Efficient enrichment of total flavonoids from Pteris ensiformis Burm. extracts by macroporous adsorption resins and in vitro evaluation of antioxidant and antiproliferative activities

et al. 2021

Molecules

Pinus koraiensis nut-coated film is a kind of by-product of nut processing, which has been shown to contain flavonoids, polyphenols, and other substances that can be used to produce natural antioxidant extracts. In this study, response surface methodology (RSM) was used to optimize the extraction process of flavonoids of P. koraiensis nut-coated film (PNF), and macroporous resin HPD600 was used to purify PNF (P-PNF). Its antioxidant activity was examined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging capacity, oxygen free radical absorption capacity (ORAC), total oxygen radical capture (TRAP), and iron ion reduction capacity. Under the ideal extraction conditions comprising a cellulase dosage of 90 U/g, a material/liquid ratio of 1:20 (g/mL), and an extraction time of 2 h, the PNF yield was 3.37%. Purification conditions were sample concentration of 2.0 mg/mL, pH of 5, water washing volume of 3 bed volume (BV), eluent ethanol concentration of 50%, and volume of 2 BV. The P-PNF recovery was 84.32%, and purity increased from 33.80% to 61.70%. Additionally, P-PNF showed increased antioxidant activity compared to PNF. Cumulatively, this study obtained the optimal values for the process parameters in order to achieve the maximum rates of extraction of PNF for economically optimal production at an industrial scale.

Section: Introductionmentioning

confidence: 99%

Optimization of Enzyme-Assisted Extraction and Purification of Flavonoids from Pinus koraiensis Nut-Coated Film and Antioxidant Activity Evaluation

Zhang

et al. 2021

Molecules

“…Accordingly, the HPD-600 macroporous resin was selected for further purifying ASTFs due to its superior adsorption and desorption among the six resins. HPD-600 was more suitable to enrich flavonoids based on the fact that HPD-600 resin is a polar resin, and the flavonoids was polar compounds; it involves a principle “like dissolve like” [ 16 ]. And there are more hydrogen bonds and stronger van der Waals interactions between HPD-600 resin and Acanthopanax senticosus total flavonoids.…”

Section: Resultsmentioning

confidence: 99%

“…Adsorption and desorption are not only affected by polarity but also by the surface area and pore size of the macroporous resin. The use of macroporous resin adsorption as an effective method to purify total flavonoids has been reported in the separation and purification of flavonoids, such as Sophora tonkinensis Gagnep [ 13 ], Ginkgo biloba leaves [ 14 ], Rhizoma smilacis glabrae [ 15 ], and Pteris ensiformis Burm [ 16 ]. This experiment enriches the total flavonoids of Acanthopanax senticosus and understands its adsorption mechanism through the selection of macroporous resin and single factor investigation, studying adsorption kinetics, adsorption isotherms and thermodynamics, etc.…”

Section: Introductionmentioning

confidence: 99%

Adsorption and Desorption Characteristics of Total Flavonoids from Acanthopanax senticosus on Macroporous Adsorption Resins

Chu

et al. 2021

Molecules

We examined the application of six different resins with the aim of selecting a macroporous resin suitable for purifying Acanthopanax senticosus total flavonoids (ASTFs) from Acanthopanax senticosus crude extract (EAS) by comparing their adsorption/desorption capacities, which led to the selection of HPD-600. Research on the adsorption mechanism showed that the adsorption process had pseudo-second-order kinetics and fit the Freundlich adsorption model. Moreover, the analysis of thermodynamic parameters indicated that the adsorption process is spontaneous and endothermic. The optimal conditions for purification of ASTFs were determined as sample pH of 3, 60% ethanol concentration, and 3 BV·h−1 flow rate, for both adsorption and desorption, using volumes of 2.5 and 4 BV, respectively. The application of macroporous resin HPD-600 to enrich ASTFs resulted in an increase in the purity of total flavonoids, from 28.79% to 50.57%. Additionally, the antioxidant capacity of ASTFs was higher than that of EAS, but both were lower than that of L-ascorbic acid. The changes in ASTFs compositions were determined using ultra-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS), with the results illustrating that the levels of seven major flavonoids of ASTFs were increased compared to that in the crude extract.

“…Excessive reactive oxygen/nitrogen species (ROS-RNS) production disturbs the antioxidant/pro-antioxidant system and contributes to oxidative stress, which contributes towards development in humans of diabetes mellitus, neurodegenerative disorders, cardiovascular diseases, cancer, etc. [42,43], with studies pointing to a strong correlation between bioactive compounds and antioxidant activities [44,45]. Consequently, in this study the flavonoid extract was screened for antioxidant activities by different assays (•DPPH, ABTS ■ and hydroxyl radicals).…”

Section: Discussionmentioning

confidence: 99%

Ultrasound-microwave assisted extraction of flavonoid compounds from Eucommia ulmoides leaves and an evaluation of their antioxidant and antibacterial activities

Peng²,

Wang³

et al. 2020

Arch biol sci (Beogr)

Ultrasound/microwave assisted extraction (UMAE) of flavonoid compounds from Eucommia ulmoides leaves was studied and the extraction conditions were optimized by the Plackett-Burman design (PBD) method combined with the Box-Behnken design (BBD). The antioxidant and antibacterial activities of the flavonoid extract were investigated. The results show that the optimal conditions were an ethanol concentration of 41%, microwave power of 178 W and an ultrasound extraction time of 26 min. Under these conditions, the yield of the flavonoid compounds was 2.454%±0.230%, which was higher than that after direct solvent extraction, ultrasound extraction and microwave extraction. The results of in vitro antioxidant assays showed that the flavonoid extract had scavenging capacity for DPPH, ABTS and hydroxyl radicals, with corresponding IC 50 values of 30.76 mg/L, 21.09 mg/L, 248.4 mg/L, respectively. In addition, this extract exhibited strong antibacterial activity on Escherichia coli, Staphylococcus aureus and Salmonella typhimurium.