Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces

Muthusamy, Saraladevi; Kanagarajan, Selvaraju; Ponnusamy, Shanmugasundaram

doi:10.2225/vol11-issue3-fulltext-8

Cited by 81 publications

(63 citation statements)

References 22 publications

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“…Recently, these markers were successfully used Table 1 and Fig. 1 to calculate the intra or inter-specific genetic diversity in different domestic and wild species (Escandón et al 2005;Muthusamy et al 2008;Li et al 2011). Present study demonstrates the efficacy of RAPD and ISSR markers for the analysis of genetic diversity in Mucuna species and indicates that these markers could be successfully used to assess genetic variation among the accessions of Mucuna species.…”

Section: Discussionmentioning

confidence: 62%

Assessment of genetic diversity in Mucuna species of India using randomly amplified polymorphic DNA and inter simple sequence repeat markers

Patil

Pawar

Rane

et al. 2016

Physiol Mol Biol Plants

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Genus Mucuna which is native to China and Eastern India comprises of perennial climbing legume with long slender branches, trifoliate leaves and bear green or brown pod covered with soft or rigid hairs that cause intense irritation. The plants of this genus are agronomically and economically important and commercially cultivated in India, China and other regions of the world. The high degrees of taxonomical confusions exist in Mucuna species that make authentic identification and classification difficult. In the present study, the genetic diversity among the 59 accessions of six species and three varieties of M. pruriens has been assessed using DNA fingerprinting based molecular markers techniques namely randomly amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and combined dataset of RAPD and ISSR. Also, genetic relationship among two endemic species of Mucuna namely M. imbricata and M. macrocarpa and two varieties namely IIHR hybrid (MHR) and Dhanwantari (MD) with other species under study was investigated by using cluster analysis and principal coordinate analysis.The cluster analysis of RAPD, ISSR and combined dataset of RAPD and ISSR clearly demonstrated the existence of high interspecific variation than intra-specific variation in genus Mucuna. The utility and efficacy of RAPD and ISSR for the study of intra species and interspecies genetic diversity was evident from AMOVA and PCoA analysis. This study demonstrates the genetic diversity in Mucuna species and indicates that these markers could be successfully used to assess genetic variation among the accessions of Mucuna species.

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Section: Discussionmentioning

confidence: 62%

Assessment of genetic diversity in Mucuna species of India using randomly amplified polymorphic DNA and inter simple sequence repeat markers

Patil

Pawar

Rane

et al. 2016

Physiol Mol Biol Plants

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“…However, in rice bean (Vigna umbellata (Thunb.) Ohwi et H. Ohashi) more polymorphic loci were detected with RAPD (70.30%) than with ISSR fingerprinting (61.79%) (Muthusamy et al 2008).…”

Section: Genetic Classificationmentioning

confidence: 96%

Morphological and molecular diversity within Algerian cowpea (Vigna unguiculata (L.) Walp.) landraces

Ghalmi

Malice

Jacquemin

et al. 2009

Genet Resour Crop Evol

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Twenty landraces of cowpea (Vigna unguiculata (L.) Walp.) scattered throughout Algeria were compared through morphological and genetic characterization. At the morphological level, for qualitative characters there was no intra-landrace variation and for quantitative characters the variations were low except for landrace NAG2 Three different cultigroups were located in Algeria: Biflora that was dominant in the Sahara, Melanophtalmus in the North and Unguiculata including one landrace in Kabylia and two in Sahara. The AMOVA analysis indicated that the genetic variation was lower within than among agro-ecological regions. A Mantel test, revealed a correlation between the qualitative morphological data and the geographical data (R = 0.28; P \ 0.01), indicating that the degree of morphological change among landraces was roughly proportional to the geographical distances separating them.Genetic diversity was analyzed by using 11 random amplified polymorphic DNA (RAPD) and 12 intersimple sequence repeat (ISSR) markers. No intralandrace variability was found. The eleven RAPD primers yielded 77 bands, of which 45 (58.44%) were polymorphic; the genetic similarity ranged from 66.0 to 96.7%. The twelve ISSR primers provided a total of 104 bands, of which 65 (62.5%) were polymorphic; the genetic similarity ranged from 62.8 to 97.8%. cluster analysis showed a good match between genetic background and geographical distribution, which was confirmed by the results of the Mantel test. In particular, geographical data and genetic data were found to be correlated: (R = 0.33; P \ 0.01) for RAPD, (R = 0.37; P \ 0.01) for ISSR, and (R = 0.33; P \ 0.01) for a combined RAPD-ISSR dataset. Moreover, despite the absence of significant correlation between morphological and RAPD data (R = 0.14; P = 0.14), significant correlations between morphological data and both ISSR (R = 0.27, P \ 0.05) and a combined RAPD-ISSR dataset (R = 0.22, P \ 0.05) were noted. ISSR markers were better linked to morphological variation than were RAPD markers. However, despite this, genetic distances among these landraces were found to be essentially the same no matter which markers were used.

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“…This class of markers does not require any prior knowledge about the sequences to be amplified and shows high polymorphism in the material, being very useful in studies of genetic diversity, phylogeny, genomics and evolutionary biology (Reddy et al, 2002). The ISSR is being used by several authors in the molecular characterization of many plant species, such as: tomato (Kamel et al, 2010), rice bean (Muthusamy, et al, 2008) and coffee (Masumbuko & Bryngelsson, 2006). When compared with the SSR (simple sequence repeats) markers (Goulão & Oliveira, 2001), AFLP (amplified fragment length polymorphism) (Saini et al, 2004), RFLP (restriction fragment length polymorphism) (Kesari et al, 2010) and RAPD (randomly amplified polymorphic DNA) (Marotti et al, 2007) the ISSR markers were the most efficient to obtain a large number of polymorphic bands.…”

Section: Introductionmentioning

confidence: 99%

Variabilidade genética em tomate (Solanum lycopersicon Mill.) por marcadores ISSR

Aguilera

Pessoni

Rodrigues

et al. 2011

Agraria

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In this study we used ISSR molecular markers to evaluate the genetic variability of 96 tomato accessions from Banco de Germoplasma de Hortaliças (BGH) of Universidade Federal de Viçosa, Minas Gerais, Brazil. Ten ISSR primers were individually amplified to allow the differentiation of the material. All ten primers generated 144 DNA bands, 53 being polymorphic, with an average of 14.4 per primer. The profiles generated by primer 840-(GA) 8 YT contained the largest number of polymorphic bands (13 bands). The primer 855-(AC) 8 YT detected the greatest differentiation of the accessions (12 accessions) while the primer HBH-884(AG) 7 did not detect any. The evaluation of the dendrogram obtained by UPGMA and Tocher' grouping method allowed the differentiation of all the accessions. Accession BGH-980 was located in a separate group, being the most divergent amongst the accessions tested by both methods. DNA profiles based on ISSR markers revealed the potential of the digital fingerprints in the diagnosis of all the accessions. From the results obtained in this study, ISSR markers have a high efficiency to differentiate the germplasm of wild species, besides origin studies.

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Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces

Cited by 81 publications

References 22 publications

Assessment of genetic diversity in Mucuna species of India using randomly amplified polymorphic DNA and inter simple sequence repeat markers

Assessment of genetic diversity in Mucuna species of India using randomly amplified polymorphic DNA and inter simple sequence repeat markers

Morphological and molecular diversity within Algerian cowpea (Vigna unguiculata (L.) Walp.) landraces

Variabilidade genética em tomate (Solanum lycopersicon Mill.) por marcadores ISSR

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