Efficient Separation of Phytochemicals from Muehlenbeckia volcanica (Benth.) Endl. by Polarity-Stepwise Elution Counter-Current Chromatography and Their Antioxidant, Antiglycation, and Aldose Reductase Inhibition Potentials

Abstract

Muehlenbeckia volcanica (Benth.) Endl. (M. volcanica), native to South America, is a traditional Peruvian medicinal plant that has multi-therapeutic properties; however, no phytochemicals have been identified from it yet. In this study, a five-step polarity-stepwise elution counter-current chromatography (CCC) was developed using methanol/water (1:5, v/v) as the stationary phase and different ratios of n-hexane, ethyl acetate, and n-butanol as mobile phases to separate the compounds from the 70% methanol extract of M. volcanica, by which six compounds with a wide range of polarities were separated in a single run of CCC and were identified as gallic acid, protocatechuic acid, 4,4′-dihydroxy-3,3′-imino-di-benzoic acid, rutin, quercitrin, and quercetin. Then, two compounds from the fractions of stepwise elution CCC were separated using conventional high-speed CCC, pH-zone-refining CCC, and preparative high-performance liquid chromatography, and identified as shikimic acid and miquelianin. These compounds are reported from M. volcanica for the first time. Notably, except for shikimic acid, all other compounds showed anti-diabetic potentials via antioxidant, antiglycation, and aldose reductase inhibition. The results suggest that the polarity-stepwise elution CCC can be used to efficiently separate or fractionate compounds with a wide range of polarities from natural products. Moreover, M. volcanica and its bioactive compounds are potent anti-diabetic agents.

Screening and Evaluation of Active Compounds in Polyphenol Mixtures by a Novel AAPH Offline HPLC Method and Its Application

“…While investigating other factors, this study used 75 °C, 30 min, and 500 µmol to control for variables. Trolox content was quantified using a standard curve (50–500 µmol) and detected at 291 nm using a UV spectrophotometer (UV1601, SHIMADZU, Kyoto, Japan) [ 15 ].…”

Separation and Identification of Antioxidants and Aldose Reductase Inhibitors in Lepechinia meyenii (Walp.) Epling

“…The preparation of rat lens AR and AR inhibition assay were conducted as we previously reported [11]. The eyes of 10-week Sprague-Dawley rats (250-280 g) were removed and kept at −70 • C before use.…”

“…The infusion of L. meyenii is used as a traditional herb medicine in Peru to treat diabetes, cough, inflammation, diarrhea, spasm, burning sensation in the stomach, and pain in the stomach and joints [8][9][10]. In our ongoing research to screen and isolate potential anti-diabetic agents from natural products [11][12][13][14], we found that the 70% MeOH extract of L. meyenii has strong antioxidant and AR inhibitory activities [15], indicating the presence of potent antioxidants and AR inhibitors in this plant. Many diterpenoids have been previously identified from L. meyenii.…”

Cuantificación de Antocianinas y Compuestos Fenólicos en Muehlenbeckia Volcanica (Bentham) Endlicher (Mullaca)-Recuay, Ancash

“…La creciente demanda mundial por productos naturales con propiedades antioxidantes, ha vuelto la mirada a la búsqueda de especies vegetales nativas, con alto contenido de antocianinas y compuestos fenólicos, capaces de reducir los radicales libres y disminuir el riesgo de enfermedades relacionadas al estrés oxidativo, y a los se les ha atribuido entre otros, actividad antiinflamatoria, antimicrobiana (Santos et al, 2014), anticancerígena (Cai et al, 2004;Garzón, 2008) y antidiabética (Wang et al, 2017;Zuo et al, 2021).…”

“…7714 (2008) que valida su potencial utilidad antiinflamatoria. A nivel internacional, Wang et al (2017) han reportado que, el extracto crudo de M. volcánica muestra una inhibición relativamente alta (65,92%) de la enzima aldosa-reductasa (AR) y posee notable actividad antioxidante confirmada por el ensayo DPPH (94,74%) y el ABTS (98,892%); recientemente Zuo et al (2021), utilizando cromatografía a contracorriente han logrado por primera vez separar e identificar seis compuestos bioactivos de hojas de M. volcánica (Ac.…”