Hyaluronic Acid: The Influence of Molecular Weight on Structural, Physical, Physico-Chemical, and Degradable Properties of Biopolymer

Snetkov, Petr; Zakharova, Kseniia; Морозкина, С. Н.; Olekhnovich, Roman; Uspenskaya, M. V.

doi:10.3390/polym12081800

Cited by 269 publications

(231 citation statements)

References 133 publications

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“…Therefore, the pseudoplastic flow of the formulated hydrogel is confirmed from the obtained results. Our results are in agreement with the available information in the literature on the relationship between shear rate and viscosity [ 36 ].…”

Section: Resultssupporting

confidence: 92%

“…This special flow characteristic of hyaluronic acid hydrogel could be due to the breakdown of the structural configuration through the interruption of the existing intermolecular interactions between polymeric chains upon application of shear. There is further reoccurrence of polymerization between the molecules due to the force of the van der Waals interaction between the molecules upon removal of such shear [ 35 , 36 ]. The present study outcomes indicate that the optimal formulation of the HA-FS-NTF could facilitate the mean residence time within the oral cavity.…”

Section: Resultsmentioning

confidence: 99%

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Oral Gel Loaded by Fluconazole‒Sesame Oil Nanotransfersomes: Development, Optimization, and Assessment of Antifungal Activity

2020

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Candidiasis is one of the frequently encountered opportunistic infections in the oral cavity and can be found in acute and chronic presentations. The study aimed to develop fluconazole-loaded sesame oil containing nanotransfersomes (FS-NTF) by the thin-layer evaporation technique to improve the local treatment of oral candidiasis. Optimization of the formulation was performed using the Box‒Behnken statistical design to determine the variable parameters that influence the vesicle size, entrapment efficiency, zone of inhibition, and ulcer index. Finally, the formulated FS-NTF was embedded within the hyaluronic acid‒based hydrogel (HA-FS-NTF). The rheological behavior of the optimized HA-FS-NTF was assessed and the thixotropic behavior with the pseudoplastic flow was recorded; this is desirable for an oral application. An in vitro release study revealed the rapid release of fluconazole from the HA-FS-NTF. This was significantly higher when compared with the fluconazole suspension and hyaluronic acid hydrogel containing fluconazole. Correspondingly, the ex vivo permeation was also found to be higher in HA-FS-NTF in sheep buccal mucosa (400 μg/cm2) when compared with the fluconazole suspension (122 μg/cm2) and hyaluronic acid hydrogel (294 μg/cm2). The optimized formulation had an inhibition zone of 14.33 ± 0.76 mm and enhanced antifungal efficacy for the ulcer index (0.67 ± 0.29) in immunocompromised animals with Candida infection; these findings were superior to those of other tested formulations. Hence, it can be summarized that fluconazole can effectively be delivered for the treatment of oral candidiasis when it is entrapped in a nanotransfersome carrier and embedded into cross-linked hyaluronic acid hydrogel.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Oral Gel Loaded by Fluconazole‒Sesame Oil Nanotransfersomes: Development, Optimization, and Assessment of Antifungal Activity

2020

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“…The silk-HA scaffold could provide a more suitable microenvironment of biomimetic composition and structure for cell proliferation (Lu et al, 2011;Ding et al, 2017). The biological effects of HA depended heavily on molecular weight (Snetkov et al, 2020). The molecular weight of HA in human synovial fluid is very high, with the range of 2,000-10,000 kDa (Ghosh and Guidolin, 2002).…”

Section: Biocompatibility Of Silk-ha Scaffoldsmentioning

confidence: 99%

Fabrication of Silk-Hyaluronan Composite as a Potential Scaffold for Tissue Repair

Liu

et al. 2020

Front. Bioeng. Biotechnol.

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Interest is rapidly growing in the design and preparation of bioactive scaffolds, mimicking the biochemical composition and physical microstructure for tissue repair. In this study, a biomimetic biomaterial with nanofibrous architecture composed of silk fibroin and hyaluronic acid (HA) was prepared. Silk fibroin nanofiber was firstly assembled in water and then used as the nanostructural cue; after blending with hyaluronan (silk:HA = 10:1) and the process of freeze-drying, the resulting composite scaffolds exhibited a desirable 3D porous structure and specific nanofiber features. These scaffolds were very porous with the porosity up to 99%. The mean compressive modulus of silk-HA scaffolds with HA MW of 0.6, 1.6, and 2.6 × 106 Da was about 28.3, 30.2, and 29.8 kPa, respectively, all these values were much higher than that of pure silk scaffold (27.5 kPa). This scaffold showed good biocompatibility with bone marrow mesenchymal stem cells, and it enhanced the cellular proliferation significantly when compared with the plain silk fibroin. Collectively, the silk-hyaluronan composite scaffold with a nanofibrous structure and good biocompatibility was successfully prepared, which deserved further exploration as a biomimetic platform for mesenchymal stem cell-based therapy for tissue repair.

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“…When the molar mass of HA reaches 20–200 kDa, the macromolecules take part in biological processes such as embryonic development, wound healing, and ovulation. By contrast, high-molar-mass HA (>500 kDa) has anti-angiogenic activity, and can function as a space filler and a natural immunologic depressant [ 28 ]. The HA biopolymer provides a wide range of pharmacological activities, including anti-inflammatory, wound healing and tissue regenerating, immunomodulatory, anti-cancer, anti-proliferative, anti-diabetic, anti-aging, skin repairing, and cosmetic properties [ 27 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Versatile Use of Chitosan and Hyaluronan in Medicine

Valachová

Šoltés

2021

Molecules

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Chitosan is industrially acquired by the alkaline N-deacetylation of chitin. Chitin belongs to the β-N-acetyl-glucosamine polymers, providing structure, contrary to α-polymers, which supply food with energy. Another β-polymer providing structure is hyaluronan. A lot of studies have been performed on chitosan to explore its industrial use. Since chitosan is biodegradable, non-toxic, bacteriostatic, and fungistatic, it has numerous applications in medicine. Hyaluronan, one of the major structural components of the extracellular matrix in vertebrate tissues, is broadly exploited in medicine as well. This review summarizes the main areas where these two biopolymers have an impact. The reviewed areas mostly cover most medical applications, along with non-medical applications, such as cosmetics.

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Hyaluronic Acid: The Influence of Molecular Weight on Structural, Physical, Physico-Chemical, and Degradable Properties of Biopolymer

Cited by 269 publications

References 133 publications

Oral Gel Loaded by Fluconazole‒Sesame Oil Nanotransfersomes: Development, Optimization, and Assessment of Antifungal Activity

Oral Gel Loaded by Fluconazole‒Sesame Oil Nanotransfersomes: Development, Optimization, and Assessment of Antifungal Activity

Fabrication of Silk-Hyaluronan Composite as a Potential Scaffold for Tissue Repair

Versatile Use of Chitosan and Hyaluronan in Medicine

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