Efficient Delivery of mRNA Using Crosslinked Nucleic Acid Nanogel as a Carrier

Huang, Xiangang; Zheng, Ru; Ding, Fei; Yang, Jiapei; Xie, Miao; Fan, Haosen; Li, Jing; Feng, Jing; Zhu, Xinyuan; Zhang, Chuan

doi:10.1021/acsmaterialslett.0c00375

Cited by 32 publications

(42 citation statements)

References 43 publications

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“…[207] 4) Nucleic acids with unique programmability, diverse chemical modification, as well as specific spatial structures and functions, make it an ideal carrier for drug delivery. At present, NAHs as a promising controllable delivery system has been applied to load several types of cargoes, such as nucleic acid drugs, small-molecule drugs, and other specific biomaterials or tools, which mainly perform their functions in gene therapy, [210][211][212][213] immunotherapy, [43,[214][215][216][217] and chemotherapy. [86,208,[218][219][220][221] In recent years, with deep investigations of the microenvironment alteration near different lesions, the treatment protocols with smart administration systems have made great progress in the delivery of nucleic acid drugs, chemodrugs, and certain small drugs.…”

Section: Controllable Drug Delivery and Targeted Therapymentioning

confidence: 99%

“…The other strategy was to deliver Cas 9 protein by integrating its code information into message RNA (mRNA) which plays an important role in regulating therapeutic protein expression in the target cell for the diseases treatment and provides solid assurance of stability for the delivery system in the absence of nuclease. Huang et al [212] followed this strategy and achieved the first delivery of the functional mRNA by NAHs. According to a similar route as the previous study, the polythymine (poly T)-g-PCL as a DNA brush synthesized first and hybridized with the polyadenine (poly A) tail of the Cas 9-encoded mRNA.…”

Section: Controllable Drug Delivery and Targeted Therapymentioning

confidence: 99%

“…[ 207 ] 4) Nucleic acids with unique programmability, diverse chemical modification, as well as specific spatial structures and functions, make it an ideal carrier for drug delivery. At present, NAHs as a promising controllable delivery system has been applied to load several types of cargoes, such as nucleic acid drugs, small‐molecule drugs, and other specific biomaterials or tools, which mainly perform their functions in gene therapy, [ 210 , 211 , 212 , 213 ] immunotherapy, [ 43 , 214 , 215 , 216 , 217 ] and chemotherapy. [ 86 , 208 , 218 , 219 , 220 , 221 ]…”

Section: Applications Of Smart and Functionalized Nahsmentioning

confidence: 99%

Section: Applications Of Smart and Functionalized Nahsmentioning

confidence: 99%

“…successively reported several different nucleic acid nanogel‐based delivery systems for gene regulation and genome editing. [ 210 , 211 , 212 , 213 ] Xue et al. [ 210 ] directly applied DNA tetrahedron as building blocks and small interfering RNA (siRNA) as cross‐links to fabricate all‐nucleic acid nanogels for the target gene knockdown.…”

Section: Applications Of Smart and Functionalized Nahsmentioning

confidence: 99%

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Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

et al. 2021

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Nucleic acid-based hydrogels that integrate intrinsic biological properties of nucleic acids and mechanical behavior of their advanced assemblies are appealing bioanalysis and biomedical studies for the development of new-generation smart biomaterials. It is inseparable from development and incorporation of novel structural and functional units. This review highlights different functional units of nucleic acids, polymers, and novel nanomaterials in the order of structures, properties, and functions, and their assembly strategies for the fabrication of nucleic acid-based hydrogels. Also, recent advances in the design of multifunctional and stimuli-responsive nucleic acid-based hydrogels in bioanalysis and biomedical science are discussed, focusing on the applications of customized hydrogels for emerging directions, including 3D cell cultivation and 3D bioprinting. Finally, the key challenge and future perspectives are outlined.

