Efficient plastid transformation in tobacco using small gold particles (0.07^|^#8211;0.3^|^#8201;^|^micro;m)

Okuzaki, Ayako; Kida, Shoko; Watanabe, Junpei; Hirasawa, Izumi; Tabei, Yutaka

doi:10.5511/plantbiotechnology.12.1227a

Cited by 8 publications

(6 citation statements)

References 44 publications

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“…The most common approach for experimental delivery of foreign DNA has been particle bombardment (Bock, 2015). Tungsten and, more commonly, gold particles have been used to carry DNA plasmids into plant cells where contact with the plastid membrane can facilitate DNA entry (Svab et al, 1990; Daniell, 1993; Langbecker et al, 2004; Okuzaki et al, 2013). Another approach involving incubation of protoplasts (plant cells subjected to enzymatic degradation of the cell wall) with polyethylene glycol (PEG) and vector DNA has also been reported to produce plastid transformants (Golds et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Plastome Sequencing of Ten Nonmodel Crop Species Uncovers a Large Insertion of Mitochondrial DNA in Cashew

Rabah¹,

Lee

Hajrah

et al. 2017

The Plant Genome

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In plant evolution, intracellular gene transfer (IGT) is a prevalent, ongoing process. While nuclear and mitochondrial genomes are known to integrate foreign DNA via IGT and horizontal gene transfer (HGT), plastid genomes (plastomes) have resisted foreign DNA incorporation and only recently has IGT been uncovered in the plastomes of a few land plants. In this study, we completed plastome sequences for l0 crop species and describe a number of structural features including variation in gene and intron content, inversions, and expansion and contraction of the inverted repeat (IR). We identified a putative rpl22 in cinnamon (Cinnamomum verum J. Presl) and other sequenced Lauraceae and an apparent functional transfer of rpl23 to the nucleus of quinoa (Chenopodium quinoa Willd.). In the orchard tree cashew (Anacardium occidentale L.), we report the insertion of an ~6.7-kb fragment of mitochondrial DNA into the plastome IR. BLASTn analyses returned high identity hits to mitogenome sequences including an intact ccmB open reading frame. Using three plastome markers for five species of Anacardium, we generated a phylogeny to investigate the distribution and timing of the insertion. Four species share the insertion, suggesting that this event occurred <20 million yr ago in a single clade in the genus. Our study extends the observation of mitochondrial to plastome IGT to include long-lived tree species. While previous studies have suggested possible mechanisms facilitating IGT to the plastome, more examples of this phenomenon, along with more complete mitogenome sequences, will be required before a common, or variable, mechanism can be elucidated. The emergence of contemporary genomics has dispelled long-held hypotheses fueled by the Darwinian notion of evolution by vertical decent with modification. Drawing on phenotypic data, early investigators could not have predicted the impact of HGT on both the universality of the genetic code and diversity of organisms Abbreviations: aa, amino acid; ARF, auxin response factor; GC, guanine-cytosine; GSAF, Genome Sequencing and Analysis Facility; HGT, horizontal gene transfer; IGT, intracellular gene transfer; IR, inverted repeat; LSC, large single copy; MAFFT, multiple alignment using fast Fourier transform; MCS, membrane contact sites; ML, maximum likelihood; mtDNA, mitochondrial DNA; ncDNA, nuclear DNA; PCR, polymerase chain reaction; PEG, polyethylene glycol; ptDNA, plastid DNA; SC, single copy; SSC, small single copy; TACC, Texas Advanced Computing Center; UT-Austin, University of Texas-Austin. Core Ideas• DNA sequence data provides valuable information for biotechnology and evolutionary studies.• Plastid genomes (plastomes) of 10 nonmodel crop species were sequenced.• Inversions, gene divergence and loss, and IR boundary variation were identified.• Transfer of mitochondrial DNA to the plastome was found in Anacardium (cashew).

