Efficient Degradation of High-Molecular-Weight Hyaluronic Acid by a Combination of Ultrasound, Hydrogen Peroxide, and Copper Ion

Abstract

Hyaluronic acid (HA) was depolymerized by a combination of ultrasound, hydrogen peroxide and copper ion. The structures of high-molecular-weight hyaluronic acid (HMW-HA) and low-molecular-weight hyaluronic acid (LMW-HA) were determined by Fourier transform infrared (FTIR) spectroscopy, circular dichroism (CD) spectroscopy, and UV-VIS absorption spectroscopy. The degradations of HMW-HA using a physical method, a chemical method, and a combination of physical and chemical method were compared. The results show that HA can be effectively degraded by a combinatorial method involving ultrasound, hydrogen peroxide, and copper ion. Under the degradation conditions of 50 mM H2O2, 5.0 μM CuCl2, 160 W, pH 4.0, and reaction at 50 °C for 30 min, the content of glucuronic acid was 36.56%, and the yield of LMW-HA was 81.71%. The FTIR, CD, and UV-VIS absorption spectra of HA did not change with the decrease in molecular weight, indicating that the structure of HA remained intact during the degradation.

Functionalized MoS2-erlotinib produces hyperthermia under NIR

“…The characteristic peak of MoS 2 is between 260 to 300 nm. The characteristic peak of HA is around 192 nm, similar to literatures [54, 55]. The characteristic peak of Er is around 335 nm in accordance with references [56, 57].…”

Hyaluronic Acid: The Influence of Molecular Weight on Structural, Physical, Physico-Chemical, and Degradable Properties of Biopolymer

“…It is known that HA degrades when exposed to reactive oxygen species. The impact of various oxidizing agents such as ozone, UV light, hydrogen peroxide and others on HA was studied [ 119 , 120 , 121 , 122 , 123 , 124 , 125 ]. Unfortunately, there is no information available about the dependence of the rate of oxidative degradation of HA on its molecular weight as only one sample of HA was studied in most articles.…”

Gamma-Irradiation-Prepared Low Molecular Weight Hyaluronic Acid Promotes Skin Wound Healing

“…It is proposed that although HMWHA is used in various medical sciences, LMWHA may provide potential beneficial effects for wound healing. However, until now, the preparation of HA with a specific molecular weight has been a complex work that is not easy to control [21]. To fabricate LMWHA efficiently, several scholars used physical (ultrasound, ozone, electron beam, γ-irradiation and thermal treatment) and chemical methods (enzyme and acid degradation) to break the primary bond of HMWHA [21,22,23].…”