Efficient purification of high‐purity compounds from the stem of Lonicera japonica Thunb using two‐dimensional preparative chromatography

Abstract

Purification of high-purity compounds from traditional Chinese medicines (TCMs) plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of TCMs in chemical composition. In this work, a two-dimensional preparation method was successfully developed for the preparation of high-purity compounds from the stem of Lonicera japonica Thunb, based on two novel polar copolymerized RP stationary phases, XAqua C3 and XAqua C18. An XAqua C3 prep column was used to separate the sample in the first-dimensional preparation, and 14 g of sample was fractionated into eight fractions with a recovery of 82%. An XAqua C18 prep column was selected to prepare high-purity compounds in the second-dimensional preparation for its good orthogonality with the XAqua C3 stationary phase. As a result, major compounds in the sample were isolated with more than 99% purity. This method is a potent method to realize the efficient purification of compounds with high purity from the stem of L. japonica Thunb and it shows great potential in the separation of high-purity compounds from complex samples.

Synthesis and evaluation of a maltose‐bonded silica gel stationary phase for hydrophilic interaction chromatography and its application in Ginkgo Biloba extract separation in two‐dimensional systems

“…More validation in practical analysis needed to be investigated. Thus, multidimensional liquid chromatograph technology arises at the historic moment for the analysis of real samples . In our study, it should be noted that the elution order of the compounds on maltose‐bonded stationary phase is very different from that on the RP‐HPLC stationary phase, which indicates that there is orthogonality between HILIC and RP‐HPLC chromatographic retention behavior .…”

Bioassay-Guided Separation and Identification of a New Anti-Lung Cancer Compound from Cordyceps militaris by Means of Off-Line Two-Dimensional Preparative Chromatography, Real-Time Cell Analysis, and X-ray Single-Crystal Diffraction

“…Fourteen fractions were collected and dried by rotary evaporation at 50 °C. Figure 1b shows four major and eight minor peaks eluting at 6,7,8,10,15,17,18,19,20,22,27,34,36, and 40 min; the amounts by weight ranged from approximately 0.08 g for fraction 9 to 4.89 g for fraction 10. Recovery from the bare silica DAC preparative column was approximately 68 %.…”

“…In previous research on 2D HPLC separation methods for botanical samples, the polar and weakly polar fractions were all removed by SPE before the samples were loaded on to the chromatographic column [34]. SPE columns could protect the chromatographic column from dye adsorption.…”

Preparation of two flavonoid glycosides with unique structures from barley seedlings by membrane separation technology and preparative high‐performance liquid chromatography

“…Preparative HPLC is a robust, versatile, and fairly rapid technique that provides advantages of high efficiency, high resolution, and good repeatability that are not found in other chromatographic techniques . It takes advantage of high‐performance separation, online detection, and automatic control to realize the efficient separation of target compounds .…”