Complete Genome Sequence of
            <i>Actinobacillus succinogenes</i>
            GXAS137, a Highly Efficient Producer of Succinic Acid

Zhang, Hongyan; Shen, Naikun; Qin, Yan; Zhu, Jing; Li, Yang; Wu, Jiafa; Jiang, Mingguo

doi:10.1128/genomea.01562-17

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…As far as A. succinogenes is concerned, a patent for the production of SA using this strain was registered in 1996 [19], and the first strain isolated from bovine rumen was published in 1999 [20]. [15,16], 2 [21], 3 [22], 4 [23], 5 [24], 6 [19], 7 [25], 8 [20], 9 [2], 10 [26], 11 [27], 12 [27], 13 [8], 14 [18], 15 [3], 16 [28], 17 [29], 18 [30].…”

Section: Major Randd Advancements On Sa and A Succinogenesmentioning

confidence: 99%

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Insights on the Advancements of In Silico Metabolic Studies of Succinic Acid Producing Microorganisms: A Review with Emphasis on Actinobacillus succinogenes

et al. 2021

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Succinic acid (SA) is one of the top candidate value-added chemicals that can be produced from biomass via microbial fermentation. A considerable number of cell factories have been proposed in the past two decades as native as well as non-native SA producers. Actinobacillus succinogenes is among the best and earliest known natural SA producers. However, its industrial application has not yet been realized due to various underlying challenges. Previous studies revealed that the optimization of environmental conditions alone could not entirely resolve these critical problems. On the other hand, microbial in silico metabolic modeling approaches have lately been the center of attention and have been applied for the efficient production of valuable commodities including SA. Then again, literature survey results indicated the absence of up-to-date reviews assessing this issue, specifically concerning SA production. Hence, this review was designed to discuss accomplishments and future perspectives of in silico studies on the metabolic capabilities of SA producers. Herein, research progress on SA and A. succinogenes, pathways involved in SA production, metabolic models of SA-producing microorganisms, and status, limitations and prospects on in silico studies of A. succinogenes were elaborated. All in all, this review is believed to provide insights to understand the current scenario and to develop efficient mathematical models for designing robust SA-producing microbial strains.

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Section: Major Randd Advancements On Sa and A Succinogenesmentioning

confidence: 99%

“…The shaded boxes represent A. succinogenes. The numbers represent the sources of the studies: 1 [15,16], 2 [21], 3[22], 4[23], 5[24], 6[19], 7[25], 8[20], 9[2],10 [26], 11[27], 12[27], 13[8], 14[18], 15[3], 16[28], 17[29], 18[30].…”

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confidence: 99%

Insights on the Advancements of In Silico Metabolic Studies of Succinic Acid Producing Microorganisms: A Review with Emphasis on Actinobacillus succinogenes

et al. 2021

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“…The results showed that the acid resistance mechanism of K. europaeus CGMCC 20445 was related to the expression of genes related to pentose phosphate, butyrate metabolism, tricarboxylic acid cycle, oxidative phosphorylation, and fatty acid biosynthesis pathways ( Wang et al 2021 ). The whole-genome sequencing of A. succinogenes GXAS137 has been obtained by Illumina technology and uploaded to Genbank under the accession number NHRD00000000.1 ( Zhang et al 2018 ). The present study explored the complex mechanisms by which NaHSO 3 reduces AC formation based on gene expression changes revealed using the Illumina RNA-seq technology.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Molecular Mechanisms of Acetic Acid Reduction by Adding NaHSO₃ in Actinobacillus succinogenes GXAS137

