Efficient formation of cell spheroids using polymer nanofibers

Abstract

Spheroid culture has been used for suspension cultures of anchorage-dependent cells. In this study, we developed a new method for the suspension cultures of anchorage-dependent animal cells using polymer nanofibers. Poly(lactic-co-glycolic acid) nanofibers (785 nm in average fiber-diameter, 88 μm in average fiber-length) fabricated by the electrospinning method were added to each suspension culture of human embryonic kidney 293 cells and human dermal fibroblasts. As compared to no addition of nanofibers to the suspension cultures, nanofibers enhanced cell spheroid formation, thereby reducing cell death resulting from a lack of cell adhesion. Efficient formation of spheroids in the presence of polymer nanofibers may be useful for the suspension cultures of anchorage-dependent cells.

Reversing charges or how to improve Wharton's jelly mesenchymal stem cells culture on polyelectrolyte multilayer films

“…Growing cells as three dimensioned architectures has been described in many research fields, many teams aimed to achieve this type of nodules from different cells. Human dermal fibroblasts were at the origin of these nodules, as described in the work of Shin et al [42]. Others like Yoon et al, Cheng et al and other colleagues stimulated human adipose-derived stem cells to form these spheres [43][44][45].…”

Engineering Multi‐Cellular Spheroids for Tissue Engineering and Regenerative Medicine

“…For example, composite spheroids of embryonic kidney cells or dermal fibroblasts mixed with PLGA nanofiber particles have been reported to reduce spheroid formation time to within 24 h with the resulting spheroids maintaining viability over 3 days. [ 136 ] Similarly, the hypoxia inside of fibroblasts/ECM‐mimicking‐nanofiber composite spheroids was reduced, and the proliferation level and cell viability were well maintained for 14 days compared to that from cell‐only spheroid, despite their relatively larger size (over 500 µm). [ 137 ] Primary hepatocyte spheroids with poly(styrene‐ co ‐maleic acid) nanofiber particles exhibited a higher clearance rate of various drugs, including tolbutamide, midazolam, and acetaminophen, compared to nanofiber‐free spheroids, indicating that presence of nanofibers within the spheroids partially mitigated compaction‐mediated diffusion limitations.…”

A 3D Fibrous Scaffold Inducing Tumoroids: A Platform for Anticancer Drug Development

“…The electrospinning conditions can be adjusted to produce fibrous scaffolds tailored for specific cell culture needs [35], [39]. Cancer cells have also been induced to form spheroids on electrospun galactosylated fibers [40] and 3D scaffolds [15]. However, 3D fibrous scaffold (3DFS)-induced tumor spheroids have been poorly characterized and it is not known whether these spheroids resemble in vivo tumors.…”