Efficient enzymatic saccharification of alkaline and ionic liquid-pretreated bamboo by highly active extremozymes produced by the co-culture of two halophilic fungi

Zhao, Bo; Rasheed, Haroon Al; Imran, Ali; Hu, Shanglian

doi:10.1016/j.biortech.2020.124115

Cited by 31 publications

(11 citation statements)

References 33 publications

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“…These may be due to the components of the fungal cell walls. Recently, Zhao et al (2021) studied the co-culture of Aspergillus flavus and Aspergillus penicillioides as a potent method for hydrolytic enzyme production, and observed the maximum production of carboxy methyl cellulase, β-xylosidase, and xylanase from the co-culture of these fungi compared to the production from the separately cultivated fungi. Aspergillus flavus, A. fumigatus, and the dual culture were incubated at different temperatures to detect the productivity of various enzymes.…”

Section: Resultsmentioning

confidence: 99%

Induction of hydrolytic enzyme production and antibiosis via a culture of dual fungal species isolated from soil rich with the residues of woody plants in Saudi Arabia

Al-Rajhi

Alharbi

Yahya

et al. 2022

BioRes

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Inducing hydrolytic enzymes production and antibiosis is an attractive process for industrial applications. The approach can be used to repress pathogenic microorganisms. Using a dual culture of Aspergillus fumigatus and Aspergillus flavus, the activities of cellulase, polygalacturonase, chitinase, β-1,3-glucanases, protease, xylanase, and β-glucosidase were 1.78, 3.87, 2.98, 2.79, 6.91, 2.89 U, and 1.43 U∙mg-1 of protein. Meanwhile, the activities were 0.87, 2.78, 0.58, 1.69, 4.45, 2.06, and 0.89 U.mg-1 of protein for A. flavus alone and 0.98, 2.98, 0.87, 1.89, 4.98, 2.58, and 0.91 U.mg-1 of protein for A. fumigatus alone. The cellulase, polygalacturonase, and chitinase activities were studied at different temperatures; 40 °C and 50 °C, which were better temperatures than 20 °C in terms of enzymes activity for A. fumigatus and the dual culture compared to A. flavus. The highest antimicromial activity was observed using the dual fungal culture, where the inhibition zones were 3.13, 3.47, 2.27, 1.77, 1.03, and 2 mm compared with A. fumigatus alone or A. flavus alone, against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Aspergillus fumigatus, Mucor circinelloides, and Fusarium moniliforme, respectively.

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Section: Resultsmentioning

confidence: 99%

Induction of hydrolytic enzyme production and antibiosis via a culture of dual fungal species isolated from soil rich with the residues of woody plants in Saudi Arabia

Al-Rajhi

Alharbi

Yahya

et al. 2022

BioRes

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“…In this study, the optimum temperature for the growth and production of salt tolerant enzymes by Aspergillus niger isolate L2S1 was found to be 30°C. Bellaouchi et al [59] inferred that the best enzyme production in Aspergillus species is obtained at 30°C, however, Zhao et al [60] reported the optimum production of salt tolerant cellulase by obligate halophilic Aspergillus flavus strain 6830 at 35°C using solid-state fermentation (SSF). The assayed enzyme activity in this study was considerably affected by pH.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Activities of Halotolerant and Halophilic Fungi Isolated from Iko River Estuary, South-South Nigeria

Ufot

Antia²,

Umoh³

et al. 2022

JABB

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Aim: Fungi that can tolerate unfavorable conditions are a potential source of stable bioactive metabolites that can be used for various industrial processes. Therefore this study aimed to isolate halotolerant and halophilic fungi from the Iko river estuary and screen them for three industrially important enzymes: amylase, cellulase, and mannanase. Place and Duration of Study: Department of Microbiology, Akwa Ibom State University, Nigeria, between September 2021 and August 2022. Methodology: Five sediment-dwelling fungi isolated from three locations along the Iko river estuary were assayed for their halotolerance on PDA containing different NaCl concentrations while their amylolytic, cellulolytic, and mannolytic activities were evaluated on agar plates containing 10% NaCl. The highest producer of the screened enzymes was identified using molecular techniques and further subjected to various submerged fermentation conditions to optimize the production of salt tolerant amylase, cellulase, and mannanase. Results: All isolates were halotolerant, best adapted to a salinity of 5% NaCl (w/v), and capable of secreting at least one of the three tested enzymes in 10% NaCl (w/v). Aspergillus niger isolate L2S1 had the highest enzymatic index among other isolates thus requiring optimization. The optimum production of amylase, cellulase, and mannanase was obtained at an incubation temperature of 30oC, initial pH of 6.0, and NaCl concentration of 5% (w/v). Out of the tested agro-wastes, wheat bran was most suited for the production of amylase, corncob was optimum for cellulase production, and maximum mannanase yield was obtained using copra meal. Amylase and cellulase production was optimal at 96 hours of incubation, whereas mannanase required 144 hours of fermentation to reach its maximum activity. Conclusion: Aspergillus niger isolate L2S1 was capable of utilizing different carbon sources including cheap agro-residues as substrates for the production of halostable amylase, cellulase, and mannanase.

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“…Microorganisms belonging to the same genus have shown better compatibility with each other, hence are extensively used to produce various metabolites. This can be inferred from studies on pair of microorganisms such as Bacillus cereus and Bacillus thuringiensis, Aspergillus niger MS23, and Aspergillus terreus MS105, Clostridium thermocellum ATCC 27405 and Clostridium beijerinckii ATCC 51743, Aspergillus flavus and Aspergillus penicillioides [1][2][3][4].…”

Section: Co-culturementioning

confidence: 99%

A Critical Look at Bioproducts Co-cultured Under Solid State Fermentation and Their Challenges and Industrial Applications

Prabhu

Bhat

et al. 2022

Waste Biomass Valor

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The production of bioproducts from microorganisms is a common practice in many industries for a long time now. In recent years, studies have proved that co-culturing microorganisms increase the yield of products by synergistically degrading the solid substrate in comparison with individual cultures. The review highlights the benefits of co-culturing microorganisms using solid state fermentation (SSF) to achieve higher productivity. Filamentous fungi of genus Trichoderma, Penicillium, and Aspergillus are extensively studied and used for co-culturing and mixed culturing under SSF. Co-cultured microorganisms are beneficial because of the synergistic expression of metabolic pathways of all the microorganisms. Co-culture enables combined metabolic activity at optimal process conditions for better utilization of substrates. Depending on the nature of the process and microorganism, bioreactors are designed and operated. This review mentions various purification methods that are used to improve the purity of the products obtained. The strengths and weaknesses of various bioreactors and their effect on the microorganisms used are explained in detail. This review also identifies the challenges of co-culturing microorganisms and analyses the diverse set of fields in which SSF finds its applications. Graphical Abstract

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Efficient enzymatic saccharification of alkaline and ionic liquid-pretreated bamboo by highly active extremozymes produced by the co-culture of two halophilic fungi

Cited by 31 publications

References 33 publications

Induction of hydrolytic enzyme production and antibiosis via a culture of dual fungal species isolated from soil rich with the residues of woody plants in Saudi Arabia

Induction of hydrolytic enzyme production and antibiosis via a culture of dual fungal species isolated from soil rich with the residues of woody plants in Saudi Arabia

Enzymatic Activities of Halotolerant and Halophilic Fungi Isolated from Iko River Estuary, South-South Nigeria

A Critical Look at Bioproducts Co-cultured Under Solid State Fermentation and Their Challenges and Industrial Applications

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