Efficiency of Lead and Cadmium Excretion in Populations of Orchesella cincta (Collembola) from Various Contaminated Forest Soils

Straalen, Nico M. van; Burghouts, T. B. A.; Doornhof, M. J.; Groot, G. M.; Janssen, M. P. M.; Joosse, Els N. G.; Meerendonk, J. H. van; Theeuwen, J. P. J. J.; Verhoef, H.A.; Zoomer, H. R.

doi:10.2307/2403992

Cited by 135 publications

(54 citation statements)

References 36 publications

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“…Previously, selection was inferred for populations of O. cincta living in metal contaminated areas based on Cd excretion efficiency (EE), growth rate (RGR), age at maturity and maintainance of growth during Cd exposure (Van Straalen et al, 1987;Posthuma, 1990;Posthuma et al, 1992). Genetic variation was indeed determined for EE, RGR and another characteristic associated with Cd tolerance, moulting frequency (Posthuma et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…Origin and treatment of animals Approximately 500 O. cincta individuals were randomly collected from the reference site Roggebotzand (The Netherlands, Cd concentration in litter o0.005 mmol/g; Van Straalen et al, 1987). They were reared in a climate room (201C, 75% humidity, LD 12:12 h) in PVC jars on a moist plaster of Paris layer in which they could feed on algae present on twigs.…”

Section: Methodsmentioning

confidence: 99%

“…Field-selected tolerance to heavy metals has been reported for Orchesella cincta (Arthropoda: Collembola, springtails) populations occurring at metal-contaminated mining sites (Van Straalen et al, 1987). Populations from mining sites were characterized by a higher cadmium (Cd) excretion efficiency and a lower Cd-induced growth reduction (Posthuma, 1990;Posthuma et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Additive genetic variation of transcriptional regulation: metallothionein expression in the soil insect Orchesella cincta

et al. 2005

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Field-selected metal tolerance in Orchesella cincta is correlated with overexpression of the single copy cadmium (Cd) inducible metallothionein (mt). Previously, we have demonstrated large phenotypic variation in mt gene expression, and a higher frequency of high-expression phenotypes in a tolerant population. Here, we describe midparentoffspring regression analysis of mt gene expression in a laboratory culture originating from a noncontaminated natural population. Families were either not exposed (n ¼ 47) or exposed to 0.5 mmol Cd per gram dry food (n ¼ 46). Mean mt gene expressions normalized to 28S rRNA and b-actin RNA were generated using real-time RT-PCR applied to parents and offspring RNA and subjected to regression analysis. A significant heritability (h 2 ) for mt gene expression was estimated between 0.36 (b-actin normalized) and 0.46 (28S normalized) in Cd exposed families. Nontreated families did not yield a significant h 2 value. Restriction Fragment Length Polymorphism analysis of the metallothionein promoter sequence revealed eight promoter alleles that show structural variation. Three alleles show increased frequencies in families with high mt expression. Another gene, croquemort (isolated from a differential screening for 1 mmole Cd treatment) showed no h 2 of gene expression in response to 0.5 mmol Cd. This gene codes for a receptor-protein involved in recognition of apoptotic cells and may participate in the general stress response. The present data suggest that evolution of metal tolerance in O. cincta can occur in the field by selection for high mt expression due to structural changes in mt cis-regulation. Heredity (2006) 96, 85-92.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Additive genetic variation of transcriptional regulation: metallothionein expression in the soil insect Orchesella cincta

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“…This epithelium contains granules, probably filled with calcium phosphate, which may be excreted into the gut lumen. These granules probably play a role in the excretion of an overload of lead in the food [457,894].…”

