Efficient screening for ‘genetic pollution’ in an anthropogenic crested newt hybrid zone

Wielstra, Ben; Burke, Terry; Butlin, Roger K.; Schaap, Onno; Shaffer, H. Bradley; Vrieling, Klaas; Arntzen, Jan W.

doi:10.1007/s12686-016-0582-3

Cited by 10 publications

(16 citation statements)

References 30 publications

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“…For instance, Taillebois et al, ( 2020 ) developed 77 species‐specific SNPs (single nucleotide polymorphisms) that could detect hybrids and backcrosses between allis shad ( Alosa alosa ) and twaite shad ( A. fallax ) up to the third generation. Similar marker sets and sequencing protocols have been developed for other study systems (Feulner & Seehausen, 2019 ; Vaux et al, 2021 ; Wielstra et al, 2016 ) and show that conservationists do not always need whole genome sequencing data to reconstruct the entire genomic landscape. This conclusion is further supported by evolutionary studies that used less powerful sequencing methods (e.g., RADseq or ultraconserved elements) to explore the genomic landscape of differentiation (Battey, 2019 ; Bourgeois et al, 2020 ; Oswald et al, 2019 ; Plomion et al, 2018 ).…”

Section: Development Of Molecular Markersmentioning

confidence: 93%

“…This concept assumed that species differentiation is controlled by a large number of genetic loci and that the whole genome functions as an integrated and cohesive genetic unit. Hybridization and consequent genetic exchange were thought to destroy this integrity and break up “co‐adapted gene complexes.” This perspective is still widely followed by conservationists that describe introgressive hybridization as “genetic erosion” (Chafin et al, 2019 ) or “genetic pollution” (Wielstra et al, 2016 ). However, genomic studies have overturned the idea of the genome as a cohesive genetic unit that can be destroyed by hybridization.…”

Section: The Genic View Of Speciation and Introgressionmentioning

confidence: 99%

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The genic view of hybridization in the Anthropocene

Ottenburghs

2021

Evolutionary Applications

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Section: Development Of Molecular Markersmentioning

confidence: 93%

Section: The Genic View Of Speciation and Introgressionmentioning

confidence: 99%

The genic view of hybridization in the Anthropocene

Ottenburghs

2021

Evolutionary Applications

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“…Labels indicate collapsed ND2 + ND4 haplotypes from Canestrelli et al (2012). Triturus newts commonly hybridize in natural (Arntzen, Wielstra, & Wallis, 2014;Maletzky et al, 2008) and invasive ranges, which contributes to the spread of T. carnifex genes (Brede, 2015;Dufresnes et al, 2016;Meilink, Arntzen, van Delft, & Wielstra, 2015;Wielstra et al, 2016). Triturus cristatus was used as outgroup nearby, or hobbyists/uninformed naturalists (Dubey et al, 2019).…”

Section: Emerging Invasion Of Italian Crested Newtsmentioning

confidence: 99%

“…Second, we documented limited genetic admixture with the native T. cristatus -restricted at site BL173U, less than 2 km away from the introduction area-and no cytonuclear disequilibrium. Triturus newts commonly hybridize in natural (Arntzen, Wielstra, & Wallis, 2014;Maletzky et al, 2008) and invasive ranges, which contributes to the spread of T. carnifex genes (Brede, 2015;Dufresnes et al, 2016;Meilink, Arntzen, van Delft, & Wielstra, 2015;Wielstra et al, 2016). Widespread mtDNA introgression has been reported in Triturus invasions (Dufresnes et al, 2016), as also seen from their moving natural hybrid zones (e.g., Wielstra et al, 2017).…”

Section: Emerging Invasion Of Italian Crested Newtsmentioning

confidence: 99%

Early detection and spatial monitoring of an emerging biological invasion by population genetics and environmental DNA metabarcoding

Dufresnes

Déjean

Zumbach

et al. 2019

Conservat Sci and Prac

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Rapid management responses against invasive species soon after their establishment are the most efficient way to limit their biological and economic impacts. Early detection and reliable monitoring is however challenging when cryptic taxa are involved. Here we show how environmental DNA (eDNA) metabarcoding of water samples efficiently unveiled an emerging invasion of Italian crested newts (Triturus carnifex), one of the most successful amphibian invaders in Europe. We compared and validated an eDNA survey by multilocus population genetics of wild-caught individuals. Both approaches consistently mapped a localized T. carnifex invasion in northwestern Switzerland, most likely following imports from the Italian Po Plain. We found evidence of gene flow with the indigenous and endangered Triturus cristatus in nearby populations, suggesting a potential expansion. Yet the currently small invasive range should be efficiently contained by future eradication programs. This textbook case emphasizes the implementation of eDNA metabarcoding to screen aquatic communities for exotic species, from which targeted studies can be designed on emerging biological invasions.

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“…We used Kompetitive Allele Specific PCR (KASP) all-inclusive services to extract DNA and genotype 96 SNP loci in all 63 adults and 207 hatchlings (Semagn et al 2014). KASP has been successful for genotyping many crop species (McCouch et al 2010; Khera et al 2013), and its use in vertebrates is increasingly common (e.g., Wielstra et al 2016; Bourgeois et al 2018). To determine the 96 SNP loci that confidently assign parentage, we used RADseq data of known parent-offspring pairs from this population (FJ Janzen, unpublished data).…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and environmental predictors of reproductive success in painted turtles

Hoekstra

Holden

et al. 2020

Preprint

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Sexual selection is often assumed to elicit sexually dimorphic traits. However, most work on this assumption in tetrapod vertebrates has focused on birds. In this field experiment, we assessed relationships between both sexually dimorphic (body size, claw length) and non-dimorphic traits (forelimb stripe color, baseline corticosterone concentrations) and reproductive success in adult painted turtles to explicate the roles of these phenotypes in mate choice and the evolution of sexual dimorphism. We also modified adult sex ratios in experimental ponds to elucidate the role of biased sex ratios on reproductive success, which is a timely test of the potential threat of biased sex ratios on population persistence in a species with temperature-dependent sex determination. We found no strong influence of male phenotypes on male siring success, but female body size and baseline corticosterone concentrations predicted female clutch sizes. We find weak evidence that adult sex ratio influences male siring success, with a male-biased sex ratio producing lower male siring success than a female-biased sex ratio. This study offers evidence that female mate choice may not be an important selective force on male phenotypes, but that instead selection occurs on female phenotypes, particularly body size and corticosterone concentrations. Further, biased adult sex ratios can influence reproductive success of both sexes. Finally, the use of Kompetitive Allele Specific PCR (KASP) was highly successful in parentage analysis, which adds reptiles to the growing list of taxa successfully genotyped with this new technology.Lay SummaryFemale painted turtles aren’t choosy about traits of their mates. In a field experiment, we find that male traits do not predict male fitness, but key female traits (body size and stress levels) do predict female reproductive success. Further, we find weak evidence that adult sex ratio influences individual fitness in this species with environmental sex determination. Ultimately, we reject the long-assumed importance of female mate choice in this freshwater turtle.

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Efficient screening for ‘genetic pollution’ in an anthropogenic crested newt hybrid zone

Cited by 10 publications

References 30 publications

The genic view of hybridization in the Anthropocene

The genic view of hybridization in the Anthropocene

Early detection and spatial monitoring of an emerging biological invasion by population genetics and environmental DNA metabarcoding

Phenotypic and environmental predictors of reproductive success in painted turtles

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