Efficient production of l-lactic acid from cassava powder by Lactobacillus rhamnosus

Relative Catalytic Efficiency of ldhL - and ldhD -Encoded Products Is Crucial for Optical Purity of Lactic Acid Produced by Lactobacillus Strains

“…The use of lactic acid in the synthesis of polylactic acid has grown over the years, and high optical purity is an inevitable prerequisite for lactic acid polymerization (1, 23). Strains of Lactobacillus, the largest genus of lactic acid bacteria (LAB), are the most frequently used lactic acid producers (2,4,10,19,22,24), but the optical purities of lactic acid produced by various Lactobacillus strains are markedly different (Table 1) (3,15).The enzymes responsible for L-and D-lactic acid production are NAD-dependent L-lactate dehydrogenases (L-nLDHs) and NAD-dependent D-lactate dehydrogenases (D-nLDHs), respectively, which fall into two different families and are encoded by ldhL and ldhD, respectively (7,20). Lactate racemase, which converts L-lactic acid into D-lactic acid, has only been reported in a few DL-type Lactobacillus strains.…”

“…The use of lactic acid in the synthesis of polylactic acid has grown over the years, and high optical purity is an inevitable prerequisite for lactic acid polymerization (1,23). Strains of Lactobacillus, the largest genus of lactic acid bacteria (LAB), are the most frequently used lactic acid producers (2,4,10,19,22,24), but the optical purities of lactic acid produced by various Lactobacillus strains are markedly different (Table 1) (3,15).…”

Screening de variáveis para a produção de biomassa e ácido lático utilizando planejamento Plackett-Burman

“…Já para a produção de ácido lático, o melhor experimento foi o 9, com produção de 32,26 g/L. Estudo realizado por Wang [21], foi encontrado 175,4 g/L de ácido lático na fermentação em batelada, quando trabalharam com pó de mandioca, onde neste pó havia uma concentração total de açúcar de 222,5 g/L.…”

Genome Sequence of Lactobacillus rhamnosus Strain CASL, an Efficient l -Lactic Acid Producer from Cheap Substrate Cassava

“…Therefore, the demand for L-lactic acid is continuously increasing (2,8). Because of their high levels of L-lactic acid production, some L. rhamnosus strains have been suggested to be good producers with great industrial potential (9).…”

“…This strain can also produce a high concentration of L-lactic acid (175 g/liter) from cassava powder in a simultaneous saccharification and fermentation process. This is the highest L-lactic acid concentration reported from a cassava source, and it provides an efficient L-lactic acid production process with this cheap raw bioresource (9). The strain has been deposited in the China General Microbiological Culture Collection Center (CGMCC no.…”