Efficiency of a Lactobacillus plantarum-xylanase combination on growth performances, microflora populations, and nutrient digestibilities of broilers infected with Salmonella Typhimurium

Abstract

Three experiments were performed to assess the ability of a Lactobacillus plantarum probiotic combined with a xylanase to reduce the effects of Salmonella Typhimurium infection in broiler chickens from 1 to 30 or 42 d of age. Chicks were challenged at 3 d of age with 10(8) or 10(5) cfu Salmonella Typhimurium/chick. Four diets were studied: a wheat-based diet (C+) supplemented with 0.1 g/kg of xylanase (E) or 10(6) cfu/g of L. plantarum (P), or both (PE). Uninfected chicks fed the C diet were used as negative control (C-). Six or 8 chicks were housed per cage with 9 cages/treatment. Growth performance and feed conversion ratio (FCR) were recorded weekly. In experiment 1, bacterial enumeration in ceca was achieved using the fluorescent in situ hybridization technique. Salmonella enumeration was realized in excreta by microbiological cultures (experiments 2 and 3). Nutrient digestibilities and AME(n) were determined in experiment 3 from d 35 to 39. Infection with Salmonella Typhimurium led to a significant decrease in the daily weight gain (DWG) by 23.6 to 32.8%, whereas FCR was increased by 1.0 to 19.7%. Chickens fed the PE diet showed significantly improved performance in comparison with C+ birds (DWG: +12.5% in experiment 1; FCR: -2.1 to 8.6%), and in comparison with the P and E treatments (DWG: +6.3 to 8.3% in experiment 1; FCR: -2.7 to 6.4%). In experiment 3, the FCR was significantly improved by 3% with the PE diet in comparison with C- chickens. The PE combination tended to restore a microflora similar to that of uninfected broilers, whereas the P and E diets had less of an effect on the profile of bacterial communities. At slaughter age, Salmonella contamination was reduced by 2.00 and 1.85 log colony-forming units for the E and PE treatment, respectively. The PE diet significantly reduced the crude fat digestibility by 9.2%, in comparison with the C+ chickens. These results suggest that the combination between L. plantarum and a xylanase as feed additive could be effective for reduction of the detrimental effect after Salmonella Typhimurium infection of broilers.

Effect of zinc on growth performance, gut morphometry, and cecal microbial community in broilers challenged with Salmonella enterica serovar typhimurium

“…Infection with Salmonellas leads to a significant negative effect on growth performance of poultry (Vandeplas et al, 2009;Marcq et al, 2011;Wang et al, 2012), which was confirmed in this study. In the present study, there was a significant reduction in BWG after S. typhimurium challenge.…”

In Vitro Antagonistic Activity Evaluation of Lactic Acid Bacteria (LAB) Combined with Cellulase Enzyme Against Campylobacter jejuni Growth in Co-Culture

“…The LAB survival in the chicken excreta and their antagonist activity potential could be enhanced by glycanase enzyme, as stated by Vandeplas et al [38]. These authors showed that a combination between an enzyme of xylanase type and a Lactobacillus strain used as feed additive was able to reduce the Salmonella Typhimurium concentration in caeca of challenged chickens.…”

Effect of Dietary <i>Bacillus Subtilis </i>C14 and RX7 Strains on Growth Performance, Blood Parameter, and Intestinal Microbiota in Broiler Chickens Challenged with <i>Salmonella Gallinarum</i>

“…The decrease in weight gain might be mainly due to decreased feed intake. Our results was in consistent with previous reports on reduced weight gain and feed intake in poultry challenged with S. gallinarum (Gupta et al, 2005;Vandeplas et al, 2009;Marcq et al, 2011). Our results also demonstrated that the addition of B. subtilis into diets could enhance the growth performance of broilers challenged with S. gallinarum.…”