Efficiency comparison of apigenin-7-O-glucoside and trolox in antioxidative stress and anti-inflammatory properties

Abstract

Objectives Chamomile has long been used as a medicinal plant due to its antioxidative and anti-inflammatory activity. Apigenin-7-O-glucoside (AG) is one of the major ethanol extract components from chamomile; however, the underlying mechanism remains unclear. Methods In this study, the antioxidant potential and the anti-inflammatory activities of AG were analysed and compared with those of trolox. We demonstrate the protective effects of AG on free radical-induced oxidative damage of DNA, proteins and erythrocytes. Flow cytometry assay was used to detect ROS production. Additionally, the expression of anti-oxidation-related and inflammation-related factors was detected by ELISA and Western blotting, respectively. Key findings AG and trolox showed different efficiency as antioxidant in different experimental systems. AG had similar effect as trolox to inhibit H 2 O 2-induced ROS production in RAW264.7 cells, while exerted stronger inhibition against free radical-induced oxidative damage on erythrocytes than trolox. Interestingly, compared with trolox, AG also had stronger inhibitory effect on LPS-induced NF-κB/NLRP3/caspase-1 signalling in RAW246.7 cells. Conclusions These results suggest the potential of AG as a pharmaceutical drug for anti-oxidation and anti-inflammation, and the combined usage of AG and trolox might promote its efficacy. Our findings will provide new insights into the development of new drugs with antioxidative and anti-inflammatory functions.

Bioactive Apigenin loaded oral nano bilosomes: Formulation optimization to preclinical assessment

“…It is found in various natural sources like vegetables and fruits ( Funakoshi-Tago et al, 2011 ). There are number of pharmacological activities like antidiabetic ( Choi, et al, 2014 ), anticancer ( Lu et al, 2020 ), anti-oxidant ( Darabi et al, 2020 ) and anti-inflammatory activity ( Wang et al, 2020 ) have been reported. It has poor aqueous solubility and belongs to BCS-II class category ( Huang et al, 2016 ).…”

Chamomile as a potential remedy for obesity and metabolic syndrome

“…The anti-inflammatory effects of chamomile preparations or their isolated compounds have been identified in studies of multiple experimental models (Miguel et al, 2015[ 90 ]; Wang et al, 2020[ 138 ]; Cavalcante et al, 2020[ 17 ]) and when used to treat various diseases associated with the development and progression of inflammatory processes (Jabri et al, 2020[ 54 ]; Wang et al, 2020[ 138 ]). For example, treatment of macrophages with lipopolysaccharide (LPS) from Escherichia coli is a well-established experimental system for studies of inflammation and stimulates a range of signaling pathways, including NF-κB-mediated signaling that enhances the transcription of mRNA encoding pro-inflammatory cytokines such as IL-1β, IL-6, TNF-α (Tanaka et al, 2014[ 130 ]).…”

“…The incubation of macrophages with LPS in the presence of polyphenol-rich chamomile extracts or apigenin-7-O-glucoside decreased production of pro-inflammatory cytokines IL-1β, IL-6 and TNF-α (Drummond et al, 2013[ 27 ]; Miguel et al, 2015[ 90 ]). Apigenin-7-O-glucoside also had strong inhibitory effects on LPS-induced NF-κB/NLRP3/caspase-1 signaling in macrophage RAW246.7 cells (Wang et al, 2020[ 138 ]). Moreover, apigenin-7-O-glucoside was found to suppress LPS-induced mRNA expression of iNOS and cyclooxygenase-2 (COX-2), lowering release of inflammatory mediators, • NO and prostaglandin E2 (PGE2) (Hu et al, 2016[ 53 ]).…”

“…(4) Anti-inflammatory action . Chamomile preparations are connected with the inhibition of synthesis of inflammatory mediators, • NO and prostaglandins, and pro-inflammatory cytokines and chemokines (Kim et al, 2011[ 62 ]; Hu et al, 2016[ 53 ]; Ma et al, 2020[ 80 ]; Wang et al, 2020[ 138 ]). Obesity is characterized by development of chronic inflammation with NF-κB being the major contributor.…”

Potential Mechanisms of Plant-Derived Natural Products in the Treatment of Cervical Cancer

“…It has a strong inhibitory effect on tumor-cell viability in vivo and in vitro. Its derivative apigenin 7-glucoside has anti-inflammatory, antioxidant and anticancer activities [32]. Apigenin 7-glucoside significantly suppressed the proliferation of HeLa cells, and the IC 50 value at 48 h was 47.26 µmol/L.…”