Efficient saccharification for non-treated cassava pulp by supplementation of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase

Vaithanomsat, Pilanee; Kosugi, Akihiko; Apiwatanapiwat, Waraporn; Thanapase, Warunee; Waeonukul, Rattiya; Tachaapaikoon, Chakrit; Pason, Patthra; Mori, Yutaka

doi:10.1016/j.biortech.2012.11.023

Cited by 16 publications

(7 citation statements)

References 7 publications

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“…The β-glucosidase is capable of hydrolyzing cellobiose to form glucose utilized by C. thermocellum . In previous reports, remarkable improvements were demonstrated in saccharification for lignocellulosic materials by C. thermocellum in combination with a thermostable β-glucosidase. , In the present study, three main sugars (glucose, cellobiose, and xylose) could not be detected during the whole fermentation process, which was in accordance with our previous study and could be explained by the efficient uptake and utilization by C. thermocellum and T. aotearoense once the sugars were produced. The results imply that the promotion of the β-glucosidase activity may contribute to the enhancement of biodegradation of SCB by the coculture of C. thermocellum and T. aotearoense .…”

Section: Resultssupporting

confidence: 92%

Enhanced Biodegradation of Sugar Cane Bagasse by Coculture of Clostridium thermocellum and Thermoanaerobacterium aotearoense Supplemented with CaCO₃

Tian

Zhu

2017

Energy Fuels

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Pretreated sugar cane bagasse (SCB) could be directly degraded by a designed coculture system with Clostridium thermocellum and Thermoanaerobacterium aotearoense for biological hydrogen and ethanol production, and the production was remarkably improved by CaCO3 supplementation. Here, the effects of CaCO3 concentration (10–100 mM) on the production of hydrogen and ethanol were investigated. Under the optimal CaCO3 concentration of 40 mM, hydrogen production reached 87.56 ± 4.08 mmol/L from 2% pretreated SCB with a yield of 4.38 mmol H2/g SCB, an 88.62% increase over the culture without added CaCO3 (46.42 ± 1.22 mmol/L and 2.32 mmol H2/g SCB). Additionally, the maximum ethanol concentration reached 10.60 ± 0.81 mM, a 192.82% increase over the control (3.62 ± 0.14 mM). The stimulatory effect of CaCO3 on the biodegradation of SCB was primarily ascribed to the buffering capacity of CO3 2–.This study developed a novel strategy to improve SCB biodegradation for biofuel production.

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Section: Resultssupporting

confidence: 92%

Enhanced Biodegradation of Sugar Cane Bagasse by Coculture of Clostridium thermocellum and Thermoanaerobacterium aotearoense Supplemented with CaCO₃

Tian

Zhu

2017

Energy Fuels

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“…Vaithanomsat et al. have found that the supplementation of α‐amylase, cellulosome from C. thermocellum and β‐glucosidase from T. brockii achieved an efficient saccharification for cassava pulp. An efficient system (cultures/cellulosome of C. thermocellum mixed with thermostable β‐glucosidases) was developed by Prawitwong et al.…”

Section: The Application Of Cellulosome In Biorefinerymentioning

confidence: 99%

Reconstitution of cellulosome: Research progress and its application in biorefinery

Zhu

2019

Biotech and App Biochem

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Lignocellulose, one of the most abundant renewable sources of sugar, can be converted into bioenergy through hydrolysis of cellulose and hemicellulose. Due to its renewability and availability in large quantities, bioenergy is considered as a possible alternative to fossil energy and attracts the attention of the world with increased concerns about environmental protection and energy crisis. The depolymerization of cellulosic substrate to monomer is the rate‐limiting step in the bioconversion of lignocellulose by cellulolytic microbes. Cellulosome, a multienzyme complex from anaerobic cellulolytic bacteria, can efficiently degrade the cellulosic substrates. Previous studies have shown that the reconstitution of cellulosome in vitro and its heterologous expression or display on the cell surface can help to solve the low yield problem of cellulosome in cellulolytic bacteria. This paper reviews the research progress in the reconstitution of cellulosome as well as its application in biorefinery, including the construction of cellulosome as well as different methods for cellulosome reconstitution and its surface display. This review will promote the understanding of cellulosome and its reconstitution.

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“…There was a small amount of protein, ash, and fat. Cassava pulp generated in high quantities as a side-product of tapioca industry is a potential source of biomass (Vaithanomsat et al, 2016). The starch component of cassava pulp has a larger portion than the fiber component, while dietary fibers are components of non-starch carbohydrates.…”

Section: Chemical Composition Of Cassava Pulpmentioning

confidence: 99%

Process Optimization for Dietary Fiber Production from Cassava Pulp Using Acid Treatment

Pramana

Sunarti

Purwoko

2018

Acta Universitatis Cibiniensis. Series E: Food Technology

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Cassava pulp, the side product of tapioca industry consists of starch and fiber as the major component. Acid treatment was employed in the conversion process of cassava pulp into dietary fiber to remove the starch component, to increase fiber content, and to modify the structure of fiber. This study purposed to obtain optimum process conditions (acid concentration, temperature, and reaction time) in the production of dietary fiber from cassava pulp. Process optimization was conducted using Response Surface Methodology (RSM) for maximizing Total Dietary Fiber (TDF), Water Holding Capacity (WHC) and Oil Holding Capacity (OHC) as the responses. The optimum process was gained at 6% H2SO4 concentration, 127°C, and 45 mins. Prediction values of TDF, WHC, and OHC were 100%, 10.47 g/g, and 3.60 g/g, respectively. Validation was carried out and resulted in TDF 96.95%, WHC 10.47 g/g, and OHC 3.55 g/g. Physicochemical properties of the resulting dietary fiber were significantly improved. The fiber structure has modified which characterized by the changes in morphology and crystallinity.

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Efficient saccharification for non-treated cassava pulp by supplementation of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase

Cited by 16 publications

References 7 publications

Enhanced Biodegradation of Sugar Cane Bagasse by Coculture of Clostridium thermocellum and Thermoanaerobacterium aotearoense Supplemented with CaCO₃

Enhanced Biodegradation of Sugar Cane Bagasse by Coculture of Clostridium thermocellum and Thermoanaerobacterium aotearoense Supplemented with CaCO₃

Reconstitution of cellulosome: Research progress and its application in biorefinery

Process Optimization for Dietary Fiber Production from Cassava Pulp Using Acid Treatment

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Efficient saccharification for non-treated cassava pulp by supplementation of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase

Cited by 16 publications

References 7 publications

Enhanced Biodegradation of Sugar Cane Bagasse by Coculture of Clostridium thermocellum and Thermoanaerobacterium aotearoense Supplemented with CaCO3

Enhanced Biodegradation of Sugar Cane Bagasse by Coculture of Clostridium thermocellum and Thermoanaerobacterium aotearoense Supplemented with CaCO3

Reconstitution of cellulosome: Research progress and its application in biorefinery

Process Optimization for Dietary Fiber Production from Cassava Pulp Using Acid Treatment

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Product

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Enhanced Biodegradation of Sugar Cane Bagasse by Coculture of Clostridium thermocellum and Thermoanaerobacterium aotearoense Supplemented with CaCO₃

Enhanced Biodegradation of Sugar Cane Bagasse by Coculture of Clostridium thermocellum and Thermoanaerobacterium aotearoense Supplemented with CaCO₃