Efficacy of voriconazole and amphotericin B in corneal preservative media

Das, Sujata; Mitra, Sanchita; Garg, Prashant; Mallick, Aparajita; Priyadarshini, Smruti Rekha; Sharma, Savitri

doi:10.4103/ijo.ijo_1365_21

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…Kumar et al have developed nanostructured lipid carriers loaded with itraconazole and shown that this system can be an effective approach, with adequate activity against fungi and less local side effects [ 91 ]. While several in vitro and in vivo experimental studies have highlighted promising results using this treatment method, further controlled studies are necessary for its use on humans [ 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 ].…”

Section: Treatment Approachesmentioning

confidence: 99%

Recent Advances in Diagnosis and Treatment Approaches in Fungal Keratitis: A Narrative Review

Ghenciu,

Faur,

Bolintineanu

et al. 2024

Microorganisms

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Fungal keratitis represents a potentially sight-threatening infection associated with poor prognosis, as well as financial burden. Novel diagnostic methods include polymerase-chain-reaction (PCR)-based approaches, metagenomic deep sequences, in vivo confocal microscopy, and antifungal susceptibility testing. The ideal therapeutic approaches and outcomes have been widely discussed in recent times, with early therapy being of the utmost importance for the preservation of visual acuity, minimizing corneal damage and reducing the scar size. However, combination therapy can be more efficacious compared to monotherapy. Understanding the pathogenesis, early diagnosis, and prevention strategies can be of great importance. In this narrative, we discuss the recent progress that may aid our understanding of the diagnosis, treatment, and prevention of mycotic keratitis.

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Section: Treatment Approachesmentioning

confidence: 99%

Recent Advances in Diagnosis and Treatment Approaches in Fungal Keratitis: A Narrative Review

Ghenciu,

Faur,

Bolintineanu

et al. 2024

Microorganisms

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“…Efforts to prevent tissue contamination have led to improvement in disinfection protocols and reduced warming time during tissue processing [ 4 ]. Additionally, in vitro studies have demonstrated the effectiveness of antifungal supplementation in hypothermic storage [ 5 – 9 , 17 , 18 ], underscoring its potential in preventing fungal infections in EK procedures. Furthermore, recent studies suggest that antifungal supplementation could be cost-effective for EK surgeries [ 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

Investigating the Influence of Temperature and Supplementation Timing on Antifungal Efficacy in Storage Medium for Corneal Transplantation

Liu,

Chen,

Chu

et al. 2024

Ophthalmol Ther

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Introduction Although antifungal supplementation reduces the fungal load in the corneal storage medium, consensus is lacking on the influence of dosing and temperature. The study aims to evaluate the impact of eye bank warming protocol and timing of antifungal supplements on efficacy in Optisol-GS and tissue. Methods Corneoscleral rims contaminated with Candida albicans ( C. albicans ) were incubated in Optisol-GS, either without antifungal agents or with the addition of amphotericin B or voriconazole at various concentrations (2 ×, 5 ×, 10 ×, and 20 × MIC), at different time points, and under various preservation temperatures (2–8 °C versus 2 h-room temperature exposure). Antifungal efficacy was evaluated by counting viable yeast colonies cultured from Optisol-GS samples. Tissue sterility was determined through direct tissue culture and histological examination of the contaminated rims after a 14-day incubation period. Results Room temperature exposure did not increase colony growth at the same multiple MIC of antifungal agents. Although antifungal addition reduced C. albicans growth in a concentration-dependent manner, yeast growth was still observed in all Optisol-GS samples with a single supplementation after a 14-day incubation. Only groups with additional antifungal supplementation on either day 2 or day 6 showed a 99% or greater reduction of C. albicans growth in Optisol-GS samples and yielded negative results in direct tissue culture. Conclusions The eye bank warming protocol did not compromise antifungal efficacy. To sustain the required concentration and effectively reduce C. albicans growth in Optisol-GS and contaminated tissue, additional antifungal supplementation on either day 2 or day 6 was necessary during a 2-week preservation period. Supplementary Information The online version contains supplementary material available at 10.1007/s40123-024-00969-0.

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“…To provide a comprehensive overview of the current research, we have included a table [ Table 6 ] summarizing the key details from similar studies examining different antifungals. [ 16 21 22 ]…”

Section: Discussionmentioning

confidence: 99%

Anti-fungal efficacy of combination of amphotericin B with colistin and gentamicin in McCarey-Kaufman corneal preservation medium

Kukutam,

Chaurasia,

Joseph

et al. 2023

Indian Journal of Ophthalmology

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Purpose: To curtail the potential of donor corneal tissue disseminating fungi to the recipient’s eye, we evaluated the addition of amphotericin B to McCarey-Kaufman (M-K)—corneal storage medium supplemented with colistin. Methods: Amphotericin B was examined for its ability to inhibit the growth of Candida albicans and Aspergillus flavus using a microbroth dilution test and checkerboard assay in combination with only gentamicin and a combination of colistin, gentamicin, and amphotericin B. The safety on epithelium and endothelium was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The minimal inhibitory concentration of gentamicin was found to be >256 μg/ml against both C. albicans and A. flavus, whereas that of amphotericin B was found to be in a range of 0.25–0.5 and 1–2 μg/ml for C. albicans and A. flavus, respectively. According to the checkerboard assay, 80% (4/5) of C. albicans isolates and 100% (5/5) of A. flavus isolates responded synergistically to the combination of amphotericin B and gentamicin, but only 20% (1/5) of C. albicans isolates showed an additive effect. None of the tested isolates displayed antagonism. The combined effect of the three drugs also did not display any antagonistic effect. Additionally, the MTT assay reveals no toxic effect of the antimicrobials used on corneal epithelial and endothelial cells. Conclusion: In vitro experiments demonstrate that amphotericin B is not toxic to either epithelium or endothelium and is a promising additive to the M-K medium supplemented with colistin.

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Efficacy of voriconazole and amphotericin B in corneal preservative media

Cited by 6 publications

References 29 publications

Recent Advances in Diagnosis and Treatment Approaches in Fungal Keratitis: A Narrative Review

Recent Advances in Diagnosis and Treatment Approaches in Fungal Keratitis: A Narrative Review

Investigating the Influence of Temperature and Supplementation Timing on Antifungal Efficacy in Storage Medium for Corneal Transplantation

Anti-fungal efficacy of combination of amphotericin B with colistin and gentamicin in McCarey-Kaufman corneal preservation medium

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