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Section: Discussionmentioning

confidence: 99%

Plastome Sequencing of Ten Nonmodel Crop Species Uncovers a Large Insertion of Mitochondrial DNA in Cashew

Rabah¹,

Lee

Hajrah

et al. 2017

The Plant Genome

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“…These nanoparticles upon conjugation with carbon nanoparticles delivered DNA to tobacco cells highly efficiently. 152 The same mycogenic nanoparticles were used in monocot species such as O. sativa and resulted in enhanced DNA delivery with minimum damage to the cell. 145 The metallic nanoparticles need less plasmid to carry DNA with low toxicity to plant cells in comparison to those other commercially available nanoparticles.…”

Section: ■ Transport and Delivery Systemsmentioning

confidence: 99%

“…The mycogenic nanoparticles that are produced by the fungus Aspergillus ochraceus are very small (5–25 nm). These nanoparticles upon conjugation with carbon nanoparticles delivered DNA to tobacco cells highly efficiently . The same mycogenic nanoparticles were used in monocot species such as O. sativa and resulted in enhanced DNA delivery with minimum damage to the cell .…”

Section: Nanoplatforms For Dna Deliverymentioning

confidence: 99%

Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions

Mujtaba

Wang

Carvalho

et al. 2021

ACS Agric. Sci. Technol.

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Current trends in plant genetic transformation technologies, i.e., designing and applying molecules like miRNA, RNAi, and CRISPR-Cas, largely enable researchers to target specific sites in the plant genome to avert the growing biotic and abiotic threats to plants. However, the delivery of these molecules through conventional techniques brings an array of drawbacks such as low efficiency due to the cell wall barrier, tissue damage that leads to browning or necrosis, degradation of these biomolecules by physiological conditions (high temperature, harsh pH, and light), and plant-specific protocols. The advancements in nanotechnology offer an excellent alternative for the safe and highly efficient delivery of biomolecules such as miRNA, CRISPR-Cas, and RNAi without damaging the plant tissues. Nanoparticle (polymeric, metallic, magnetic, silica, carbon, etc.)-based delivery of biomolecules can be efficiently utilized especially for plant protection applications. Herein, we present a comprehensive overview of current trends (with a focus on the previous five years) in nanoparticle-based delivery of miRNA, RNAi, CRISPR-Cas and simillar biomolecules for plant protection applications. In addition, a future perspective focuses on the research gaps and unexplored potentials of nanoparticles for the delivery of biomolecules.

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“…Biolistic delivery, also referred to as particle bombardment, can be used to facilitate the transient introduction of exogenous substances into cells (Sanford 2000 ). This technique has been widely used to transfer genes into cells and even into organelles (Okuzaki et al 2013 ) in a range of plant species (Wang and Jiang 2011 ). Notably, in recent years, biolistic delivery has been applied to deliver the CRISPR/Cas9 cassette into immature maize embryos (Svitashev et al 2016 , 2015 ) and wheat embryos (Hamada et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Detection of a biolistic delivery of fluorescent markers and CRISPR/Cas9 to the pollen tube

2021

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Key message Biolistic delivery into pollen. Abstract In recent years, genome editing techniques, such as the CRISPR/Cas9 system, have been highlighted as a new approach to plant breeding. Agrobacterium-mediated transformation has been widely utilized to generate transgenic plants by introducing plasmid DNA containing CRISPR/Cas9 into plant cells. However, this method has general limitations, such as the limited host range of Agrobacterium and difficulties in tissue culture, including callus induction and regeneration. To avoid these issues, we developed a method to genetically modify germ cells without the need for Agrobacterium-mediated transfection and tissue culture using tobacco as a model. In this study, plasmid DNA containing sequences of Cas9, guide RNA, and fluorescent reporter was introduced into pollen using a biolistic delivery system. Based on the transient expression of fluorescent reporters, the Arabidopsis UBQ10 promoter was found to be the most suitable promoter for driving the expression of the delivered gene in pollen tubes. We also evaluated the delivery efficiency in male germ cells in the pollen by expression of the introduced fluorescent marker. Mutations were detected in the target gene in the genomic DNA extracted from CRISPR/Cas9-introduced pollen tubes, but were not detected in the negative control. Bombarded pollen germinated pollen tubes and delivered their contents into the ovules in vivo. Although it is necessary to improve biolistic delivery efficiency and establish a method for the screening of genome-modified seeds, our findings provide important insights for the detection and production of genome-modified seeds by pollen biolistic delivery.

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Efficient plastid transformation in tobacco using small gold particles (0.07^|^#8211;0.3^|^#8201;^|^micro;m)

Cited by 8 publications

References 44 publications

Plastome Sequencing of Ten Nonmodel Crop Species Uncovers a Large Insertion of Mitochondrial DNA in Cashew

Plastome Sequencing of Ten Nonmodel Crop Species Uncovers a Large Insertion of Mitochondrial DNA in Cashew

Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions

Detection of a biolistic delivery of fluorescent markers and CRISPR/Cas9 to the pollen tube

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