Efficiency of antibiotics and gamma irradiation in eliminating Streptomyces strains isolated from paintings of ancient Egyptian tombs

Abdel-Haliem, Mahmoud E.F.; Ali, Mona F.; Ghaly, Mohamed F.; Sakr, Akmal A.

doi:10.1016/j.culher.2012.03.009

Cited by 14 publications

(5 citation statements)

References 25 publications

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“…According to Abdel-Haliem et al . [ 55 ], the physical and chemical characteristics of Egyptian soil, as well as the soil microbial taxa, differed depending on the location and environment. El-Bahariya Oasis is a significant location in the western desert of Egypt.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Potentiality of Actinomycete-Derived Extract with Anti-Biofilm, Antioxidant, and Cytotoxic Capabilities as a Natural Combating Strategy for Multidrug-Resistant ESKAPE Pathogens

Elsayed

Alshammari

Sharaf

2022

J. Microbiol. Biotechnol.

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The global increase in multidrug-resistant (MDR) bacteria has inspired researchers to develop new strategies to overcome this problem. In this study, 23 morphologically different, soil-isolated actinomycete cultures were screened for their antibacterial ability against MDR isolates of ESKAPE pathogens. Among them, isolate BOGE18 exhibited a broad antibacterial spectrum, so it was selected and identified based on cultural, morphological, physiological, and biochemical characteristics. Chemotaxonomic analysis was also performed together with nucleotide sequencing of the 16S rRNA gene, which showed this strain to have identity with Streptomyces lienomycini . The ethyl acetate extract of the cell-free filtrate (CFF) of strain BOGE18 was evaluated for its antibacterial spectrum, and the minimum inhibitory concentration (MIC) ranged from 62.5 to 250 μg/ml. The recorded results from the in vitro anti-biofilm microtiter assay and confocal laser scanning microscopy (CLSM) of sub-MIC concentrations revealed a significant reduction in biofilm formation in a concentration-dependent manner. The extract also displayed significant scavenging activity, reaching 91.61 ± 4.1% and 85.06 ± 3.14% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. A promising cytotoxic ability against breast (MCF-7) and hepatocellular (HePG2) cancer cell lines was obtained from the extract with IC 50 values of 47.15 ± 13.10 and 122.69 ± 9.12 μg/ml, respectively. Moreover, based on gas chromatography-mass spectrometry (GC-MS) analysis, nine known compounds were detected in the BOGE18 extract, suggesting their contribution to the multitude of biological activities recorded in this study. Overall, Streptomyces lienomycini BOGE18-derived extract is a good candidate for use in a natural combating strategy to prevent bacterial infection, especially by MDR pathogens.

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Section: Discussionmentioning

confidence: 99%

Antagonistic Potentiality of Actinomycete-Derived Extract with Anti-Biofilm, Antioxidant, and Cytotoxic Capabilities as a Natural Combating Strategy for Multidrug-Resistant ESKAPE Pathogens

Elsayed

Alshammari

Sharaf

2022

J. Microbiol. Biotechnol.

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“…In addition, the chemical and physical characteristics of soil, as well as the soil microbial taxa, differed depending on the environmental condition. Microbials must adapt and develop to withstand distinct stress circumstances and, as a result, have the potential to produce novel active compounds with speci c bioactivities [23]. Therefore, our three potential actinomycetes from Muna island soil represent an unexplored source to unravel potential active compounds.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and antibiofilm activities from soil Streptomyces spp. isolated from Muna Island, Indonesia against multidrug-resistant clinical isolates.

Prastya,

Simbolon,

Priyanto

et al. 2024

Preprint

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The global increase in multidrug-resistant (MDR) bacterial infection has rapidly been gaining concern and leading for investigating new strategies to tackle this problem. In this study, the antibacterial potential of 25 soil actinomycetes strain has been evaluated by initial screening against MDR bacterial strains including Escherichia coli strain M19, Pseudomonas aeruginosa strain M19, Klebsiella pneumoniae strain M19, Bacillus subtilis strain M19, and Methicillin Resistant Staphylococcus aureus (MRSA). Among them, three actinomycetes isolates encoded APM-7, APM-11, and APM-21 exhibited strong and a broad antibacterial spectrum, hence there were selected for further study to extract its secondary metabolites following antibacterial, and antibiofilm assessment. The ethyl acetate extract of those three selected actinomycetes were evaluated for its antibacterial spectrum, and the minimum inhibitory concentration (MIC) ranged from 78 to 10000 µg/mL. Those extracts also displayed significant biofilm inhibition values ranging from 6.06 to 72.4%. Based on the results, APM-21 extract showed the best antibacterial and antibiofilm activities with the strongest values. Further, nucleotide sequencing of the 16S rRNA gene showed that these three potential strains APM-7, APM-11, and APM-21 to have identity with Streptomyces cyaneus, Streptomyces coerulescens, and Streptomyces panayensis, respectively. Moreover, based on Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis, two antibacterial compounds namely rancimanycin III, and enteromycin were detected in all those three extracts. Interestingly, APM-21 extract also contains two prominent antibacterial substances including paramagnetoquinone C, and caerulomycin I, suggesting their contribution to the most potential activities recorded in this study. Ultimately, our study provides new insights into a promising candidate for use in an active compound combating strategy to prevent MDR bacterial strains infection.

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“…Negligible spectral and chromatic alterations were observed after treatment by Fourier transform infrared (FTIR) and FT-Raman spectroscopy and CIELAB color data, respectively. Strains of Streptomyces isolated from ancient Egyptian paintings were killed using gamma irradiation without causing discoloration to the paintings or affecting the binding media mechanical properties [ 61 ]. Similarly, no negative effects of gamma radiation were observed on the texture and color of parchment for the studied doses able to combat biodeterioration phenomena [ 62 ].…”

Section: Physical Methodsmentioning

confidence: 99%

The Control of Cultural Heritage Microbial Deterioration

2020

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The microbial deterioration of cultural heritage includes physical and chemical damage as well as aesthetic alteration. With the technological advancement, a plethora of techniques for removing unwanted microorganisms have opened up new opportunities for microbiologists and conservators. This article reviews the most applied, up-to-date, and sustainable techniques developed for the control of cultural heritage microbial deterioration presenting noteworthy case studies. These techniques include chemical methods, i.e., traditional biocides and nanoparticles; physical methods, such as mechanical removal, UV irradiation, gamma radiation, laser cleaning, heat shocking, microwaves, and dry ice treatment; and biological methods, such as natural molecules with biocidal activity, enzymes, and microorganisms. The application of control systems requires the comprehension of their behavior toward the unwanted microorganisms and possible interactions with the heritage materials. This overview shows also the control methods drawbacks for the purpose of creating awareness in selecting the most suitable technique or combination of techniques.

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Efficiency of antibiotics and gamma irradiation in eliminating Streptomyces strains isolated from paintings of ancient Egyptian tombs

Cited by 14 publications

References 25 publications

Antagonistic Potentiality of Actinomycete-Derived Extract with Anti-Biofilm, Antioxidant, and Cytotoxic Capabilities as a Natural Combating Strategy for Multidrug-Resistant ESKAPE Pathogens

Antagonistic Potentiality of Actinomycete-Derived Extract with Anti-Biofilm, Antioxidant, and Cytotoxic Capabilities as a Natural Combating Strategy for Multidrug-Resistant ESKAPE Pathogens

Antibacterial and antibiofilm activities from soil Streptomyces spp. isolated from Muna Island, Indonesia against multidrug-resistant clinical isolates.

The Control of Cultural Heritage Microbial Deterioration

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