Efficient production and secretion of bovine β-lactoglobulin by Lactobacillus casei

Abstract

Background: Lactic acid bacteria (LAB) are attractive tools to deliver therapeutic molecules at the mucosal level. The model LAB Lactococcus lactis has been intensively used to produce and deliver such heterologous proteins. However, compared to recombinant lactococci, lactobacilli offer some advantages such as better survival in the digestive tract and immunomodulatory properties. Here, we compared different strategies to optimize the production of bovine β-lactoglobulin (BLG), a major cow's milk allergen, in the probiotic strain Lactobacillus casei BL23.

In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells

“…2,25,26 In all the bacteria tested, significant amounts of BLGn were never produced in contrast to BLGn in eukaryotic cells. 26 For example, a maximum of B1% of BLG was produced in its native conformation in E. coli and L. lactis.…”

“…26 For example, a maximum of B1% of BLG was produced in its native conformation in E. coli and L. lactis. 2,25 In this study, we used the strain LL(pIL:BLG1), which is not able to produce BLG 3 and BLG was only detected under its native conformation. This is an evidence of both BLG synthesis in cells of the murine intestinal membrane and of plasmid translocation from L. lactis to the murine intestinal membrane.…”

Secretion of Insulinotropic Proteins by Commensal Bacteria: Rewiring the Gut To Treat Diabetes

“…Potential advantages of using commensal strains for in vivo recombinant gene expression include their compatibility with the host (particularly the host's immune system), their controllable persistence in the gut, and their ability to be orally dosed. Commensal bacterial expression of various recombinant cytokines and antigens in animal models has been reported (4,6,8,9). We recently engineered Escherichia coli Nissle 1917 (an over-thecounter probiotic strain, hereinafter referred to as Nissle) for the expression of a Vibrio cholerae quorum-sensing signal, creating a potential prophylactic for cholera (5).…”

Streptococcus thermophilus, an emerging and promising tool for heterologous expression: Advantages and future trends

“…It has also been shown in Lactobacillus casei and Lc. lactis that it is possible to prevent or treat cows' milk-related allergies using strains expressing the bovine b-lactoglobulin (Hazebrouck et al, 2007;Cortes-Perez et al, 2009). Such molecules could be produced by a S. thermophilus strain which additionally can be resistant to technological stress and be used to manufacture dairy products.…”