Li,

Song,

Zhang

et al. 2023

Polish Journal of Microbiology

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Acetic acid (AC) is a major by-product from fermentation processes for producing succinic acid (SA) using Actinobacillus succinogenes. Previous experiments have demonstrated that sodium bisulfate (NaHSO3) can significantly decrease AC production by A. succinogenes GXAS137 during SA fermentation. However, the mechanism of AC reduction is poorly understood. In this study, the transcriptional profiles of the strain were compared through Illumina RNA-seq to identify differentially expressed genes (DEGs). A total of 210 DEGs were identified by expression analysis: 83 and 127 genes up-regulated and down-regulated, respectively, in response to NaHSO3 treatment. The functional annotation analysis of DEGs showed that the genes were mainly involved in carbohydrates, inorganic ions, amino acid transport, metabolism, and energy production and conversion. The mechanisms of AC reduction might be related to two aspects: (i) the lipoic acid synthesis pathway (LipA, LipB) was significantly down-regulated, which blocked the pathway catalyzed by pyruvate dehydrogenase complex to synthesize acetyl-coenzyme A (acetyl-CoA) from pyruvate; (ii) the expression level of the gene encoding bifunctional acetaldehyde-alcohol dehydrogenase was significantly up-regulated, and this effect facilitated the synthesis of ethanol from acetyl-CoA. However, the reaction of NaHSO3 with the intermediate metabolite acetaldehyde blocked the production of ethanol and consumed acetyl-CoA, thereby decreasing AC production. Thus, our study provides new insights into the molecular mechanism of AC decreased underlying the treatment of NaHSO3 and will deepen the understanding of the complex regulatory mechanisms of A. succinogenes.

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“…It was reported that deletion of pflA can increase succinic acid production from glycerol in E. coli under anaerobic conditions (Mienda et al, 2018). Protein homology analysis method showed that pflA can activate pflB under anaerobic conditions in A. succinogenes (Zhang et al, 2018a). These data provide a novel gene knockout target for increasing succinate production in A. succinogenes .…”

Section: Introductionmentioning

confidence: 99%

Metabolic Regulation of Organic Acid Biosynthesis in Actinobacillus succinogenes

Zhang

Qiao

et al. 2019

Front. Bioeng. Biotechnol.

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Actinobacillus succinogenes is one of the most promising strains for succinic acid production; however, the lack of efficient genetic tools for strain modification development hinders its further application. In this study, a markerless knockout method for A. succinogenes using in-frame deletion was first developed. The resulting ΔpflA (encode pyruvate formate lyase 1-activating protein) strain displayed distinctive organic acid synthesis capacity under different cultivation modes. Additional acetate accumulation was observed in the ΔpflA strain relative to that of the wild type under aerobic conditions, indicating that acetate biosynthetic pathway was activated. Importantly, pyruvate was completely converted to lactate under anaerobic fermentation. The transcription analysis and enzyme assay revealed that the expression level and specific activity of lactate dehydrogenase (LDH) were significantly increased. In addition, the mRNA expression level of ldh was nearly increased 85-fold compared to that of the wild-type strain during aerobic–anaerobic dual-phase fermentation, resulting in 43.05 g/L lactate. These results demonstrate that pflA plays an important role in the regulation of C3 flux distribution. The deletion of pflA leads to the improvement of acetic acid production under aerobic conditions and activates lactic acid biosynthesis under anaerobic conditions. This study will help elaborate the mechanism governing organic acid biosynthesis in A. succinogenes.

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Complete Genome Sequence of Actinobacillus succinogenes GXAS137, a Highly Efficient Producer of Succinic Acid

Cited by 6 publications

References 18 publications

Insights on the Advancements of In Silico Metabolic Studies of Succinic Acid Producing Microorganisms: A Review with Emphasis on Actinobacillus succinogenes

Insights on the Advancements of In Silico Metabolic Studies of Succinic Acid Producing Microorganisms: A Review with Emphasis on Actinobacillus succinogenes

Comparative Transcriptome Analysis Reveals the Molecular Mechanisms of Acetic Acid Reduction by Adding NaHSO₃ in Actinobacillus succinogenes GXAS137

Metabolic Regulation of Organic Acid Biosynthesis in Actinobacillus succinogenes

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Complete Genome Sequence of Actinobacillus succinogenes GXAS137, a Highly Efficient Producer of Succinic Acid

Cited by 6 publications

References 18 publications

Insights on the Advancements of In Silico Metabolic Studies of Succinic Acid Producing Microorganisms: A Review with Emphasis on Actinobacillus succinogenes

Insights on the Advancements of In Silico Metabolic Studies of Succinic Acid Producing Microorganisms: A Review with Emphasis on Actinobacillus succinogenes

Comparative Transcriptome Analysis Reveals the Molecular Mechanisms of Acetic Acid Reduction by Adding NaHSO3 in Actinobacillus succinogenes GXAS137

Metabolic Regulation of Organic Acid Biosynthesis in Actinobacillus succinogenes

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Comparative Transcriptome Analysis Reveals the Molecular Mechanisms of Acetic Acid Reduction by Adding NaHSO₃ in Actinobacillus succinogenes GXAS137