Efficacy of two plant extracts against acne vulgaris: Initial results of microbiological tests and cell culture studies

Abstract

Summary Background Acne vulgaris is a common skin disease characterized by increased sebum production, inflammation, and colonization of Propionibacterium acnes (P. acnes) on pilosebaceous follicles. Aims To determine the efficacy of two different plant extracts against P. acnes and to analyze the gene expression levels of IL‐1α, SRD5A1, and TNFα in HaCaT cells treated with these plant extracts. Methods Anti‐acne extract 1 (AE1) consisted of Juglans regia (walnut husk), Myrtus communis (myrtle leaves), Matricaria chamomilla (chamomilla flowers), Urtica dioica (stinging nettle leaves), and Rosa damascena (rose flowers). Anti‐acne extract 2 (AE2) contained Brassica oleracea var. botrytis (broccoli) and B. oleracea var. italica (cauliflower). The antimicrobial activities of the extracts were tested on two different P. acnes strains: the reference strain of P. acnes (ATCC 51277) and the clinical isolate from a patient. The minimum inhibitory concentration (MIC) of the extracts was determined using the broth dilution method. Human keratinocyte cells were used for in vitro tests. Gene expression analyses were performed with RT‐qPCR. Results The MIC values of the extracts were below 1/2048 µg/mL. In the gene expression analysis, AE1 increased the expression level of TNFα (1.1719, P < 0.0001), suppressed the expression level of IL‐1α, SRD5A1 (0.0588, P = 0.0231; 0.3081, P = 0.0351), respectively. AE2 suppressed gene expression level of IL‐1α, SRD5A1, TNFα (0.3815, P = 0.0254; 0.3418, P = 0.0271; 0.1997, P = 0.0623). Conclusions Both herbal extracts demonstrated strong antibacterial and anti‐inflammatory activity in this preliminary trial. In conclusion, the topical application of these botanical extracts can be good candidates for local acne treatment.

<p>Clinical Assessment of New Topical Cream Containing Two Essential Oils Combined with Tretinoin in the Treatment of Acne</p>

“…18 The myrtle in previous studies has also shown in vitro a potential anti-acne activity associated with other plant extracts19 or antibiotics. 19,20 The anti-inflammatory action of the 2 essential oils has been demonstrated in vitro by other authors. 21,22 In our study, MOTC was compared to a commercial product (Acnatac) containing clindamycin and tretinoin whose anti-acne efficacy has already been demonstrated previously.…”

Polyherbal anti acne gel containing extracts of Mangifera indica and Syzygium cumini seeds: bioassay guided activity against Propionibacterium acne

“…A positive control, containing media with 0.1ml inoculum was maintained to indicate the growth promotion capacity of the media. Test samples of Propionibacterium acnes were incubated under anaerobic condition in an anaerobic jar (Hi-Media) with gas pack for 48h [8,[9][10][11][12][13][14][15].…”

“…Then Triethanolamine was added dropwise until the gel consistency and pH is adjusted between 6.8 to 7.4. The final volume was made up to 200g by the addition of distilled water [9][10][11][12][13][14][15][16][17][18][19]. Physical evaluation of gel.…”

Use of elicitation in the cultivation of Bimi® for food and ingredients

“…18 Furthermore, not only the ingredient by itself, but also Brassica-derived extracts are being used in the food industry as nutraceuticals, for food storage, or as prototype ingredients in the cosmetic industry. [19][20][21] However, these extracts are usually based in ITCs content with a lower shelf-life stability than GLSs. 22,23 The phytochemical composition of plant foods can be enriched by using elicitors, such as jasmonic acid and its methyl ester (methyl jasmonate, MeJA).…”

“…More recently, additional roles of the bioactives in Brassica species, in relation to diabetes and obesity, have been reported in preclinical studies and in nutritional interventions . Furthermore, not only the ingredient by itself, but also Brassica ‐derived extracts are being used in the food industry as nutraceuticals, for food storage, or as prototype ingredients in the cosmetic industry . However, these extracts are usually based in ITCs content with a lower shelf‐life stability than GLSs …”