Section: One-compartment Kineticsmentioning

confidence: 99%

Dynamic Energy and Mass Budgets in Biological Systems

Kooijman

2000

793

1,202

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second editionDynamic Energy Budget theory unifies the commonalities between organisms as prescribed by the implications of energetics, which link different levels of biological organization (cells, organisms and populations). The theory presents simple mechanistic rules that describe the uptake and use of energy and nutrients and the consequences for physiological organization throughout an organism's life cycle, including the relationships between energetics and aging and the effects of toxicants. In this new edition, the theory is broadened to encompass the fluxes of both energy and mass. All living organisms are now covered in a single quantitative framework, the predictions of which are tested against a wide variety of experimental results at the various levels of biological organization. The theory explains many general observations, such as the body size scaling relationships of certain physiological traits, and provides a theoretical basis for the widely used method of indirect calorimetry. In each case, the theory is developed in elementary mathematical terms, but a more detailed discussion of the methodological aspects of mathematical modelling is also included, making the book suitable for biologists and mathematicians with a broad interest in both fundamental and applied quantitative problems in biology.Bas Kooijman is Professor of Applied Theoretical Biology at the Vrije Universiteit in Amsterdam and is also head of the University's Department of Theoretical Biology. His research focuses on mathematical biology, in particular ecotoxicology which provided the framework for the original version of his Dynamic Energy Budget theory published in 1993 (Dynamic Energy Budgets in Biological Systems -Theory and Applications in Ecotoxicology). From the first edition:"This book is an excellent scientific product that informs and forces contemplation of issues relating to population and community ecology. The author has made a complex subject coherent." Thomas G. Hallam, Bulletin of Mathematical Biology "... a very useful and general tool to select more ecologically sound process equations and parameters in ecological models." Sven Erik Jørgensen, Ecological Modelling "The author has made a significant contribution to the problem of modelling the dynamics of biological populations at the level of the individual by synthesizing this very complicated subject into a relatively short list of general assumptions and putting the energetics of sub-models for vital rates on a solid basis." Jim Cushing, Mathematical Biosciences "The family of idealized models offered in this book is capable of playing a role analogous to that of Lotka-Volterra models in population dynamics ... I am confident that any model(s) capable of occupying this niche will be strongly influenced by the ideas in this book." Roger Nisbet, Ecology 9 LIVING TOGETHER Interaction between organisms: The spectrum from competition to preypredator systems. Evaluation of the consequences of the DEB model for population dynamics, food chains, and communities....

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“…The Cd-induced transcriptional profile includes genes related to metal trafficking (metallothionein, phytochelatin synthase, ion pumps), antioxidant defense (superoxide dismutase, catalase, peroxidases), sulphur salvage (sulphate uptake proteins, synthesis of methionine, cysteine and glutathione), iron metabolism (ferrotransferrin, iron transporters, ferritine), and the innate immune response (serine proteases and antimicrobial peptides). For an overview of the metal-induced stress response see Van Straalen and Roelofs (2006).…”

Section: Mechanisms Of the Cadmium-induced Stress Responsementioning

confidence: 99%

Molecular mechanisms of heavy metal tolerance and evolution in invertebrates

Janssens¹,

Roelofs²,

Straalen³

2009

Insect Science

144

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Following the genomics revolution, our knowledge of the molecular mechanisms underlying defenses against stress has been greatly expanded. Under strong selective pressure many animals may evolve an enhanced stress tolerance. This can be achieved by altering the structure of proteins (through mutations in the coding regions of genes) or by altering the amount of protein (through changes in transcriptional regulation). The latter type of evolution can be achieved by substitutions in the promoter of the gene of interest (cisregulatory change) or by altering the structure or amount of transcriptional regulator proteins (trans-regulatory change). The metallothionein system is one of the best studied stress response systems in the context of heavy metals. Metallothionein expression is assumed to be regulated by metal transcription factor 1 (MTF-1); however, up to now the involvement of MTF-1 has only been proven for some vertebrates and Drosophila. Data on invertebrates such as nematodes and earthworms suggest that other mechanisms of metallothionein induction may be present. A detailed study of Cd tolerance was done for a species of soilliving springtail, Orchesella cincta. The metallothionein gene of this species is overexpressed in metal-exposed field populations. Analysis of the metallothionein promoter has demonstrated extensive polymorphisms that have a functional significance, as shown in bioreporter assays. In a study comparing 20 different populations, the frequency of a high-expresser promoter allele was positively correlated with the concentration of metals in soil, especially Cd. The springtail study shows that cis-regulatory change of genes involved in the cellular stress response may contribute to evolution of metal tolerance.

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Efficiency of Lead and Cadmium Excretion in Populations of Orchesella cincta (Collembola) from Various Contaminated Forest Soils

Cited by 135 publications

References 36 publications

Additive genetic variation of transcriptional regulation: metallothionein expression in the soil insect Orchesella cincta

Additive genetic variation of transcriptional regulation: metallothionein expression in the soil insect Orchesella cincta

Dynamic Energy and Mass Budgets in Biological Systems

Molecular mechanisms of heavy metal tolerance and evolution in invertebrates

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