Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators

Dhar, Uppeandra; Joshi, Mitali Yeshwant

doi:10.1007/s00299-005-0932-1

Cited by 80 publications

(59 citation statements)

References 18 publications

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“…In the present study, leaf is a better explant source for the species with its 100% of callus induced in MS medium supplemented with BA and NAA. This result was fairly consistent with other species tested [28,33,37]. The intercalary meristems distributed in leaves might be responsible for the higher regeneration potential.…”

Section: Discussionsupporting

confidence: 81%

“…Previous reports showed that PGR requirements for callus induction vary depending on the explants types in Asteraceae species [33][34][35], and such variations can be attributed to the physiological condition of the explant, which is determined by genetic factors [36]. In the present study, leaf is a better explant source for the species with its 100% of callus induced in MS medium supplemented with BA and NAA.…”

Section: Discussionmentioning

confidence: 66%

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Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

Kang

Sheng

et al. 2012

Acta Soc Bot Pol

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Goldenrod (Solidago canadensis L.) is an invasive plant species in many countries except North America but a cut-flower species worldwide. There is a need to generate and propagate goldenrod clones efficiently for research and commercial purposes. A callus induction and plantlet regeneration system was developed by studying the influence of explant type and different concentrations of plant growth regulators. The highest callus production from leaf segments was obtained on Murashige and Skoog’s medium (MS medium) supplemented with 1.0 mg/L naphthalene acetic acid (NAA) and 1.0 mg/L 6-benzylaminopurine (BA). Adventitious shoots could be regenerated directly from leaf explants without an intermediate callus phase with the highest shoot induction percentage of 87.2%. The largest number of adventitious shoots per leaf explant (3.2) was obtained on MS medium supplemented with 0.4 mg/L NAA and 2.0 mg/L BA. MS medium supplemented with 0.1 mg/L NAA and 1.0 mg/L BA was the best medium for axillary shoot regeneration from nodal segments. The highest root number and longest roots occurred on half-strength MS without the addition of any growth regulator. Rooted plantlets were then transferred to a soil-based growth medium, placed in a greenhouse, and acclimatized with 100% success. All surviving plants grew normally without showing any morphological variation when compared to those grow from seed. This regeneration protocol may be used to produce certain biotypes of goldenrod suitable for genetic transformation rapid propagation of goldenrod for commercial purposes or for screening fungi and toxins as potential biocontrol agents against this weed

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 66%

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

Kang

Sheng

et al. 2012

Acta Soc Bot Pol

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“…Acacia sinuata (Vengadesan et al 2002) and Tamarindus indica (Muhammad and Amusa 2003). The explant dependent callus proliferation and inter-explant variability is a common phenomenon usually observed during the callus induction in some systems (Arockiasamy and Ignacimuthu 1998;Pereira et al 2000;Dhar and Joshi 2005;Thomas and Maseena 2006). Regardless of cytokinin type and concentration, the amount of callus produced increased significantly when 0.5 mgl −1 NAA was incorporated in the medium.…”

Section: Discussionmentioning

confidence: 99%

An efficient plant regeneration system through callus for Pseudarthria viscida (L.) Wright and Arn., a rare ethnomedicinal herb

Cheruvathur

Thomas

2011

Physiol Mol Biol Plants

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The purpose of this study was to develop a protocol to induce high frequency of callus and subsequent plantlet regeneration for Pseudarthria viscida; an important medicinal plant. The cotyledonary node and young leaf pieces (1× 0.5 cm, length × breadth) were used as explants for callus induction and subsequent shoot regeneration and adventitious roots induction from the shoots. The best results were achieved on the following media: (1) 96 % callus induction from cotyledonary node explants on MS medium supplemented with 1.5 mgl −1 2, 4 dichlorophenoxyacetic acid ) after 45 days. The plantlets were transferred to the field after acclimatization. Of the 40 plantlets transplanted to the soil, 29 survived (72.5 %).

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“…Irrespective of the culture media and cultivar, leaf tissues gave the best yield of callusing. Leaves are known to be most totipotent, capable of becoming de-differentiated tissues (Dhar and Joshi, 2005). In Zinnia cell cultures, a perfect model for cell wall synthesis and programmed cell death in plants, mesophyll cells of young leaves are known to be highly totipotent.…”

Section: Discussionmentioning

confidence: 99%

Effect of 2,4-D, explants type and cultivar on the callogenesis expression of cassava (Manihot esculenta Crantz) in Ghana

Fletcher¹,

Amoako²,

Twumasi³

2011

Afr. J. Biotechnol.

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Modern biotechnological applications in breeding programmes for economically-important crops like cassava begins with successful establishment of stable and active callus cultures for somatic embryos which can be genetically manipulated and induced into planting materials. We have investigated the effects of explants (leaves, stem petiole and axillary buds), auxin (2, 4-D) concentrations (8, 12 and 15 mg/l) and cultivar in the induction of callus cultures from local cassava varieties in Ghana -Tuaka, Afisiafi, Afebankye and Doku. Callus formation was successfully induced in all explants (leaf, petiole and auxiliary buds) from the four cassava cultivars. Generally, there were no significant differences in callogenesis of the different explants at the three different 2,4-D concentrations (p < 0.05) producing 75% callus for explants. The stem petiole or stem bark produced the least percentage of callus per cultivar at all the auxin concentrations. However, the leaf explants were superior in callus formation in all the cassava varieties. Although, statistically insignificant, the 8 mg/l 2,4-D concentration was visually the best in callus formation from explants both within and across the cassava cultivars. This study had shown that different cassava explants respond differently to tissue culture conditions established for callus culture formation.

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Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew. (Asteraceae): effect of explant type, age and plant growth regulators

Cited by 80 publications

References 18 publications

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

Propagation of goldenrod (Solidago canadensis L.) from leaf and nodal explants

An efficient plant regeneration system through callus for Pseudarthria viscida (L.) Wright and Arn., a rare ethnomedicinal herb

Effect of 2,4-D, explants type and cultivar on the callogenesis expression of cassava (Manihot esculenta Crantz) in Ghana

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