medium, the cultivation conditions, etc., upon some characteristics of the callus obtained. A special chapter is dedicated to the importance of in vitro cultures of callus in plants.

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Section: Ocimum Basilicum -Primary Internode Callussupporting

confidence: 78%

A Journey into of the Universe of in vitro Cultures of Plants. Callogenesis

Ghiorghiţă¹

2019

ENRR

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“…However the germination rates of other genotypes like American chestnut (3.3%), European chestnut (27.5%), as well as Chinese chestnuts, as seen from the result in this study (2.1%) were rather low (Xing et al 1999;Sezgin and Dumanoğlu 2014). This may be due to different hormone ratios and genotypes resulting in the changes of regeneration rates (Verma et al 2016). In addition, germinated shoots or roots were obtained from somatic embryos of Chinese chestnut, but whole plantlets directly conversed by somatic embryos was not observed.…”

Section: Germination Of Mature Embryoscontrasting

confidence: 37%

“…Germination difficulty of the embryos was a main limitation during somatic embryogenesis regeneration, and germination rates vary considerably in many plant species (Xing et al 1999;Odutayo et al 2005;Sezgin and Dumanoğlu 2014;Manoharan et al 2016). Some species expressed relatively high germination rates (>50%), such as Pinus densiflora, Lilium ledebourii and Turkish Crocus species (Bakhshaie et al 2010;Kim and Moon 2014;Verma et al 2016). However the germination rates of other genotypes like American chestnut (3.3%), European chestnut (27.5%), as well as Chinese chestnuts, as seen from the result in this study (2.1%) were rather low (Xing et al 1999;Sezgin and Dumanoğlu 2014).…”

Section: Germination Of Mature Embryosmentioning

confidence: 99%

Establishment of a somatic embryo regeneration system and expression analysis of somatic embryogenesis-related genes in Chinese chestnut (Castanea mollissima Blume)

Wei

Zhou

et al. 2017

Plant Cell Tiss Organ Cult

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embryos were germinated in embryo germination medium consisting of WPM with 0.5 μM NAA and 0.5 μM 6-BA, resulting in shoot regeneration with a 2.1% conversion rate. Additionally, eight embryogenesis-related genes were identified, and the expression profiles of these genes during embryogenesis were analyzed via quantitative real-time RT-PCR (qRT-PCR). The CmSERK, CmLEC1, CmWUS and CmAGL15 genes exhibited high expression in the initial embryo stages, which inferred that these genes played key roles during the initiation of embryogenesis. Studies on embryogenesis-related genes will provide an insight for further elucidating molecular mechanism during somatic embryogenesis of Chinese chestnut. Furthermore, the successful establishment of a somatic embryo regeneration system for Chinese chestnut will lay a significant foundation for a stable genetic transformation system and genetic improvement.Keywords Castanea mollissima · Somatic embryogenesis · Regeneration · Embryogenesis-related genes · Gene expression Abstract Somatic embryogenesis is a reliable and important tool, and the relevant genes controlling this process act as vital roles through the whole development of somatic embryos. However, regeneration via somatic embryogenesis in Chinese chestnut has been impeded and its molecular mechanism is not known. Therefore, firstly we described a protocol for somatic embryo initiation, development, maturation and germination. Embryogenic calli were obtained in embryo initiation medium containing 1.8 μM 2,4-D and 1.1 μM 6-BA, and then were transferred to embryo development medium without any hormones for at least 4 weeks, until cotyledonary embryos appeared. Next, the somatic embryos were transferred to embryo maturation medium containing Gamborg's B-5 Basal Salt Mixture with 0.5 μM NAA and 0.5 μM 6-BA for 3 weeks. Finally, these mature Communicated by: Jose M. Segui-Simarro.Dan Lu, Wei Wei and Wan Zhou have contributed equally to this work. Electronic supplementary materialThe online version of this article

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“…These culture conditions involve mainly somatic embryogenesis and include cultures initiated from corm explants: (i) decrease of PGR content in culture media together with changing of auxin/cytokinin ratio in favor of cytokinins and decrease of medium strength (Karamian, 2007;Demeter et al, 2010;Karamian and Ebrahimzadeh, 2010); (ii) high cytokinin content of media, with or without the use of auxin(s) (this strategy was used mainly for C. sativus and can be used for micropropagation purposes as well, where callus induction is not involved. It works well with both corm and shoot tip explants and can lead to cormlet formation, too -Devi et al, 2014); (iii) high organic carbon source (sucrose) content (up to 8%) with or without the use of IBA was reported to promote rooting of regenerated Crocus shoots and cormlet formation (Sharma et al, 2008;Verma et al, 2016). Besides, cormlet development in Crocus tissue cultures occurs under various PGR treatments.…”

Section: Discussionmentioning

confidence: 99%

“…This includes not only cultures initiated from explants of floral organs, but also for corm explants. For the induction of calli capable of plant regeneration, this rule should be taken into account (Bhagyalakshmi, 1999;Plessner and Ziv, 1999;Demeter et al, 2010;Verma et al, 2016). Indeed, nearly all Crocus cultures involved in this study were initiated on a medium with 10 mg L -1 NAA and 1 mg L -1 BA.…”

Section: Discussionmentioning

confidence: 99%

Production and Characterization of Tissue Cultures of Four Crocus Species from the Carpathian Basin

Freytag¹,

Pabar²,

Demeter³

et al. 2017

Acta Biologica Cracoviensia S. Botanica

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We aimed to produce tissue cultures and plant regeneration from endangered Crocus species: C. scepusiensis, C. tommasinianus, C. vittatus ("Verni" series of the genus) and C. banaticus. For initiation of cultures we used a plant growth regulator (PGR) combination used for in vitro culture of saffron and its relatives: 10 mg L -1 α-naphthaleneacetic acid (NAA) and 1 mg L -1 6-benzyladenine (BA). Shoot tips of young seedlings (C. scepusiensis) and corms (for the rest of species) were used as explants. C. scepusiensis explants developed into organogenic calli. On media with decreased NAA and with or without increased BA concentration, calli produced stigma-like structures and/or shoots and whole plants. In the other species, callus initiation medium induced callus formation with abundant somatic embryos. In C. tommasinianus, embryos developed shoots when auxin content of medium was decreased. In C. banaticus, a decrease of auxin with or without an increase in cytokinin content led to shoot or whole plant regeneration, as in C. scepusiensis. In the case of C. vittatus and C. banaticus, initiation and/or maintenance of cultures on indole-3-butyric acid (IBA) and increased sucrose concentration stimulated whole plant regeneration and in vitro cormlet development. C. scepusiensis and the rest of cultures (organogenic vs. embryogenic) differed at the biochemical level: C. scepusiensis cultures had higher (yet still low) enzymatic antioxidant (catalase, peroxidase) activities. With respect to catalase isoenzyme patterns, C. banaticus was different from the rest of cultures, demonstrating its distinct taxonomical position. Besides germplasm preservation use of the present cultures, they have a potential biotechnological value.

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Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species

Cited by 65 publications

References 46 publications

A Journey into of the Universe of in vitro Cultures of Plants. Callogenesis

A Journey into of the Universe of in vitro Cultures of Plants. Callogenesis

Establishment of a somatic embryo regeneration system and expression analysis of somatic embryogenesis-related genes in Chinese chestnut (Castanea mollissima Blume)

Production and Characterization of Tissue Cultures of Four Crocus Species from the Carpathian Basin

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