Regulation of mesenchymal stem cell 3D microenvironment: From macro to microfluidic bioreactors

Sart, Sébastien; Agathos, Spiros N.; Li, Yan; Ma, Teng

doi:10.1002/biot.201500191

Cited by 52 publications

(34 citation statements)

References 125 publications

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“…Recent advances in the design of hydrogel biomaterials with tunable physicochemical features and fabrication techniques (e.g., micropatterning, bioprinting, and microfluidics etc.) have offered a versatile toolbox for engineering 3D spatiotemporal mechanical microenvironments of stem cells in vitro.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate

et al. 2018

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Stem cells hold great promise for widespread biomedical applications, for which stem cell fate needs to be well tailored. Besides biochemical cues, accumulating evidence has demonstrated that spatiotemporal biophysical cues (especially mechanical cues) imposed by cell microenvironments also critically impact on the stem cell fate. As such, various biomaterials, especially hydrogels due to their tunable physicochemical properties and advanced fabrication approaches, are developed to spatiotemporally manipulate biophysical cues in vitro so as to recapitulate the 3D mechanical microenvironment where stem cells reside in vivo. Here, the main mechanical cues that stem cells experience in their native microenvironment are summarized. Then, recent advances in the design of hydrogel materials with spatiotemporally tunable mechanical properties for engineering 3D the spatiotemporal mechanical microenvironment of stem cells are highlighted. These in vitro engineered spatiotemporal mechanical microenvironments are crucial for guiding stem cell fate and their potential biomedical applications are subsequently discussed. Finally, the challenges and future perspectives are presented.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate

et al. 2018

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“…The advances in microfluidic technology brought great progresses in the field of dynamic in vitro models, mainly regarding the spatiotemporal control of gradients and the introduction of individual or combination of factors with low volumes and low cell suspension density requirements (Sart et al, 2016;Sun et al, 2012b;Tatárová et al, 2016). Recently, Marturano-Kruik et al (2018) have developed a perivascular model containing ECs and MSCs seeded on a bone matrix, forming a bone perivascular nicheon-a-chip, which allows following slow-cycling metastatic cancer cells in a BM niche.…”

Section: Microfluidic Chipsmentioning

confidence: 99%

Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro

Pereira¹,

Trivanović²,

Herrmann³

2019

eCM

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Mesenchymal stem/stromal cells (MSCs) are an essential element of most modern tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. The present comprehensive review extensively discusses the microenvironmental cues that influence MSC phenotype and function in health and disease-including cellular, chemical and physical interactions. The most recent and relevant illustrative examples of novel bioengineering approaches to mimic biological, chemical, and mechanical microenvironmental signals present in the native MSC niche are summarized, with special emphasis on the forefront techniques to achieve biochemical complexity and dynamic cultures. In particular, the skeletal MSC niche and applications focusing on the bone regenerative potential of MSC are addressed. The aim of the review was to recognize the limitations of the current MSC niche in vitro models and to identify potential opportunities to fill the bridge between fundamental science and clinical application of MSCs.

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“…tension [56] and establishing dynamic cultures such as porous scaffolds, spheroids, or their combination, as well as encapsula-325 tion in hydrogels or even inside bioreactors [57]. Although diverse studies examined the secreted factor production of different cell types, most of recently published reports focus on MSCs due to their widespread preclinical use for tissue regeneration.…”

Section: Modulation Of Cell Secretome Profilementioning

confidence: 99%

“…Bioreactor systems can provide an interactive 3D microenvironment through the regulation of the spatial distribution of macromolecules and mechanical cues. Therefore, they have the capacity to recreate the interactions between MSCs and their microenvironment [57,68]. Mechanical forces are capable of shaping MSCs' fate and bioreactors provide a tool to study the cellular response to mechanical stimulation under controlled conditions [69].…”

Section: Hypoxiamentioning

confidence: 99%

Cell secretome based approaches in Parkinson’s disease regenerative medicine

Marques

Marote

Mendes-Pinheiro

et al. 2018

Expert Opinion on Biological Therapy

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Introduction: The available therapeutic strategies for Parkinson's disease (PD) rely only on the amelioration of the symptomatology of the disease, lacking neuroprotection or neuroregeneration capacities. Therefore, the development of disease modifying strategies is extremely important for the management of PD in the long term. Areas covered: In this review, the authors provide an overview of the current therapeutic approaches for PD and the emerging use of stem cell transplantation as an alternative. Particularly, the use of the secretome from mesenchymal stem cells (MSCs), as well as some methodologies used for the modulation of their paracrine signaling, will be discussed. Indeed, there is a growing body of literature highlighting the use of paracrine factors and vesicles secreted from different cell populations, for this purpose. Expert opinion: Secretome from MSCs has shown its potential as a therapy for PD. Nevertheless, in the coming years, research should focus in several key aspects to enable the translation of this strategy from the bench to the bedside.

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Regulation of mesenchymal stem cell 3D microenvironment: From macro to microfluidic bioreactors

Cited by 52 publications

References 125 publications

3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate

3D Spatiotemporal Mechanical Microenvironment: A Hydrogel‐Based Platform for Guiding Stem Cell Fate

Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro

Cell secretome based approaches in Parkinson’s disease regenerative medicine

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