Textile materials have become one of the functional needs of human beings and their multifunctional properties are highly demanded. Linen has been gaining rising interest and the multifunctional modification of this fiber can produce value-added textile products. In the present study, linen fabric was modified using natural dyeing with a combination of henna and copper sulphate. The resulting colored substrate was characterized using FTIR, TGA and SEM techniques. The color values of the dyed samples, for the various combinations of mordant and dye concentrations, were evaluated, along with the functional properties, such as antioxidant activity, antibacterial action and UV protection. The modified linen showed wash-fast coloration and displayed efficient protection against bacteria, UV light and free radicals. The multifunctional properties were retained after 20 washes. The henna-dyed linen can be claimed as a green multifunctional textile material that can be used for enhanced protection.