Efficiency and specificity of CTXϕ chromosomal integration:
            <i>dif</i>
            makes all the difference

Boyd, E. Fidelma

doi:10.1073/pnas.1000310107

Cited by 8 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cholera toxin is another example of phage-related toxin. Virulent strains of Vibrio cholerae are known to carry a filamentous bacteriophage CTXϕ [178]. This ssDNA phage is a member of the Inoviridae family, and it is shown to carry the gene of cholera toxin as well as two others [179].…”

Section: Phage-related Bacterial Toxinsmentioning

confidence: 99%

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Podlacha

Grabowski

Kosznik-Kawśnicka

et al. 2021

IJMS

View full text Add to dashboard Cite

Bacteriophages are viruses infecting bacterial cells. Since there is a lack of specific receptors for bacteriophages on eukaryotic cells, these viruses were for a long time considered to be neutral to animals and humans. However, studies of recent years provided clear evidence that bacteriophages can interact with eukaryotic cells, significantly influencing the functions of tissues, organs, and systems of mammals, including humans. In this review article, we summarize and discuss recent discoveries in the field of interactions of phages with animal and human organisms. Possibilities of penetration of bacteriophages into eukaryotic cells, tissues, and organs are discussed, and evidence of the effects of phages on functions of the immune system, respiratory system, central nervous system, gastrointestinal system, urinary tract, and reproductive system are presented and discussed. Modulations of cancer cells by bacteriophages are indicated. Direct and indirect effects of virulent and temperate phages are discussed. We conclude that interactions of bacteriophages with animal and human organisms are robust, and they must be taken under consideration when using these viruses in medicine, especially in phage therapy, and in biotechnological applications.

show abstract

Section: Phage-related Bacterial Toxinsmentioning

confidence: 99%

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Podlacha

Grabowski

Kosznik-Kawśnicka

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…XerC and XerD are tyrosine recombinases ( 28 ) that facilitate chromosomal integration of phages or other exogenous gene components ( 29 ). The presence of multiple copies of XerCD recombinase genes, dif sequences, and exogenous gene components around the dif sequences in the genomes in genetic lineage C might imply extensive integration of phages and associated gene components into bacterial chromosomes via site-specific recombination by the action of XerCD/ dif ( 30 ). A recent study showed that XerD could unload bacterial chromosomal complexes at the replication end, and such a mechanism is important in maintaining genome organization in all living organisms ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics Revealed Wide Intra-Species Genetic Heterogeneity and Lineage-Specific Genes of Akkermansia muciniphila

Sun

Jing

et al. 2022

Microbiol Spectr

View full text Add to dashboard Cite

Akkermansia muciniphila has been widely accepted as part of the next generation of probiotics. However, most current studies on A. muciniphila have focused on the application of type strain BAA835 T in the treatment of diseases, while few studies have reported on the genomic specificity, population structure, and functional characteristics of A. muciniphila species.

show abstract

“…Once CTXΦ recognizes TcpA pili on the surface, it injects it's (+)ssDNA genome in to the host cytoplasm of the host cell(s) (Karaolis et al, 1999 ). The (+)ssDNA genome of CTXΦ either converted into replicative double stranded pCTXΦ or exploits the two tyrosine recombinases, XerC and XerD, for irreversible integration into chromosomal DNA (Boyd, 2010 ). XerC and XerD recombinases are conserved among the bacteria chromosomes and their native function is to resolve the chromosome dimers.…”

Section: Vpi-1 and Cholera Pathogenesismentioning

confidence: 99%

Vibrio Pathogenicity Island-1: The Master Determinant of Cholera Pathogenesis

Kumar

Das

Kumar

2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Cholera is an acute secretory diarrhoeal disease caused by the bacterium Vibrio cholerae . The key determinants of cholera pathogenicity, cholera toxin (CT), and toxin co-regulated pilus (TCP) are part of the genome of two horizontally acquired Mobile Genetic Elements (MGEs), CTXΦ, and Vibrio pathogenicity island 1 (VPI-1), respectively. Besides, V. cholerae genome harbors several others MGEs that provide antimicrobial resistance, metabolic functions, and other fitness traits. VPI-1, one of the most well characterized genomic island (GI), deserved a special attention, because (i) it encodes many of the virulence factors that facilitate development of cholera (ii) it is essential for the acquisition of CTXΦ and production of CT, and (iii) it is crucial for colonization of V. cholerae in the host intestine. Nevertheless, VPI-1 is ubiquitously present in all the epidemic V. cholerae strains. Therefore, to understand the role of MGEs in the evolution of cholera pathogen from a natural aquatic habitat, it is important to understand the VPI-1 encoded functions, their acquisition and possible mode of dissemination. In this review, we have therefore discussed our present understanding of the different functions of VPI-1 those are associated with virulence, important for toxin production and essential for the disease development.

show abstract

Efficiency and specificity of CTXϕ chromosomal integration: dif makes all the difference

Cited by 8 publications

References 21 publications

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Interactions of Bacteriophages with Animal and Human Organisms—Safety Issues in the Light of Phage Therapy

Comparative Genomics Revealed Wide Intra-Species Genetic Heterogeneity and Lineage-Specific Genes of Akkermansia muciniphila

Vibrio Pathogenicity Island-1: The Master Determinant of Cholera Pathogenesis

Contact Info

Product

Resources

About