Efficient myogenic commitment of human mesenchymal stem cells on biomimetic materials replicating myoblast topography

Lee, Eunjee A.; Im, Seyoung; Hwang, Nathaniel S.

doi:10.1002/biot.201400020

Cited by 23 publications

(22 citation statements)

References 47 publications

(42 reference statements)

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“…Topographical cues have been shown to enhance myogenic differentiation and maturation of myoblasts [18] and to induce myogenic commitment of human mesenchymal stem cells in vitro [19]. Surface topography alone [20] or in combination with substrate rigidity [21] have been shown to control mesenchymal stem cell lineage commitment.…”

Section: Introductionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractControlling the cell-substrate interactions at the bio-interface is becoming an inherent element in the design of implantable devices. Modulation of cellular adhesion in vitro, through topographical cues, is a well-documented process that offers control over subsequent cellular functions. However, it is still unclear whether surface topography can be translated into a clinically functional response in vivo at the tissue / device interface. Herein, we demonstrated that anisotropic substrates with a groove depth of ~317 nm and ~1,988 nm promoted human tenocyte alignment parallel to the underlying topography in vitro. However, the rigid poly(lactic-co-glycolic acid) substrates used in this study upregulated the expression of chondrogenic and osteogenic genes, indicating possible tenocyte trans-differentiation. Of significant importance is that none of the topographies assessed (~37 nm, ~317 nm and ~1,988 nm groove depth) induced extracellular matrix orientation parallel to the substrate orientation in a rat patellar tendon model. These data indicate that two-dimensional imprinting technologies are useful tools for in vitro cell phenotype maintenance, rather than for organised neotissue formation in vivo, should multifactorial approaches that consider both surface topography and substrate rigidity be established.

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Section: Introductionmentioning

confidence: 99%

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

et al. 2015

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“…A) . Indeed, modulation of the topography and the Young's modulus of 3D scaffolds has been found to regulate MSC morphology, proliferation, and multi‐lineage differentiation potential . For instance, MSC proliferation and osteogenic differentiation were promoted on stiffer (40 kPa) 3D matrices compared to softer matrices (6 kPa) in a similar manner to 2D cultures .…”

Section: Msc Organization and Properties In 3d Culturesmentioning

confidence: 99%

Regulation of mesenchymal stem cell 3D microenvironment: From macro to microfluidic bioreactors

Sart

Agathos

et al. 2015

Biotechnology Journal

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Human mesenchymal stem cells (hMSCs) have emerged as an important cell type in cell therapy and tissue engineering. In these applications, maintaining the therapeutic properties of hMSCs requires tight control of the culture environments and the structural cell organizations. Bioreactor systems are essential tools to achieve these goals in the clinical-scale expansion and tissue engineering applications. This review summarizes how different bioreactors provide cues to regulate the structure and the chemico-mechanical microenvironment of hMSCs with a focus on 3D organization. In addition to conventional bioreactors, recent advances in microfluidic bioreactors as a novel approach to better control the hMSC microenvironment are also discussed. These advancements highlight the key role of bioreactor systems in preserving hMSC's functional properties by providing dynamic and temporal regulation of in vitro cellular microenvironment.

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“…A few studies have focused on replicating native tissue to direct cell behaviour [161][162][163][164]. For example, it was shown that by replicating the tendon micro-environment ( Figure 11A), mesenchymal stem cells (MSCs) can be guided to differentiate towards a tenogenic phenotype [162].…”

Section: Replication Of Native Tissue To Direct Cell Behaviourmentioning

confidence: 99%

Natural Architectures for Tissue Engineering and Regenerative Medicine

Honig

Vermeulen

Zadpoor

et al. 2020

JFB

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The ability to control the interactions between functional biomaterials and biological systems is of great importance for tissue engineering and regenerative medicine. However, the underlying mechanisms defining the interplay between biomaterial properties and the human body are complex. Therefore, a key challenge is to design biomaterials that mimic the in vivo microenvironment. Over millions of years, nature has produced a wide variety of biological materials optimised for distinct functions, ranging from the extracellular matrix (ECM) for structural and biochemical support of cells to the holy lotus with special wettability for self-cleaning effects. Many of these systems found in biology possess unique surface properties recognised to regulate cell behaviour. Integration of such natural surface properties in biomaterials can bring about novel cell responses in vitro and provide greater insights into the processes occurring at the cell-biomaterial interface. Using natural surfaces as templates for bioinspired design can stimulate progress in the field of regenerative medicine, tissue engineering and biomaterials science. This literature review aims to combine the state-of-the-art knowledge in natural and nature-inspired surfaces, with an emphasis on material properties known to affect cell behaviour.

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Efficient myogenic commitment of human mesenchymal stem cells on biomimetic materials replicating myoblast topography

Cited by 23 publications

References 47 publications

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

Substrate topography: A valuable in vitro tool, but a clinical red herring for in vivo tenogenesis

Regulation of mesenchymal stem cell 3D microenvironment: From macro to microfluidic bioreactors

Natural Architectures for Tissue Engineering and Regenerative Medicine

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