An efficient plant regeneration system through callus for Pseudarthria viscida (L.) Wright and Arn., a rare ethnomedicinal herb

Cheruvathur, Meena K.; Thomas, T. Dennis

doi:10.1007/s12298-011-0089-z

Cited by 9 publications

(5 citation statements)

References 23 publications

(19 reference statements)

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“…Sulaiman and Toma [ 25 ] achieved successful callus induction in Arbutus andrachne using nodal explants. Similar results have also been documented in other plants, including Pseudarthria viscida [ 26 ] and Lycopersicon esculentum [ 27 ]. Plant nodes contain meristematic tissues that are easy to reactivate, with high concentrations of plant hormones (cytokinins and auxins) in the plant body providing high callus productivity [ 28 ].…”

Section: Discussionsupporting

confidence: 88%

In vitro callus induction and evaluation of antioxidant activity of Rhinacanthus nasutus (L.) Kurz

Yaowachai,

Luecha,

Taratima

2023

Biology Methods and Protocols

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Rhinacanthus nasutus (L.) Kurz is used in Thai traditional medicine for the treatment of skin diseases, ringworm, and eczema. This research studied the effects of cytokinin and auxins on callus induction and evaluated antioxidant activity of R. nasutus. Nodes, young, and mature leaf explants were cultured on MS medium containing 0, 1, 2, 3, and 4 mg/L Kinetin (6-furfurylaminopurine) and 0, 1 mg/L NAA (1-naphthaleneacetic acid), IAA (indole-3-acetic acid), and 2,4-D (2,4-dichlorophenoxyacetic acid) for 6 weeks to induce callus. Calli derived from nodes, young and mature leaves, and other plant parts were ultrasonically extracted with methanol to determine total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity by FRAP, DPPH and ABTS assays. Nodal explants on MS medium containing 1 mg/L Kinetin combined with 1 mg/L 2,4-D were most efficient in callus production with the average fresh and dry weight per explant of 2.29 ± 0.14 g and 0.18 ± 0.01 g, respectively. Addition of Kinetin combined with NAA or 2,4-D had a positive effect on callus induction from young and mature leaf explants. The leaf extract showed the highest TPC, TFC, FRAP and IC50 of DPPH and ABTS assays (ca 113 mg GAE/g extract, 45 mg QE/g extract, 121 mg TE/g extract, 53 μg/mL and 14 μg/mL, respectively), followed by callus derived from nodes. Overall, phenolic content was higher than flavonoid content. A strong positive correlation was found between FRAP assay, TPC (r = 0.973) and TFC (r = 0.798), indicating that phenolic and flavonoid compounds are responsible for antioxidant activity of R. nasutus.

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Section: Discussionsupporting

confidence: 88%

In vitro callus induction and evaluation of antioxidant activity of Rhinacanthus nasutus (L.) Kurz

Yaowachai,

Luecha,

Taratima

2023

Biology Methods and Protocols

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“…In Dorema ammoniacum, various explants such as root, hypocotyls and cotyledon segments were used for callus induction and the best response was observed from root segments (Irvani et al 2010). Similarly, in Pseudarthria viscid also callus formation was affected by the source of the explants and cotyledonary node was most responsive for callus induction followed by leaf explants (Cheruvathur and Thomas 2011). In this study, 2, 4-D was found to be essential for callus induction as well as multiplication.…”

Section: Discussionmentioning

confidence: 59%

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

Abraham

Thomas

2015

Physiol Mol Biol Plants

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An efficient callus induction and plant regeneration system has been standardized for an ethnomedicinal plant, Elephantopus scaber Linn. Two explants i. e. seeds and leaf segments were used for callus induction. Murashige and Skoog (MS) medium supplemented with 5.0 μM 2, 4-dichlorophenoxy acetic acid (2, 4-D) and 0.5 μM kinetin (Kn) gave the optimum frequency (89 %) of callus induction from seed explant. The results showed that the highest response in terms of percent callus regenerating (91 %) and number of shoots (56) per culture was recorded on MS medium supplemented with 6.0 μM N6-benzylaminopurine (BA) and 1.5 μM α naphthalene acetic acid (NAA). The best rooting of regenerated shoots was obtained on half strength MS medium supplemented with 6.0 μM indole-3- butyric acid (IBA). On this medium, 100 % of the shoots produced roots with a mean number of 3.2 roots per shoot. The positive role of vesicular arbuscular mycorrhizae (VAM) along with potting mix has been well established in the present study. Of the various potting mix employed for plant acclimatization, the highest response of 100 % plant survival was noticed when autoclaved garden soil, sand (2:1) and VAM was utilized as potting mix. Inter-simple sequence repeats (ISSR) were used to establish the clonal fidelity of regenerated plantlets and the banding profiles from callus derived plants were monomorphic and similar to those of mother plant, thus ascertaining the true-to-type nature of these plants.

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“…In vitro propagation has been successfully applied for the conservation of a large number of endangered plant species that have limited reproductive capacity and exist in threatened habitats (Fay 1992;George et al 2008;Pence 2010). Recently, there have been a number of established protocols for in vitro propagation of rare and threatened medicinal plants such as Dysosma versipellis (Jiang et al 2012), Polygonatum verticillatum (Bisht et al 2012), Pseudarthria viscida (Cheruvathur and Thomas 2011), and Thymus lotocephalus (Coelho et al 2012).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant

Prathanturarug

Pheakkoet

Jenjittikul

et al. 2012

Physiol Mol Biol Plants

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In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered species of Stemonaceae, was established. Nodal and internodal explants were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) alone or in combination with 1-naphthaleneacetic acid (NAA). When cultured on MS medium supplemented with 18.16 μM TDZ with 0.54 μM NAA for 8 weeks and then on MS medium without plant growth regulator (PGR) for 8 weeks, nodal explants demonstrated a responding frequency of 91.67 % and a shoot regeneration rate of 5.46 shoots/responding explant. Furthermore, internodal explants demonstrated a responding frequency of 17.17 % with 11.17 shoots/responding explant. The regenerated shoots were rooted using a two-step protocol by culturing for 4 weeks on MS medium supplemented with various concentrations of NAA and Indole-3-butyric acid (IBA) and 8 weeks on MS medium without PGR. When 5.40 μM NAA was used as a PGR, the maximum root induction rate was 69.45 %. Regenerated plantlets were successfully transferred to soil.

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An efficient plant regeneration system through callus for Pseudarthria viscida (L.) Wright and Arn., a rare ethnomedicinal herb

Cited by 9 publications

References 23 publications

In vitro callus induction and evaluation of antioxidant activity of Rhinacanthus nasutus (L.) Kurz

In vitro callus induction and evaluation of antioxidant activity of Rhinacanthus nasutus (L.) Kurz

Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb

In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant

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