Genome Sequence of Lactobacillus rhamnosus Strain CASL, an Efficient
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            -Lactic Acid Producer from Cheap Substrate Cassava

Yu, Bo; Su, Fei; Wang, Limin; Zhao, Bo; Qin, Jiayang; Ma, Cuiqing; Xu, Ping; Ma, Yanhe

doi:10.1128/jb.06285-11

Cited by 7 publications

(4 citation statements)

References 10 publications

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“…Genomes of strains isolated from industrial fermentations have also been sequenced, including the cheese production isolate L. rhamnosus LC705 (15) and the beer spoilage isolate L. rhamnosus ATCC 8530 (16), and the genomes sequenced also include an environmental soil isolate, L. rhamnosus CASL (17).…”

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confidence: 99%

Correlation of Lactobacillus rhamnosus Genotypes and Carbohydrate Utilization Signatures Determined by Phenotype Profiling

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Lambert

Limpt

et al. 2015

Appl Environ Microbiol

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e Lactobacillus rhamnosus is a bacterial species commonly colonizing the gastrointestinal (GI) tract of humans and also frequently used in food products. While some strains have been studied extensively, physiological variability among isolates of the species found in healthy humans or their diet is largely unexplored. The aim of this study was to characterize the diversity of carbohydrate utilization capabilities of human isolates and food-derived strains of L. rhamnosus in relation to their niche of isolation and genotype. We investigated the genotypic and phenotypic diversity of 25 out of 65 L. rhamnosus strains from various niches, mainly human feces and fermented dairy products. Genetic fingerprinting of the strains by amplified fragment length polymorphism (AFLP) identified 11 distinct subgroups at 70% similarity and suggested niche enrichment within particular genetic clades. High-resolution carbohydrate utilization profiling (OmniLog) identified 14 carbon sources that could be used by all of the strains tested for growth, while the utilization of 58 carbon sources differed significantly between strains, enabling the stratification of L. rhamnosus strains into three metabolic clusters that partially correlate with the genotypic clades but appear uncorrelated with the strain's origin of isolation. Draft genome sequences of 8 strains were generated and employed in a gene-trait matching (GTM) analysis together with the publicly available genomes of L. rhamnosus GG (ATCC 53103) and HN001 for several carbohydrates that were distinct for the different metabolic clusters: L-rhamnose, cellobiose, L-sorbose, and ␣-methyl-Dglucoside. From the analysis, candidate genes were identified that correlate with L-sorbose and ␣-methyl-D-glucoside utilization, and the proposed function of these genes could be confirmed by heterologous expression in a strain lacking the genes. This study expands our insight into the phenotypic and genotypic diversity of the species L. rhamnosus and explores the relationships between specific carbohydrate utilization capacities and genotype and/or niche adaptation of this species.

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confidence: 99%

Correlation of Lactobacillus rhamnosus Genotypes and Carbohydrate Utilization Signatures Determined by Phenotype Profiling

Ceapă

Lambert

Limpt

et al. 2015

Appl Environ Microbiol

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“…The use of lactic acid in the synthesis of polylactic acid has grown over the years, and high optical purity is an inevitable prerequisite for lactic acid polymerization (1, 23). Strains of Lactobacillus, the largest genus of lactic acid bacteria (LAB), are the most frequently used lactic acid producers (2,4,10,19,22,24), but the optical purities of lactic acid produced by various Lactobacillus strains are markedly different (Table 1) (3,15).The enzymes responsible for L-and D-lactic acid production are NAD-dependent L-lactate dehydrogenases (L-nLDHs) and NAD-dependent D-lactate dehydrogenases (D-nLDHs), respectively, which fall into two different families and are encoded by ldhL and ldhD, respectively (7,20). Lactate racemase, which converts L-lactic acid into D-lactic acid, has only been reported in a few DL-type Lactobacillus strains.…”

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confidence: 99%

Relative Catalytic Efficiency of ldhL - and ldhD -Encoded Products Is Crucial for Optical Purity of Lactic Acid Produced by Lactobacillus Strains

Zheng

Sheng

et al. 2012

Appl Environ Microbiol

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“…(Prisciandaro et al, 2011) and soil derived L. rhamnosus CASL (Yu et al, 2011). In the current study, 70 strains of L. rhamnosus were successfully isolated from different Chinese subjects from different regions, and their draft-genomes were sequenced to determine the genetic diversity and phylogenetic correlations within the species.…”

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confidence: 99%

Comparative genomic analyses of Lactobacillus rhamnosus isolated from Chinese subjects

et al. 2020

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Genome Sequence of Lactobacillus rhamnosus Strain CASL, an Efficient l -Lactic Acid Producer from Cheap Substrate Cassava

Cited by 7 publications

References 10 publications

Correlation of Lactobacillus rhamnosus Genotypes and Carbohydrate Utilization Signatures Determined by Phenotype Profiling

Correlation of Lactobacillus rhamnosus Genotypes and Carbohydrate Utilization Signatures Determined by Phenotype Profiling

Relative Catalytic Efficiency of ldhL - and ldhD -Encoded Products Is Crucial for Optical Purity of Lactic Acid Produced by Lactobacillus Strains

Comparative genomic analyses of Lactobacillus rhamnosus isolated from Chinese subjects

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