Bioassay-Guided Separation and Identification of a New Anti-Lung Cancer Compound from Cordyceps militaris by Means of Off-Line Two-Dimensional Preparative Chromatography, Real-Time Cell Analysis, and X-ray Single-Crystal Diffraction

Abstract

has much potential for purification of other weakly polar compounds and could enable purification of other traditional Chinese medicines.

Counter-Current Fractionation-Assisted Bioassay-Guided Separation of Active Compound from Blueberry and the Interaction between the Active Compound and α-Glucosidase

“…By combining chromatography with biological activity detection, the active compounds were enriched to obtain high activity pure compounds [ 3 , 4 ]. Bioactive compounds, including antimicrobial coumarins, anti-lung cancer compound, and water-soluble antioxidants, have been successfully isolated by BGS [ 5 , 6 , 7 ]. Counter-current chromatography, as the most vital chromatographic technique in BGS, is often used for the separation of various active compounds from natural plant resources [ 8 , 9 ].…”

Bioactivity‐guided separation of antifungal compounds by preparative high‐performance liquid chromatography

“…Therefore, bioactivity-guided separation has gradually attracted the attention of researchers and it can be considered to be a process including a series of steps to locate the active compounds before the separation. Successful examples can be seen in the discovery of compounds with κ-opioid receptor agonist activities in Corydalis yanhusuo W. T. Wang [11] and anti-lung cancer compounds from Cordyceps militaris [12]. With the aid of the active screening of the fractions separated by HPLC, some of the components with outstanding activity can be locked, and then be separated again by prep-HPLC to obtain the active compounds.…”

Cytotoxic compounds against cancer cells from Bombyx mori inoculated with Cordyceps militaris

“…In the studies about Cordyceps sp., a lot of bioactivities have been reported, such as immunomodulatory activity, antioxidant activity, anti-tumor activity, and cancer cell cytotoxicity. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] Cordyceps militaris is a valuable specie of the genus Cordyceps. It parasitizes lepidopteran pupa and forms fruiting bodies.…”

“…Although several biological and chemical studies about the mycelia and the fruiting bodies have been reported, there are few studies about its host, lepidopteran larvae infested with the fungus. 4,7,8,10,12,13,[15][16][17][18][19][20] In the screening experiments, we found the cytotoxicity of the crude extracts of Bombyx mori inoculated with C. militaris and started this study.…”

“…1 H NMR spectra (one-and two-dimensional) were recorded on a Jeol lambda-500 spectrometer (Jeol Ltd., Tokyo, Japan) at 500 MHz, while 13 C NMR spectra were recorded by the same instrument at 125 MHz. HRESIMS data were measured by a JMS-T100LC mass spectrometer (Jeol Ltd., Tokyo, Japan).…”