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Section: Controllable Drug Delivery and Targeted Therapymentioning

confidence: 99%

Section: Controllable Drug Delivery and Targeted Therapymentioning

confidence: 99%

“…[ 207 ] 4) Nucleic acids with unique programmability, diverse chemical modification, as well as specific spatial structures and functions, make it an ideal carrier for drug delivery. At present, NAHs as a promising controllable delivery system has been applied to load several types of cargoes, such as nucleic acid drugs, small‐molecule drugs, and other specific biomaterials or tools, which mainly perform their functions in gene therapy, [ 210 , 211 , 212 , 213 ] immunotherapy, [ 43 , 214 , 215 , 216 , 217 ] and chemotherapy. [ 86 , 208 , 218 , 219 , 220 , 221 ]…”

Section: Applications Of Smart and Functionalized Nahsmentioning

confidence: 99%

Section: Applications Of Smart and Functionalized Nahsmentioning

confidence: 99%

Section: Applications Of Smart and Functionalized Nahsmentioning

confidence: 99%

See 3 more Smart Citations

Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

et al. 2021

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Rational Design of DNA Hydrogels Based on Molecular Dynamics of Polymers

Li,

Chen,

Zhou

et al. 2023

Advanced Materials

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In recent years, DNA has emerged as a fascinating building material to engineer hydrogels due to its excellent programmability, which has gained considerable attention in biomedical applications. Understanding the structure‐property relationship and underlying molecular determinants of DNA hydrogel is essential to precisely tailor its macroscopic properties at molecular level. In this review, we highlight the rational design principles of DNA molecular networks based on molecular dynamics of polymers on the temporal scale, which can be engineered via the backbone rigidity and crosslinking kinetics. By elucidating the underlying molecular mechanisms and theories, we aim to provide a comprehensive overview of how the tunable DNA backbone rigidity and the crosslinking kinetics lead to desirable macroscopic properties of DNA hydrogels, including mechanical properties, diffusive permeability, swelling behaviors, and dynamic features. Furthermore, we also discuss how the tunable macroscopic properties make DNA hydrogels promising candidates for biomedical applications, such as cell culture, tissue engineering, bio‐sensing, and drug delivery.This article is protected by copyright. All rights reserved

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Cellular Trafficking of Nanotechnology‐Mediated mRNA Delivery

Huang,

Deng,

Wang

et al. 2023

Advanced Materials

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Messenger RNA (mRNA)‐based therapy has emerged as a powerful, safe, and rapidly scalable therapeutic approach that involves technologies for both mRNA itself and the delivery vehicle. Although there are some unique challenges for different applications of mRNA therapy, a common challenge for all mRNA therapeutics is the transport of mRNA into the target cell cytoplasm for sufficient protein expression. This review is focused on the behaviors at the cellular level of nanotechnology‐mediated mRNA delivery systems, which have not been comprehensively reviewed yet. First, the four main therapeutic applications of mRNA are introduced, including immunotherapy, protein replacement therapy, genome editing, and cellular reprogramming. Second, common types of mRNA cargos and mRNA delivery systems are summarized. Third, strategies to enhance mRNA delivery efficiency during the cellular trafficking process are highlighted, including accumulation to the cell, internalization into the cell, endosomal escape, release of mRNA from the nanocarrier, and translation of mRNA into protein. Finally, the challenges and opportunities for the development of nanotechnology‐mediated mRNA delivery systems are presented. This review can provide new insights into the future fabrication of mRNA nanocarriers with desirable cellular trafficking performance.

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Efficient Delivery of mRNA Using Crosslinked Nucleic Acid Nanogel as a Carrier

Cited by 32 publications

References 43 publications

Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

Smart and Functionalized Development of Nucleic Acid‐Based Hydrogels: Assembly Strategies, Recent Advances, and Challenges

Rational Design of DNA Hydrogels Based on Molecular Dynamics of Polymers

Cellular Trafficking of Nanotechnology‐Mediated mRNA Delivery

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