Vibrio Pathogenicity Island-1: The Master Determinant of Cholera Pathogenesis

Kumar, Ashok; Das, Bhabatosh; Kumar, Niraj

doi:10.3389/fcimb.2020.561296

Cited by 30 publications

(25 citation statements)

References 88 publications

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“…Comparison of the pathogenicity of T. asahii strains using a silkworm infection model. Highly pathogenic strains are useful for understanding the molecular mechanisms of pathogens because several pathogenic strains obtain virulence genes by horizontal gene transfer and gene mutation [35][36][37][38][39] . First, we identi ed T. asahii strains that are highly pathogenic to silkworms.…”

Section: Resultsmentioning

confidence: 99%

Development of an Efficient Gene-targeting System for Elucidating Infection Mechanisms of the Fungal Pathogen Trichosporon Asahii

Matsumoto

Nagamachi

Yoshikawa

et al. 2021

Preprint

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Trichosporon asahii is a pathogenic fungus that causes severe, deep-seated fungal infections in neutropenic patients. Elucidating the infection mechanisms of T. asahii based on genetic studies requires a specific gene-targeting system. Here, we established an efficient gene-targeting system in a highly pathogenic T. asahii strain identified using the silkworm infection model. By comparing the pathogenicity of T. asahii clinical isolates in a silkworm infection model, T. asahii MPU129 was identified as a highly pathogenic strain. Using an Agrobacterium tumefaciens-mediated gene transfer system, we obtained a T. asahii MPU129 mutant lacking the ku70 gene, which encodes the Ku70 protein involved in the non-homologous end-joining repair of DNA double-strand breaks. The ku70 gene-deficient mutant showed higher gene-targeting efficiency than the wild-type strain for constructing a mutant lacking the cnb1 gene, which encodes the beta-subunit of calcineurin. The cnb1 gene-deficient mutant showed reduced pathogenicity against silkworms compared with the parental strain. These results suggest that an efficient gene-targeting system in a highly pathogenic T. asahii strain is a useful tool for elucidating the molecular mechanisms of T. asahii infection.

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Section: Resultsmentioning

confidence: 99%

Development of an Efficient Gene-targeting System for Elucidating Infection Mechanisms of the Fungal Pathogen Trichosporon Asahii

Matsumoto

Nagamachi

Yoshikawa

et al. 2021

Preprint

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“…The pandemic virulence potential of V. cholerae resides in its MGE, harbouring both the CT and TCP apparel on the integrated CTXφ phage and Vibrio Pathogenic Island 1 (VPI-1), respectively [125]. TCP is essential for effective colonization of the intestinal epithelium in pandemic O1/O139 strains [126].…”

Section: The Toxin Coregulated Pilusmentioning

confidence: 99%

“…The VPI-1 harbours the receptor for the CTXφ phage, the major pilin of TCP (TcpA), allowing its entry into V. cholerae. It also regulates the CT production with ToxT, which also regulates TCP expression [48,125]. It is believed that acquisition of both these MGE is enough to convert environmental strains into pathogenic strains [125].…”

Section: The Toxin Coregulated Pilusmentioning

confidence: 99%

“…It also regulates the CT production with ToxT, which also regulates TCP expression [48,125]. It is believed that acquisition of both these MGE is enough to convert environmental strains into pathogenic strains [125]. Considering this information, it is clear that the gene acquisition by horizontal transfer is important for the toxigenic potential of V. cholerae.…”

Section: The Toxin Coregulated Pilusmentioning

confidence: 99%

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The Secretome of Vibrio cholerae

Mathieu-Denoncourt¹,

Giacomucci²,

Duperthuy³

2021

Infections and Sepsis Development

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Vibrio cholerae is a facultative human pathogen responsible for the cholera disease which infects millions of people worldwide each year. V. cholerae is a natural inhabitant of aquatic environments and the infection usually occurs after ingestion of contaminated water or food. The virulence factors of V. cholerae have been extensively studied in the last decades and include the cholera toxin and the coregulated pilus. Most of the virulence factors of V. cholerae belong to the secretome, which corresponds to all the molecules secreted in the extracellular environment such as proteins, exopolysaccharides, extracellular DNA or membrane vesicles. In this chapter, we review the current knowledge of the secretome of V. cholerae and its role in virulence, colonization and resistance. In the first section, we focus on the proteins secreted through conventional secretion systems. The second and third sections emphasize on the membrane vesicles and on the secretome associated with biofilms.

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“…Therefore, Vibriosis has caused significant impacts on human health and the development of aquaculture [68][69][70]. The pathogenicity of Vibrio is determined by multiple virulence factors encoded by its virulence genes [71][72][73][74]. Vibrio infects and destroys the host through a series of processes, including adhesion, invasion, immune escape, in vivo proliferation and production of toxins [46].…”

Section: Pathogenicity Of Vibriomentioning

confidence: 99%

Community Change and Pathogenicity ofVibrio

Huang¹,

Gao²,

Zhang³

et al. 2021

Infections and Sepsis Development

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Vibrio is a rod-shaped Gram-negative bacteria, which is widely distributed in marine and estuarine environments worldwide. It is an important component of the aquatic ecosystem and plays an important role in biogeochemical cycle. Its population dynamics are usually affected by climate and seasonal factors. Most of the Vibrios in the environment are not pathogenic, but some of them are pathogenic bacteria for human and animal, such as Vibrio cholerae, Vibrio vulnificus, Vibrio parahaemolyticus, and Vibrio anguillarum, etc., which are generally reported to be related to aquatic animal diseases and human food-borne diseases. Over the last couple of years, due to the influence of the rising seawater temperature and climate change, the incidence of diseases caused by Vibrio infection has increased significantly, which poses a great threat to human health and aquaculture. The research on pathogenic Vibrio has attracted more and more attention. The abundance and community changes of Vibrio in the environment are usually controlled by many biological and abiotic factors. The Vibrio pathogenicity is related to the virulence factors encoded by virulence genes. The process of Vibrio infecting the host and causing host disease is determined by multiple virulence factors acting together, instead of being determined by a single virulence factor. In this chapter, community changes of Vibrio, as well as the virulence factors of Vibrio and the related virulence genes of Vibiro are summarized, and their important roles in Vibrio infection are also discussed.

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Vibrio Pathogenicity Island-1: The Master Determinant of Cholera Pathogenesis

Cited by 30 publications

References 88 publications

Development of an Efficient Gene-targeting System for Elucidating Infection Mechanisms of the Fungal Pathogen Trichosporon Asahii

Development of an Efficient Gene-targeting System for Elucidating Infection Mechanisms of the Fungal Pathogen Trichosporon Asahii

The Secretome of Vibrio cholerae

Community Change and Pathogenicity ofVibrio

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