EMS Induced Mutants of Upland Rice Variety Nagina22: Generation and Characterization

Abstract

One of the approaches for understanding the functions of the genes predicted in the rice genome requires use of mutants. Mutations induced in a given genetic background provide opportunities to assign function to a given gene with a minimum of background genetic noise. This paper describes generation and initial characterization of a large set of Ethyl Methane Sulphonate (EMS) induced mutants in the upland rice variety Nagina22, through a National Initiative involving six Research Institutes namely National Research Centre on Plant Biotechnology, New Delhi; Indian Agricultural Research Institute, New Delhi; Tamil Nadu Agricultural University, Coimbatore; Directorate of Rice Research, Hyderabad; University of Agricultural Sciences, Bangalore and Punjab Agricultural University, Ludhiana, funded by the Department of Biotechnology (DBT), Government of India. The uniqueness of this collaborative effort is phenotyping for a range of traits that has led to identification of mutants for plant growth and architecture, flowering, maturity, grain number, shape and size, yield, phosphorus use efficiency, resistance to blast and bacterial leaf blight diseases, and tolerance to drought, salinity and herbicide. A set of 22, 292 mutagenised lines generated under this initiative and phenotyped for the traits enlisted above has resulted in the isolation of a few promising mutants which are being characterized. Shortly, these mutants will be registered and made available to the researchers in the country for use in studies in rice genetics, breeding and functional genomics. The mutant stock is expected to serve as a national resource for understanding rice biology as well as for use in genetic improvement of the crop.

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

“…Use of chemically induced mutants such as ethyl methane sulfonic acid (EMS) has helped to create useful genetic variations in the past (Bhat et al, 2007;Till et al, 2007;Henry et al, 2014;Mohapatra et al, 2014). It has been shown that mainly GC to AT transitions were induced by EMS in Arabidopsis, maize and wheat, (Till et al, 2003;Till et al, 2004;Slade, 2005).…”

Comparing Hydroponics, Sand, and Soil Medium To Evaluate Contrasting Rice Nagina 22 Mutants for Tolerance to Phosphorus Deficiency

“…Nagina 22 is a well-known aus variety used routinely as a check for heat tolerance in rice (Jagadish et al, 2010). In a network project, EMS-induced stabilized N22 mutants were evaluated for different traits, such as tolerance to herbicide, heat, low P, water deficiency, and salinity and resistance to bacterial leaf blight and blast (Kulkarni et al, 2013(Kulkarni et al, , 2014Lima et al, 2015;Mithra et al, 2016;Mohapatra et al, 2014;Panigrahy et al, 2009Panigrahy et al, , 2011Panigrahy et al, , 2014Poli et al, 2013;Talla et al, 2016). As a part of the network project on functional genomics, a total of 2500 N22 mutants were screened at the Indian Institute of Rice Research (IIRR) under field conditions maintained with low P (Olsen P, <1.8) to identify low-P-tolerant and susceptible mutants.…”

Development and Genetic Characterization of A Novel Herbicide (Imazethapyr) Tolerant Mutant in Rice (Oryza sativa L.)

“…Linkage between the putatively linked marker and the target gene was estimated using genotypic data and the phenotypic data with the help of MAPMAKER v3.0 software (Lander et al 1987). Linkage was considered significant if the Logarithm of Odds (LOD) score was ≥3.0.…”