Efficient identification of proteins from ovaries and hepatopancreas of the unsequenced edible crab, Cancer pagurus, by mass spectrometry and homology-based, cross-species searching

Ward, Deborah; Sefton, Elaine M.; Prescott, Mark; Webster, S. G.; Wainwright, Geoff; Rees, Huw H.; Fisher, Michael J.

doi:10.1016/j.jprot.2010.07.008

Cited by 13 publications

(8 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Unlike vertebrate hemoglobins, HCYs are cellindependent, and are solely suspended in the crayfish haemolymph [73]. This means that the HCYs can be directly excreted from the hepatopancreas, where they are synthesised, to the crayfish haemolymph, without damage to the organism [74,75]. On the other hand, proPO must be transported to the infection site and incorporated in the granules of semi-granular and granular haemocytes (blood cells) [13,60].…”

Section: Activation Of Prophenoloxidase Cascadementioning

confidence: 99%

Comparative transcriptome analysis of noble crayfish and marbled crayfish immune response to Aphanomyces astaci challenges

Boštjančić

Francesconi

Rutz

et al. 2021

Preprint

View full text Add to dashboard Cite

Introduction of invasive North American crayfish species and their pathogen Aphanomyces astaci has significantly contributed to the decline of European freshwater crayfish populations. In this study, noble crayfish, a susceptible native European species, and marbled crayfish, an invasive disease-resistant species, were challenged with haplogroup A (low virulence) and haplogroup B (high virulence) strain of A. astaci. Hepatopancreatic tissue was isolated 3 and 21 days post-challenge. Our results revealed strong up-regulation in expression levels of the prophenoloxidase cascade immune-related genes in the haplogroup B challenged noble crayfish 3 days post-challenge. In the marbled crayfish, we observed an up-regulation of immune system relevant genes (DSCAM, AP, ALFs, CTLs and hemocyanin) 3 days post-challenge. This response highlights the marbled crayfish capability of building the immune tolerance. Furthermore, we successfully characterised several novel immune related gene groups in both crayfish species, contributing to our current understanding of crayfish immune related genes landscape.

show abstract

Section: Activation Of Prophenoloxidase Cascadementioning

confidence: 99%

Comparative transcriptome analysis of noble crayfish and marbled crayfish immune response to Aphanomyces astaci challenges

Boštjančić

Francesconi

Rutz

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Cancer pagurus, commonly known as edible or brown crab, is a species of crab living in the North Sea, North Atlantic Ocean, Mediterranean Sea, and Black Sea (Markl et al 1979;Markl and Kempter 1981;Stöcker et al 1988;Rochu and Fine 1984;Ward et al 2010). Proteome maps of the hepatopancreas of crustacean C. pagurus were performed by 2D-PAGE, and vitellogenin (yolk protein), several subunits of hemocyanin, cryptocyanin, ferritin, and calreticulin were identified (Ward et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Proteome maps of the hepatopancreas of crustacean C. pagurus were performed by 2D-PAGE, and vitellogenin (yolk protein), several subunits of hemocyanin, cryptocyanin, ferritin, and calreticulin were identified (Ward et al 2010). Hemocyanins are coppercontaining respiratory proteins, dissolved in concentrations up to 120 mg/ml in the hemolymph of molluscs and arthropods, including Crustacea, Chelicerata, Myriapoda, and some species of insects (Hagner-Holler et al 2004;Sánchez et al 1998;van Holde and Miller 1995;Chen et al 2007;Mičetic et al 2010).…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Structure and Characterization of Cancer pagurus Hemocyanin

et al. 2014

View full text Add to dashboard Cite

“…HC is a major component of haemolymph, which is an efficient biological defence weapon utilized in invertebrate innate immunity (Coates & Nairn, ; Destoumieux‐Garzón et al, ; Qiao et al, ; Zhang et al, ). HC can exert respiratory functions in shrimp (Cheng, Liu, & Kuo, ; Coates & Nairn, ; Zhou et al, ), and can be synthesized in crustacean hepatopancreas (Lee, Lee, & Söderhäll, ; Ward et al, ). It was found that in the case of hypoxia, shrimp with lower HC concentration has a greater need to synthesize more HC to maintain oxygen delivery (McMahon, ; Tommerdahl, Burnett, & Burnett, ).…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary supplementation of Astragalus membranaceus , Codonopsis pilosula , and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp ( Litopenaeus vannamei )

et al. 2019

View full text Add to dashboard Cite

Pacific white shrimp is the major farmed shrimp species in the world. It is known to be very sensitive to the environmental and management changes such as intensification, which is one of the primary necessities to increase shrimp production, but represents a stressful condition, and needs to be managed properly to diminish its negative effects in aquaculture. In this study, juvenile Pacific white shrimp were fed diets supplementing with equal quantities of Astragalus membranaceus, Codonopsis pilosula and Glycyrrhiza uralensis extracts in different concentrations (0, 1.0, 2.0 and 3.0 g/kg) for 8 weeks. We subsequently estimated the effects of supplemented diets on growth performance, haematological parameters, and histological changes of hepatopancreas. Results revealed that the supplemented mixture had no benefits on growth and survival rate, but had favourable effects on haemocyte count, number of granulocytes, and hemocyanin concentration in the haemolymph (p > 0.05). We observed that R cells and B cells were increased in hepatopancreatic tubules of shrimps fed on supplemented diets. Besides, a diet containing 2.0 g/kg of extract mixture maintained the decreased levels of alanine aminotransferase (p < 0.05) and aspartate aminotransferase (p > 0.05). Among the used concentrations, 2.0 g/kg seemed to be the most suitable concentration. K E Y W O R D S Astragalus membranaceus, Codonopsis pilosula, Glycyrrhiza uralensis, histology, immune response, Litopenaeus vannamei How to cite this article: Prathomya P, Hussein HA, Tran NT, et al. Effects of dietary supplementation of Astragalus membranaceus, Codonopsis pilosula, and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp (Litopenaeus vannamei). Aquac Res.

show abstract

Efficient identification of proteins from ovaries and hepatopancreas of the unsequenced edible crab, Cancer pagurus, by mass spectrometry and homology-based, cross-species searching

Cited by 13 publications

References 34 publications

Comparative transcriptome analysis of noble crayfish and marbled crayfish immune response to Aphanomyces astaci challenges

Comparative transcriptome analysis of noble crayfish and marbled crayfish immune response to Aphanomyces astaci challenges

RETRACTED ARTICLE: Structure and Characterization of Cancer pagurus Hemocyanin

Effects of dietary supplementation of Astragalus membranaceus , Codonopsis pilosula , and Glycyrrhiza uralensis extract mixture on growth performance, haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp ( Litopenaeus vannamei )

Contact Info

Product

Resources

About