Efficient production of succinic acid from duckweed (Landoltia punctata) hydrolysate by Actinobacillus succinogenes GXAS137

Shen, Naikun; Zhang, Hongyan; Qin, Yan; Wang, Qingyan; Zhu, Jing; Li, Yang; Jiang, Mingguo; Huang, Ribo

doi:10.1016/j.biortech.2017.09.208

Cited by 47 publications

(22 citation statements)

References 43 publications

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“…For example, in the subtropical climate of Eastern China, L. punctata is the first duckweed species to colonize water reservoirs in the Spring and the last remaining in the Fall, thus exhibiting the longest vegetative growth period compared with other duckweeds. Based on these qualities, the species has attracted much attention as a promising, inexpensive, and sustainable source of valuable biomass for the production of biofuels, such as ethanol, butanol, biogas, and hydrogen ( Tao et al, 2017 ; Toyama et al, 2018 ; Miranda et al, 2020 ), and high-value biochemicals, such as succinic acid ( Shen et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Mosaic Arrangement of the 5S rDNA in the Aquatic Plant Landoltia punctata (Lemnaceae)

2021

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Duckweeds are a group of monocotyledonous aquatic plants in the Araceae superfamily, represented by 37 species divided into five genera. Duckweeds are the fastest growing flowering plants and are distributed around the globe; moreover, these plants have multiple applications, including biomass production, wastewater remediation, and making pharmaceutical proteins. Dotted duckweed (Landoltia punctata), the sole species in genus Landoltia, is one of the most resilient duckweed species. The ribosomal DNA (rDNA) encodes the RNA components of ribosomes and represents a significant part of plant genomes but has not been comprehensively studied in duckweeds. Here, we characterized the 5S rDNA genes in L. punctata by cloning and sequencing 25 PCR fragments containing the 5S rDNA repeats. No length variation was detected in the 5S rDNA gene sequence, whereas the nontranscribed spacer (NTS) varied from 151 to 524 bp. The NTS variants were grouped into two major classes, which differed both in nucleotide sequence and the type and arrangement of the spacer subrepeats. The dominant class I NTS, with a characteristic 12-bp TC-rich sequence present in 3–18 copies, was classified into four subclasses, whereas the minor class II NTS, with shorter, 9-bp nucleotide repeats, was represented by two identical sequences. In addition to these diverse subrepeats, class I and class II NTSs differed in their representation of cis-elements and the patterns of predicted G-quadruplex structures, which may influence the transcription of the 5S rDNA. Similar to related duckweed species in the genus Spirodela, L. punctata has a relatively low rDNA copy number, but in contrast to Spirodela and the majority of other plants, the arrangement of the 5S rDNA units demonstrated an unusual, heterogeneous pattern in L. punctata, as revealed by analyzing clones containing double 5S rDNA neighboring units. Our findings may further stimulate the research on the evolution of the plant rDNA and discussion of the molecular forces driving homogenization of rDNA repeats in concerted evolution.

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Section: Introductionmentioning

confidence: 99%

Mosaic Arrangement of the 5S rDNA in the Aquatic Plant Landoltia punctata (Lemnaceae)

2021

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“…Plant residues, which are another lignocellulose‐rich material, were limited in their use for succinic acid production due to the complex and expensive pretreatment processes. After optimizing the technique of enzyme pretreatment and fermentation configuration, a maximum succinic acid concentration of 75.5 g L −1 and yield of 0.42 g g −1 were obtained from duckweed hydrolysate by A. succinogenes GXAS137 in 2 L stirred bioreactors within 56 h . Recently, a new wild‐type strain, Basfia succiniciproducens BPP7, was found to have the ability to utilize Arundo donax as feedstock for succinic acid production.…”

Section: Optimization Of Fermentation Conditions To Improve Succinic mentioning

confidence: 99%

Bio‐based succinic acid: an overview of strain development, substrate utilization, and downstream purification

Dai

Guo

Zhang

et al. 2019

Biofuels Bioprod Bioref

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Succinic acid is an industrially important platform chemical that could be used as precursor for the establishment of a sustainable chemical industry. Due to the depletion of crude oil and the need for sustainable development, the biological production of succinic acid from renewable resources has attracted wide attention. To establish an economical bio‐based succinic acid production process, various metabolic engineering strategies have been developed and applied to improve the strain performance, including efficient CO2 fixation, by‐product elimination, use of metabolic modeling approaches, engineering of transcriptional regulations and optimization of reducing power balance. The utilization of renewable waste resources and the optimization of purification process have also been investigated to decrease the cost. This review will provide insights for current advances in succinic acid production and perspectives on further research to make bio‐based succinic acid production more efficient and economical. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Succinic acid (SA) is an important C4-building chemical platform for many applications, including food, agriculture, and pharmaceutical ( 1 , 2 ) production. Commercial SA is mainly produced through petrochemical processes, which bring environmental pollution and other concerns related to sustainable development ( 3 , 4 ). As an alternative, SA can be manufactured using bio-based feedstock through microbial fermentation ( 5 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Actinobacillus succinogenes GXAS137, a Highly Efficient Producer of Succinic Acid

et al. 2018

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Efficient production of succinic acid from duckweed (Landoltia punctata) hydrolysate by Actinobacillus succinogenes GXAS137

Cited by 47 publications

References 43 publications

Mosaic Arrangement of the 5S rDNA in the Aquatic Plant Landoltia punctata (Lemnaceae)

Mosaic Arrangement of the 5S rDNA in the Aquatic Plant Landoltia punctata (Lemnaceae)

Bio‐based succinic acid: an overview of strain development, substrate utilization, and downstream purification

Complete Genome Sequence of Actinobacillus succinogenes GXAS137, a Highly Efficient Producer of Succinic Acid

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