One of the approaches for understanding the functions of the genes predicted in the rice genome requires use of mutants. Mutations induced in a given genetic background provide opportunities to assign function to a given gene with a minimum of background genetic noise. This paper describes generation and initial characterization of a large set of Ethyl Methane Sulphonate (EMS) induced mutants in the upland rice variety Nagina22, through a National Initiative involving six Research Institutes namely National Research Centre on Plant Biotechnology, New Delhi; Indian Agricultural Research Institute, New Delhi; Tamil Nadu Agricultural University, Coimbatore; Directorate of Rice Research, Hyderabad; University of Agricultural Sciences, Bangalore and Punjab Agricultural University, Ludhiana, funded by the Department of Biotechnology (DBT), Government of India. The uniqueness of this collaborative effort is phenotyping for a range of traits that has led to identification of mutants for plant growth and architecture, flowering, maturity, grain number, shape and size, yield, phosphorus use efficiency, resistance to blast and bacterial leaf blight diseases, and tolerance to drought, salinity and herbicide. A set of 22, 292 mutagenised lines generated under this initiative and phenotyped for the traits enlisted above has resulted in the isolation of a few promising mutants which are being characterized. Shortly, these mutants will be registered and made available to the researchers in the country for use in studies in rice genetics, breeding and functional genomics. The mutant stock is expected to serve as a national resource for understanding rice biology as well as for use in genetic improvement of the crop.