“…Enzymatic saccharification was done by a modification of the previous protocol of Park et al (Park et al 2009) with triplicate samples. To the SFR suspended in 500 µl of 50 mM sodium citrate (pH 4.8), 1 ml of enzyme solution composed of Celluclast 1.5 L (Novozymes, Bagsvaerd, Denmark) (1.1 FPU), Novozyme 188 (Novozymes, Bagsvaerd, Denmark) (2.5 CbU) and Ultra o L (Novozymes, Bagsvaerd, Denmark) (65 µg) in the same bu er.…”